链脲佐菌素对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响

doi:10.13418/j.issn.1001-165x.2023.2.11

中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (2): 182-186.doi: 10.13418/j.issn.1001-165x.2023.2.11

链脲佐菌素对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响

孟星圻¹，邓靖²，彭莉轩¹，张圆³，林祎³，周小兵¹，曹文宇¹*，李素云¹*

南华大学衡阳医学院 1.应用解剖学与生殖医学研究所， 2.临床医学，3.肿瘤研究所，
肿瘤细胞和分子病理学湖南省重点实验室，湖南衡阳 421001

收稿日期:2021-09-13 出版日期:2023-03-25 发布日期:2023-04-11
通讯作者: 李素云，E-mail：lsy1631632021@163.com；曹文宇，E-mail：marksman0@163.com
作者简介:孟星圻（1998-），女，贵州贵阳人，在读硕士，主要从事生殖方面的研究，E-mail：1020899103@qq.com
基金资助:
湖南省自然科学基金（2021JJ30593）；湖南省教育厅重点基金（19A428）；湖南省大学生研究性学习与创新性实验项目（S20201 0555091）；南华大学大学生研究性学习与创新性实验项目（X20201055 5380）

Effects of streptozotocin on spermatogenic function and testicular m6A methylase expression in diabetic mice

Meng Xingqi ¹, Deng Jing², Peng Lixuan¹, Zhang Yuan ³, Lin Yi ³, Zhou Xiaobing ¹, Cao Wenyu ¹*, Li Suyun¹*

Hengyang Medical School, University of South China 1.Clinical Anatomy & Reproductive Medicine Application Institute; 2. Clinical Medicine; 3.Hunan Provincial Key Laboratory of Tumor Cell and Molecular Pathology, Cancer Institute; Hengyang 421001 , Hunan Province, China

Received:2021-09-13 Online:2023-03-25 Published:2023-04-11

摘要/Abstract

摘要： 目的探讨链脲佐菌素（streptozotocin，STZ）对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响。方法 20只8周龄雄性ICR小鼠，随机分为溶媒对照组和模型组，腹腔注射STZ诱导糖尿病动物模型。注射后第2、4、6、8周检测血糖及体重；第8周采用精子计数法检测小鼠精子数量，HE染色检测精子存活率及睾丸形态，称量睾丸、附睾的重量；Real-time PCR和Western blotting检测各组小鼠睾丸中m6A甲基化酶mRNA及蛋白表达。结果与对照组相比，模型组小鼠空腹血糖显著增高，体重降低（P<0.01）；睾丸及附睾重量显著下降（P<0.01）、精子数量显著下降（P<0.05）；睾丸形态结构破坏，生精小管萎缩，管腔直径变小，各级生精细胞排列紊乱，管腔内精子数量减少；睾丸m6A甲基化酶METTL3、FTO、YTHDF3 mRNA表达降低（P<0.05）；METTL3、FTO蛋白质表达显著降低（P<0.05）。结论 STZ诱导的糖尿病小鼠出现生精障碍，其生精障碍可能与睾丸内m6A甲基化酶METTL3以及FTO的表达异常有关。

关键词: m6A甲基化； , , 链脲佐菌素； , , 糖尿病； , , 生精功能障碍

Abstract: Objective To investigate the effects of streptozotocin (STZ) on spermatogenic function and testicular m6A methylase expression in diabetic mice. Methods Twenty 8-week-old male ICR mice were randomly divided into a vehicle control group (NS) and a model group (STZ), and an animal model of diabetes was induced by intraperitoneal injection of STZ. Blood glucose and body weight changes were measured on the 2nd, 4th, 6th, 8th week after STZ injection. On the 8th week, testes and epididymis were weighed and the number of sperm was also counted, HE staining was then used to detect sperm motility and testicular morphology changes. Real-time PCR and Western blotting methods were used to detect the expression level of m6A methylase mRNA and protein in the testis of each group of mice. Results Compared with the NS group, the fasting blood glucose was significantly increased and body weight was significantly decreased in the model group (P<0.01), the weight of the testis and epididymis was significantly decreased (P<0.01), and the number of sperm was significantly decreased (P<0.05); the morphological structure of the testis was destroyed, the seminiferous tubules shrinked, the diameter of the lumen became smaller, the arrangement of spermatogenic cells at all levels was disordered, and the number of sperm in the lumen decreased; the expression of testicular m6A methylases METTL3, FTO, and YTHDF3 mRNA were significantly decreased (P<0.05); the expression of METTL3 and FTO protein were significantly reduced (P<0.05). Conclusions The spermatogenesis disorder in STZ-induced diabetic mice may be related to the abnormal expression of m6A methylase METTL3 and FTO in the testis.

Key words: m6A methylation; , , Streptozotocin; , , Diabetes; , , Spermatogenic dysfunction

中图分类号:

R322.64

孟星圻, 邓靖, 彭莉轩, 张圆, 林祎, 周小兵, 曹文宇, 李素云. 链脲佐菌素对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响[J]. 中国临床解剖学杂志, 2023, 41(2): 182-186.

Meng Xingqi, Deng Jing, Peng Lixuan, Zhang Yuan, Lin Yi, Zhou Xiaobing, Cao Wenyu, Li Suyun. Effects of streptozotocin on spermatogenic function and testicular m6A methylase expression in diabetic mice[J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 182-186.

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链脲佐菌素对糖尿病小鼠生精功能和睾丸m6A甲基化酶表达的影响

Effects of streptozotocin on spermatogenic function and testicular m6A methylase expression in diabetic mice

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