关键词: 磁压榨技术, 　气管食管瘘, 　动物模型, 　微创

Abstract:

Objective　To explore the feasibility of establishing ultra-invasive magnetic compression technology to establish a rabbit model of tracheal esophageal fistula(TEF).　Methods　Ten New Zealand rabbits were anesthetized and inserted into the parent magnet and daughter magnet in the cervical trachea and esophagus, respectively. X-ray was taken the following day to observe the location of the mother and daughter magnets. When the parent and daughter magnets were found to be out of the original position and entered the digestive tract, the animals were sacrificed three days later. The general specimens of tracheal esophagus were taken, and the tissue of the fistula was taken for HE and Masson staining for pathological observation.　Results　The average operation time of magnetic compression ultra-invasive technique to establish a TEF was (3.20 ± 0.81) min.  (6.90 ± 1.14) d after operation the magnets fell into the digestive tract, while TEF formatted. There was a slight adhesion between the tracheal membrane and the anterior wall of the esophagus, and the tissue around the fistula was observed. Histopathological observation showed that the stenosis was connected to the anterior wall of the esophagus, which was closely related to the pathological features of the clinically acquired tracheoesophageal fistula.　Conclusions　Establishment of rabbit TEF using magnetic compression ultra-minimally invasive technology is a simple, minimally invasive, and feasible method for animal model of TEF. It can be used as an ideal animal modeling method for obtaining TEF.

Key words: Magnetic compression technique, 　Tracheoesophageal fistula, 　Animal model, 　Minimal invasive