骨髓源神经祖细胞诱导小胶质细胞自噬及其潜在分子机制探究

doi:10.13418/j.issn.1001-165x.2021.03.011

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (3): 302-310.doi: 10.13418/j.issn.1001-165x.2021.03.011

骨髓源神经祖细胞诱导小胶质细胞自噬及其潜在分子机制探究

员凤英¹，　张明兴¹，　石艺华¹，　李媚慧²，　区家源²，　张鸣生²，　白文芳²

1.广东药科大学附属第一医院康复医学科，广州 510062；　2.广东省人民医院康复医学科，广州 510080

收稿日期:2020-09-14 出版日期:2021-05-25 发布日期:2021-06-02
通讯作者: 白文芳，主治医师，博士，E-mail：freedombwf1008@126.com；张鸣生，教授，博士研究生导师，E-mail：mszrch@163.com
作者简介:员风英（1979-），女，主治医师，硕士，研究方向：神经康复医学，E-mail：bstg666@yeah.net
基金资助:
国家自然科学基金（81871842）；广东省医学科研基金（A2018510）；广东省中医药局面上科研项目（20192045）

Research on the microglial cell autophagy induced by bone marrow stromal cells and its potential molecular mechanism

Yuan Fengying¹, Zhang Mingxing¹, Shi Yihua¹, Li Meihui², Ou Jiayuan², Zhang Mingsheng², Bai Wenfang ²

1. Department of Rehabilitation Medicine, The First Affiliated Hospital, Guangdong Pharmaceutical University, Guangzhou 510062, China; 2. Department of Rehabilitation Medicine, Guangdong Provincial People's Hospital, Guangzhou 510080, China

Received:2020-09-14 Online:2021-05-25 Published:2021-06-02

摘要/Abstract

摘要： 目的　探讨骨髓源神经祖细胞诱导小胶质细胞自噬的可能，通过对其代谢物的鉴定及外泌体的分离明确潜在影响小胶质细胞生长的分子机制。方法　采用细胞生物学技术，电镜检测，结合LCMS/MS检测技术，鉴定骨髓源神经祖细胞中的脂质体代谢物类型。结果　外泌体可以诱导小胶质细胞发生自噬。骨髓源神经祖细胞培养基中检测到4597种质核比的潜在物质，检测到304种已知的脂质体代谢物质，统计计算得到51种潜在骨髓源神经祖细胞生长过程中潜在代谢分泌的脂质体物质。这些物质都是潜在可引起小胶质细胞发生自噬的物质。结论　骨髓源神经祖细胞可以诱导小胶质细胞发生自噬现象，促进免疫反应的启动，这一过程中骨髓源神经祖细胞外泌体中携带的脂质体代谢物可能发挥了重要作用。

关键词: 骨髓源神经祖细胞；　外泌体； , , 小胶质细胞； , , 自噬； , , 脂质体代谢

Abstract: Objective　To explore the possibility of bone marrow derived neural progenitor cells inducing microglia autophagy, and to identify the molecular mechanisms that might affect microglia growth through the identification of its metabolites and the isolation of exosomes.　Methods　The effects of exosomes on microglia were observed by cell biology and electron microscopy. The liposome metabolite types were identified by chemical reagent extraction and LCMS/MS detection technology.　Results　Molecular experiments have shown that this exosome can induce autophagy in microglia. The potential substances with the ratio of 4597 kinds of germplasm nuclei were detected in the culture medium of bone marrow-derived nerve progenitor cells, 304 kinds of known liposome metabolites were detected in the culture medium and bone marine-derived nerve progenitor cells. After excluding the inherent liposome metabolites in the culture medium, 51 kinds of potentially metabolized and secreted liposomes in the growth process of bone marrow derived nerve progenitor cells were obtained. These were all substances that can potentially cause autophagy in microglia.　Conclusions　Bone marrow derived nerve progenitor cells can induce autophagy in microglia and promote the initiation of immune response, during which liposome metabolites carried in exosomes of bone marrow derived nerve progenitor cells may play an important role.

Key words: Bone marrow stromal cells; , Exosomes; , Microglial cell;　Autophagy;　Lipid metabolites

中图分类号:

员凤英, 张明兴, 石艺华, 李媚慧, 区家源, 张鸣生, 白文芳. 骨髓源神经祖细胞诱导小胶质细胞自噬及其潜在分子机制探究[J]. 中国临床解剖学杂志, 2021, 39(3): 302-310.

Yuan Fengying, Zhang Mingxing, Shi Yihua, Li Meihui, Ou Jiayuan, Zhang Mingsheng , Bai Wenfang. Research on the microglial cell autophagy induced by bone marrow stromal cells and its potential molecular mechanism [J]. Chinese Journal of Clinical Anatomy, 2021, 39(3): 302-310.

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骨髓源神经祖细胞诱导小胶质细胞自噬及其潜在分子机制探究

Research on the microglial cell autophagy induced by bone marrow stromal cells and its potential molecular mechanism

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