中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (3): 271-276.doi: 10.13418/j.issn.1001-165x.2022.3.06

• 应用解剖 • 上一篇    下一篇

大鼠腹壁浅动脉皮瓣游离移植至腋窝模型的构建技巧

吴东方1,    刘东红2,    方芳3,    荆幸3,    陈绍锋3,    庄跃宏3*   

  1. 1. 温州医科大学检验医学院(生命科学学院),  浙江   温州   325035;    2.山东第一医科大学第二附属医院, 山东  泰安   271000
    3.福建医科大学基础医学院人体解剖学系,  福州   350122
  • 收稿日期:2021-08-11 出版日期:2022-05-25 发布日期:2022-05-31
  • 通讯作者: 庄跃宏,博士,副教授,E-mail:zhuangyuehong@163.com
  • 作者简介:吴东方(1987-),女,浙江温州人,硕士,主要从事解剖学方面的研究,E-mail:2602350526@qq.com
  • 基金资助:
    浙江省医药卫生科技计划项目(2019KY447)

Establishment of the groin flap based on the superficial epigastric artery into axillary fossa in rats

Wu Dongfang1, Liu Donghong2, Fang Fang3, Jing Xing3, Chen Shaofeng3, Zhuang Yuehong3*   

  1. 1. College of laboratory Medicine (College of Life Sciences), Wenzhou Medical University, Wenzhou 325035, Zhejiang Province, China;  2. Second Affiliated Hospital, Shandong First Medical University, Tai'an 271000, Shandong Province,China;  3. Department of Human Anatomy, School of Basic Medicine, Fujian Medical University, Fuzhou 350122, China 
  • Received:2021-08-11 Online:2022-05-25 Published:2022-05-31

摘要: 目的    推介将大鼠腹壁浅动脉皮瓣游离移植至腋窝的具体操作步骤。  方法    选取体重420 ~ 450 g的清洁级SD雄性大鼠20只,麻醉成功后备皮,设计以腹壁浅血管为蒂的4 cm × 2 cm腹股沟皮瓣,游离移植至腋窝,以胸外侧血管为受区血管,重点观察:股血管及胸外侧血管的起源、走行与分支,测量其外径和长度;大鼠生存状态;术后第7 d皮瓣存活情况。  结果    股动、静脉的管径分别为(0.72± 0.08)mm及(1.29±0.17)mm,胸外侧动、静脉的管径分别为(0.59±0.18)mm及(1.04±0.12)mm,两者适合做端端吻合。术后有2只大鼠死亡,另有3只大鼠皮瓣完全坏死,皮瓣存活率达75%。由于股动脉的破坏,患肢术后会出现跛行,1周后,随着血供的代偿,大鼠的步态逐渐恢复正常。  结论   大鼠腹壁浅动脉游离皮瓣是超级显微外科的基础研究与临床前操作练习的有效模型之一。

关键词: 游离皮瓣,  ,  , 腹壁浅动脉,  ,  , 胸外侧动脉,  ,  , 血管吻合

Abstract: Objective To describe the procedure of transfer of the groin flap based on the superficial epigastric artery into the axillary fossa in rats.   Materials   Twenty clean SD male rats weighing 420~450 g were depilated after successful anesthesia. A free groin flap model with a size of 4cm×2cm was established based on the superficial blood vessel of the abdominal wall was transplanted to the axillary fossa with the lateral thoracic vessels used as the recipient vessels. The main observations were as follows: ① the origin, course and branches of femoral vessels and lateral thoracic vessels were observed, and their outer diameter and length were measured; ② survival state of experimental animals; ③ The survival of the flap on the 7th day after operation.    Results    The diameter of the femoral artery and vein was (0.72±0.08) mm and (1.29±0.17) mm respectively, and that of the lateral thoracic artery and vein was (0.59±0.18) mm and (1.04±0.12) mm respectively. Two rats failed to recover from anesthesia after operation, and 3 rats had complete necrosis of skin flap, resulting in a flap survival rate of 75%. Due to removal of the femoral artery, claudication of the affected limb could occur after surgery.  One week later, with compensation of the blood supply, the gait of rats gradually returned to normal.    Conclusions   The free flap based on the superficial epigastric artery in rats is very suitable for junior doctors and researchers who want to engage in the clinical work and the basic research relating to supramicrosurgery.

Key words: Free flap,  ,  , Superficial epigastric artery,  ,  , Common thoracic artery,  ,  , Vascular anastomosis 

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