冬凌草甲素通过上调miR-204-5p抑制PC-3细胞迁移与侵袭的作用机制研究

doi:10.13418/j.issn.1001-165x.2022.3.08

中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (3): 286-291.doi: 10.13418/j.issn.1001-165x.2022.3.08

冬凌草甲素通过上调miR-204-5p抑制PC-3细胞迁移与侵袭的作用机制研究

施浩然¹#，包广龙^1#，令狐熙涛¹，吴洪瀚¹，赵泽驹²，刘霄³，付瑶⁴，黄帅⁵*，瓦庆德¹*

1.遵义医科大学第二附属医院骨科，贵州遵义 563000； 2.遵义医科大学附属医院泌尿外科，贵州遵义 563003；
3.遵义医科大学第二附属医院肿瘤科，贵州遵义 563003； 4.四川大学华西第二医院康复医学科，成都 610041；
5.广州医科大学第二附属医院骨科，广州 510260

收稿日期:2021-06-26 出版日期:2022-05-25 发布日期:2022-05-31
通讯作者: 黄帅，医学博士，副教授，E-mail：huangshuai85315@163.com；瓦庆德，教授，主任医师，博士研究生导师，E-mail：wqd887zsy@126.com
作者简介:施浩然（1995-），男，四川人，硕士研究生，研究方向：前列腺癌骨转移；E-mail：shr123456zxc@163.com；共同第一作者:包广龙（1996-），男，贵州人，硕士研究生，研究方向：前列腺癌骨转移 E-mail：baogl1996@163.com
基金资助:
国家自然科学基金（81860322）；贵州省科技计划项目（黔科合基础-ZK[2021]重点007）；广东省基础与应用基础研究基金区域联合基金-重点项目（2020B1515120094）

Study on the mechanism of oridonin inhibiting PC-3 cells migration and invasion by up-regulating miR-204-5p

Shi Haoran^1#, Bao Guanglong^1#, LingHu Xitao¹, Wu Honghan¹, Zhao Zeju², Liu Xiao³, Fu Yao⁴ , Huang Shuai⁵*, Wa Qingde¹*

1.Department of Orthopedics, the Second Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China; 2. Department of Urology, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China; 3. Department of Oncology, the Second Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China; 4. Department of Rehabilitation Medicine, West China Second University Hospital, Sichuan University, Chengdu 610041, China; 5. Department of Orthopedics, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China

Received:2021-06-26 Online:2022-05-25 Published:2022-05-31

摘要/Abstract

摘要： 目的探索冬凌草甲素影响PC-3细胞迁移及侵袭的作用及机制，为冬凌草甲素治疗前列腺癌骨转移提供前期体外实验参考。方法通过CCK8检测冬凌草甲素对PC-3细胞增殖的影响，Transwell检测冬凌草甲素对PC-3细胞迁移和侵袭的影响，qRT-PCR分析冬凌草甲素溶液处理PC-3细胞后miR-204-5p及NF-κB信号通路表达情况，Western blotting分析冬凌草甲素溶液处理PC-3细胞后NF-κB信号通路蛋白表达情况。结果冬凌草甲素溶液以浓度依赖性和时间依赖性方式抑制PC-3细胞的增殖，与对照组相比差异有统计学意义（P<0.01），冬凌草甲素IC50值为10 μmol/L；冬凌草甲素溶液呈浓度依赖性抑制PC-3细胞的迁移和侵袭，差异较对照组有统计学意义（P<0.01）；冬凌草甲素溶液能促进PC-3细胞表达miR-204-5p并阻断NF-κB信号通路下游基因表达；过表达miR-204-5p和冬凌草甲素均能抑制PC-3细胞NF-κB信号通路下游基因表达。结论冬凌草甲素能抑制PC-3细胞的增殖、迁移及侵袭，其作用机制可能与其能上调miR-204-5p阻断NF-κB信号通路有关。

关键词: 冬凌草甲素； , , 前列腺癌骨转移； , , miR-204-5p； , , NF-κB信号通路

Abstract: 【Abstract】 Objective To explore the effect and mechanism of oridonin on the migration and invasion of PC-3 cells, to provide preliminary in vitro experiment reference for the treatment of bone metastasis of prostate cancer with oridonin. Methods The effect of oridonin on the proliferation of PC-3 cells was detected by CCK8. The effect of oridonin on the migration and invasion of PC-3 cells was detected by the Transwell test. The expression of miR-204-5p and NF-κB signaling pathways after the treatment of PC-3 cells with oridonin was analyzed by qRT-PCR. The expression of protein in the NF-κB signaling pathway after the treatment of PC-3 cells with oridonin was analyzed by Western blotting. Results Firstly, the proliferation of PC-3 cells was inhibited by oridonin solution in a concentration- and time-dependent manner; and the difference from the control group was of statistical significance (P<0.01), the IC50 value of oridonin was 10 μmol/L. Secondly, the migration and invasion of PC-3 cells were inhibited by oridonin in a concentration-dependent manner; and the difference from the control group was of statistical significance (P<0.01). Finally, the results of qRT-PCR showed that oridonin solution promoted the expression of miR-204-5p in PC-3 cells and blocked the expression of the downstream genes in the NF-κB signaling pathway; the results of Western blotting indicated that both the overexpression of miR-204-5p and oridonin could inhibit the expression of the downstream genes in NF-κB signaling pathway in PC-3 cells. Conclusions Oridonin can inhibit the proliferation, migration and invasion of PC-3 cells. Its mechanism of action may be associated with its up-regulating miR-204-5p and blocking the NF-κB signaling pathway.

Key words: Oridonin; , , Prostate cancer with bone metastasis; , , miR-204-5p; , , NF-κB signaling pathway

中图分类号:

R34
R73-37

施浩然, 包广龙, 令狐熙涛, 吴洪瀚, 赵泽驹, 刘霄, 付瑶, 黄帅, 瓦庆德. 冬凌草甲素通过上调miR-204-5p抑制PC-3细胞迁移与侵袭的作用机制研究[J]. 中国临床解剖学杂志, 2022, 40(3): 286-291.

Shi Haoran, Bao Guanglong, LingHu Xitao, Wu Honghan, Zhao Zeju, Liu Xiao, Fu Yao , Huang Shuai, Wa Qingde. Study on the mechanism of oridonin inhibiting PC-3 cells migration and invasion by up-regulating miR-204-5p [J]. Chinese Journal of Clinical Anatomy, 2022, 40(3): 286-291.

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冬凌草甲素通过上调miR-204-5p抑制PC-3细胞迁移与侵袭的作用机制研究

Study on the mechanism of oridonin inhibiting PC-3 cells migration and invasion by up-regulating miR-204-5p

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