中老年小鼠通过长期运动改善Aβ1-42诱导的认知功能障碍

doi:10.13418/j.issn.1001-165x.2022.1.08

中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (1): 39-44.doi: 10.13418/j.issn.1001-165x.2022.1.08

中老年小鼠通过长期运动改善Aβ1-42诱导的认知功能障碍

张卫国，　杨娜，　杨静，　刘雪芹，　赵云鹤*

山西医科大学人体解剖学教研室，太原 030001

收稿日期:2021-10-03 出版日期:2022-01-25 发布日期:2022-01-19
通讯作者: 赵云鹤，博士，副教授，E-mail：yunhezhao@sxmu.edu.cn
作者简介:张卫国（1978-），男，山西太原人，讲师，硕士，研究方向：神经系统疾病预防与控制，E-mail：13934588509@139.com
基金资助:
国家自然科学基金项目（81571381）；山西省回国留学人员科研资助项目（2020-085）；山西省青年科技研究基金（201801D221407）；山西省高等学校教学改革创新项目（J2020094）

Long-term exercise ameliorates cognitive disorder induced by Aβ1-42 in middle-aged and elderly mice

Zhang Weiguo, Yang Na, Yang Jing, Liu Xueqin, Zhao Yunhe*

Department of Human Anatomy, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China

Received:2021-10-03 Online:2022-01-25 Published:2022-01-19

摘要/Abstract

摘要： 目的　观察中老年小鼠通过长期自主跑轮运动改善β淀粉样蛋白1-42（Aβ1-42）所致认知功能障碍及其机制。方法　中老年BABL/c小鼠（12月龄）随机分为4组，①溶剂对照静坐组（VS），②溶剂对照长期自主跑轮运动组（VR），③Aβ1-42静坐对照组（AS），④Aβ1-42长期自主跑轮运动组（AR），按分组条件分别予以自主跑轮或静坐6个月，双侧海马注射Aβ1-42或等量溶剂。注射两周后进行相关检测。结果　Western blot显示中老年小鼠长期运动致海马泛素化蛋白下降0.2倍（P<0.05）。新物体识别实验显示AR组新物体识别指数高于AS组0.42倍（P<0.05）；Y迷宫自发交替实验显示AR组的入臂正确率较AS组升高0.21倍（P<0.05）。荧光分光光度检测显示AR组海马蛋白酶体活性高于AS组0.18倍（P<0.05）；Western blot显示AR组小鼠海马泛素化蛋白沉积较AS组小鼠减少0.21倍（P<0.05）；免疫组化结果显示AR组Aβ1-42沉积减少（P<0.01）。结论　中老年小鼠长期自主跑轮运动可维持海马蛋白酶体活性，改善Aβ1-42导致的认知功能障碍。

关键词: 中老年小鼠, 　长期运动, 　β淀粉样蛋白1-42, 　蛋白酶体活性, 　认知障碍

Abstract: Objective　To observe the mechanism of cognitive dysfunction caused by Aβ-amyloid 1-42 (Aβ1-42) in middle-aged and elderly mice by long-term exercise.　Methods 　12-month-old BABL/c mice were randomly divided into the following four groups:①a vehicle control sedentary group (VS),②a vehicle control long-term voluntary running wheel exercise group (VR), ③an Aβ1-42 sedentary group (AS),④an Aβ1-42 long-term voluntary running wheel exercise group (AR). Voluntary running wheel exercise or sedentary for 6 months was performed according to the grouping conditions. The bilateral hippocampus was injected with Aβ1-42 or equivalent solvent for 6 months. Tests were performed two weeks after injection. Results　Western blotting results showed that the accumulation of ubiquitinated protein in the hippocampus of 12-month-old BABL/c mice after long-term voluntary exercise was significantly lower than that in the sedentary group by about 0.2 times (P<0.05). The novel object recognition experiment showed that the preference index in the AR group was 0.42 times higher than that in the AS group（P<0.05). The spontaneous alternation experiment of Y maze showed that the correct rate of arm entry in AR group increased than that in AS group by about 0.21 times (P<0.05). The proteasome activity of AR hippocampus was significantly higher than that of AS group by about 0.18 times (P<0.05). Western blotting results showed the accumulation of ubiquitinated protein in the hippocampus in the AR group was significantly lower than that of AS group by about 0.21 times (P<0.05). Immunohistochemical results showed that Aβ1-42 deposition in AR group was lesser（P<0.01).　Conclusions Middle-aged and elderly mice can maintain hippocampal proteasome activity and improve the cognitive dysfunction caused by Aβ1-42 through long-term voluntary running wheel exercise.

Key words: Middle-aged and elderly mice, 　Long-term exercise, 　Aβ1-42, 　Proteasome activity, Cognitive dysfunction

中图分类号:

R322.8

张卫国, 　杨娜, 　杨静, 　刘雪芹, 　赵云鹤. 中老年小鼠通过长期运动改善Aβ1-42诱导的认知功能障碍[J]. 中国临床解剖学杂志, 2022, 40(1): 39-44.

Zhang Weiguo, Yang Na, Yang Jing, Liu Xueqin, Zhao Yunhe. Long-term exercise ameliorates cognitive disorder induced by Aβ1-42 in middle-aged and elderly mice[J]. Chinese Journal of Clinical Anatomy, 2022, 40(1): 39-44.

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中老年小鼠通过长期运动改善Aβ1-42诱导的认知功能障碍

Long-term exercise ameliorates cognitive disorder induced by Aβ1-42 in middle-aged and elderly mice

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