Genistein对子痫前期患者离体脐静脉内皮细胞的保护作用及其机制研究#br#

doi:10.13418/j.issn.1001-165x.2019.03.005

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (3): 261-266.doi: 10.13418/j.issn.1001-165x.2019.03.005

Genistein对子痫前期患者离体脐静脉内皮细胞的保护作用及其机制研究#br#

刘黎星¹，　闵羡慧²，　张敏¹，　侯赛榕¹

1.莆田学院基础医学部人体解剖与组胚学教研室，福建莆田 351100；
2.解放军福州总医院九五临床部，福建莆田 351100

收稿日期:2019-03-23 出版日期:2019-05-25 发布日期:2019-06-13
作者简介:刘黎星（1974-），女，福建莆田人，教授，医学博士，E-mail：llx5836@163.com
基金资助:
国自然青年基金（81502360）;莆田学院育苗基金（2016111,2014058）；福建省科技厅高校专项基金（2012D118）

Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms

LIU Li-xing¹ , MIN Xian-hui ², ZHANG Min¹, HOU Sai-rong¹

1. Department of Anatomy, Histology and Embryology, Basic Medical College of Putian University, Putian 351100, Fujian Province, China; 2. Department of Obstetrics and Gynecology, First Hospital Affiliated to Fuzhou General Hospital of Nanjing Military Command of PLA, Putian Fujian 351100, China

Received:2019-03-23 Online:2019-05-25 Published:2019-06-13

摘要/Abstract

摘要： 目的　分析染料木素（Genistein）对子痫前期患者脐静脉内皮细胞（HUVECs）数量及功能变化的影响及其机制。方法　选取同期住院、年龄孕周及病变程度无显著差异的子痫前期患者40例，随机分成Genistein干预组及患者组（每组20例），20例正常妊娠孕妇为对照组，分离并培养上述研究对象的HUVECs，采用MTT等方法检测3组内皮细胞的增殖、黏附和迁移能力，采用荧光标记的牛血清白蛋白（BSA）检测3组内皮细胞的单层屏障功能；同时采用 ELISA 法检测内皮细胞8-羟基脱氧鸟苷（8-OHdG）水平，比色法检测谷胱甘肽过氧化物酶（GSH-Px）水平，Real-time PCR和Western blot法检测凋亡相关的Bcl-2、Bax基因和蛋白表达的水平。结果　与子痫前期患者组比较，Genistein干预组内皮细胞增殖、黏附、迁移能力及其屏障功能明显升高，内皮细胞GSH-Px活性、抗凋亡的Bcl-2基因和蛋白表达水平明显升高，同时，8-OHdG水平及促凋亡的Bax基因和蛋白表达水平明显下降，且差异均有显著性（P<0.01)。而Genistein干预组与正常妊娠组比较，上述差异无显著性。结论　Genistein可能通过抗氧化、抗凋亡，减少子痫前期患者内皮细胞的损伤，提高内皮细胞的增殖能力及屏障功能，从而对子痫前期的发生、发展起预防和治疗的作用。

关键词: 染料木素, 　子痫前期, 　脐静脉内皮细胞, 　氧化应激, 　抗凋亡

Abstract: Objective　To study the effect of genistein on endothelial cell damage in preeclampsia. Methods　Human umbilical vein cells (HUVECs) were isolated from 20 normal pregnant women (control group) and 40 preeclampsia patients. Half of cells from the preeclampsia patients were treated with genistein (PE+Gen group) and the other half were untreated (PE group). HUVEC barrier function and proliferation were investigated using bovine serum albumin and MTT assays, respectively. Markers for oxidative stress and apoptosis were also assessed. 　Results　Compared to the PE group, HUVEC barrier function, proliferation, glutathione peroxidase activity, and Bcl-2 gene and protein expression were significantly increased in the genistein treatment group (PE+Gen), whereas 8-hydroxy-2’-deoxyguanosine levels and Bax gene and protein expression were significantly decreased (P<0.01). There was no significant difference between PE+Gen and control groups.　Conclusion　Genistein reduces endothelial cell damage possibly through antioxidant and anti-apoptotic mechanisms, and it may be useful in preeclampsia prevention and treatment.

Key words: , Genistein, Preeclampsia, 　Human umbilical vein endothelial cells, 　Oxidative stress, Anti-apoptosis

中图分类号:

R765.21

刘黎星, 闵羡慧, 张敏, 侯赛榕. Genistein对子痫前期患者离体脐静脉内皮细胞的保护作用及其机制研究#br#[J]. 中国临床解剖学杂志, 2019, 37(3): 261-266.

LIU Li-xing , MIN Xian-hui , ZHANG Min, HOU Sai-rong. Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms[J]. Chinese Journal of Clinical Anatomy, 2019, 37(3): 261-266.

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Genistein对子痫前期患者离体脐静脉内皮细胞的保护作用及其机制研究#br#

Effect of genistein on the number and function of HUVECs in preeclampsia patients and its mechanisms

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