少阳生骨方对SD大鼠胫骨骨折断端18F-NaF分布的影响

doi:10.13418/j.issn.1001-165x.2018.05.013

中国临床解剖学杂志 ›› 2018, Vol. 36 ›› Issue (5): 545-550.doi: 10.13418/j.issn.1001-165x.2018.05.013

少阳生骨方对SD大鼠胫骨骨折断端18F-NaF分布的影响

郭晓光^{1, 2}，　关钛元^{1, 2}，　张磊^{1, 2}，　扶世杰^{1, 2}，　周鑫^{1, 2}，　刘刚^{1, 2}，　陈跃³

1. 西南医科大学附属中医医院，四川泸州 646000；　2. 泸州市院士工作站，四川泸州 646000；
3.西南医科大学附属医院核医学科，四川泸州 646000

收稿日期:2018-05-11 出版日期:2018-09-25 发布日期:2018-10-26
通讯作者: 张磊，博士，主治医师，E-mail: zhanglei870722@126.com
作者简介:并列第一作者：郭晓光（1992-），在读硕士，E-mail:1204132080@qq.com；关钛元（1983-），医学硕士，主治医师，擅长骨关节疾病的中西医结合治疗，E-mail:13882766387@163.com
基金资助:
四川省科技支撑计划项目（2014SZ0185）；泸州市院士工作站在建项目（20180101）；泸州市科技计划项目（2014-S-46 (8/11)）；西南医科大学-西南医科大学附属中医医院联合专项项目（2016-4-31）

Effects of ShaoYangShengGuFang Prescription on the distribution of 18F-NaF in rat tibial fracture bone

GUO Xiao-guang ^1,2, GUAN Tai-yuan ^1,2, ZHANG Lei^1,2, FU Shi-jie ^1,2, ZHOU Xin ^1,2, LIU Gang ^1,2, CHEN Yue³

1.The Affiliated T.C.M Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province,China; 2.Academician Workstation in Luzhou, Sichuan Province, China; 3.Department of Nuclear Medicine, The Affiliated Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China

Received:2018-05-11 Online:2018-09-25 Published:2018-10-26

摘要/Abstract

摘要：

目的　探讨18F-NaF在评价骨折早期愈合方面的应用价值，以及少阳生骨方对大鼠骨折愈合的影响。方法　将72只SD大鼠进行左侧胫骨骨折造模，造模后随机分为实验组（少阳生骨方汤剂灌胃）、对照组（骨肽片悬浮液灌胃）和空白组（0.9%生理盐水灌胃），每组24只。在7、15、30、56 d从3组中各随机选取6只大鼠，对胫骨骨折断端进行PET/CT扫描，计算骨折断端对18F-NaF的摄取。结果　实验组和对照组18F-NaF摄取值于15 d达到峰值（9.00±0.42）%ID/g、（8.10±0.71）%ID/g，而空白组18F-NaF摄取值于30 d达到峰值（4.25±0.35）%ID/g，且在四个不同时间点，实验组摄取量与对照组间差异无统计学意义（P>0.05）；实验组和对照组较空白组摄取量高，差异有统计学意义（P<0.05）。结论　18F-NaF在评估早期骨折愈合中具有一定的应用价值，少阳生骨方能够促进大鼠胫骨骨折愈合。

关键词: 少阳生骨方, 　18F-NaF, 　正电子发射断层显像, 　骨折愈合, 　大鼠

Abstract:

Objective　To explore the application value of 18F-NaF in evaluation of early fracture healing, and the effects of ShaoYangShengGuFang Prescription on rat fracture healing.　Methods　72 SD rats were performed left tibial fracture modeling. The model rats were randomly divided into experimental group (intragastric administration of ShaoYangShengGuFang Prescription)，control group (intragastric administration of Ossotide Tablets) and blank group (intragastric administration of 0.9% saline). Every group included 24 rats. After 7, 15, 30, and 56 days, 6 rats were randomly selected from each of the three groups. The PET/CT scan was performed on the fractured ends of tibial fractures, and the uptake of 18F-NaF at the fracture end was calculated.　Results　The 18F-NaF uptake of experimental group and control group reached their peaks at the 15th day (9.00±0.42 %ID/g、8.10±0.71 %ID/g), while the 18F-NaF uptake of blank group reached its peak at the 30th day (4.25±0.35 %ID/g). At four different time points, there was no significant difference between the uptake of the experimental group and that of the control group in the 18F-NaF uptake of rat tibial fracture bone (P>0.05), while the 18F-NaF uptake of the experimental group or the control group was higher than that of the blank group, which was of remarkable statistical difference (P<0.05). Conclution　18F-NaF has some values in evaluating early fracture healing, and ShaoYangShengGuFang Prescription can promote the healing of tibial fractures in rats.

Key words: ShaoYangShengGuFang Prescription, 　18F-sodium fluoride, 　PET/CT, 　Fracture healing, 　Rat

郭晓光,关钛元,张磊,扶世杰,周鑫,刘刚,陈跃. 少阳生骨方对SD大鼠胫骨骨折断端18F-NaF分布的影响[J]. 中国临床解剖学杂志, 2018, 36(5): 545-550.

GUO Xiao-guang, GUAN Tai-yuan, ZHANG Lei, FU Shi-jie, ZHOU Xin, LIU Gang, CHEN Yue. Effects of ShaoYangShengGuFang Prescription on the distribution of 18F-NaF in rat tibial fracture bone[J]. Chinese Journal Of Clinical Anatomy, 2018, 36(5): 545-550.

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少阳生骨方对SD大鼠胫骨骨折断端18F-NaF分布的影响

Effects of ShaoYangShengGuFang Prescription on the distribution of 18F-NaF in rat tibial fracture bone

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