NF-κB在变应性鼻炎发病中的调节作用

doi:10.13418/j.issn.1001-165x.2019.04.012

中国临床解剖学杂志 ›› 2019, Vol. 37 ›› Issue (4): 421-424.doi: 10.13418/j.issn.1001-165x.2019.04.012

NF-κB在变应性鼻炎发病中的调节作用

张敏，　吴聃，　刘黎星，　侯赛榕，　蔡丽希

莆田学院基础医学院解剖生理学教研室，福建莆田 351100

收稿日期:2019-03-13 出版日期:2019-07-25 发布日期:2019-08-01
作者简介:张敏（1969-），男，福建莆田人，博士，教授，研究方向：变应性疾病发病机制及干预治疗，Tel：（0564）2768812，E-mail：zm5995@sina.com
基金资助:
莆田学院育苗基金资助课题（2014058；2016111）；国家自然青年基金（81502360）

Role of NF-κB in pathogenesis of allergic rhinitis

ZHANG Min, WU Dan, LIU Li-xing, HOU Sai-rong, CAI Li-xi

Department of Anatomy and Physiology, Basic Medical College of Putian University, Putian 351100, Fujian Province, China

Received:2019-03-13 Online:2019-07-25 Published:2019-08-01

摘要/Abstract

摘要： 目的　探讨变应性鼻炎（allergic rhinitis，AR）病理过程中TLR-NF-κB信号传导通路的调节机制及核因子-κB（NF-κB）对嗜酸性粒细胞聚集的调节作用。方法　50只大鼠随机分成A组（对照组）、B组（AR组）、C组（AR+PGN）、D组（AR+PGN+Pam3CSK4）、E组（AR+PGN+BEL），应用卵清蛋白建造AR模型，HE染色观察鼻黏膜形态改变并计数炎症细胞浸润数，ELISA法检测鼻腔清洗液IFN-γ、IL-4、嗜酸性粒细胞主要碱性蛋白（MBP）及IgE的含量，Real-Time PCR及Western blot法检测Toll样受体2（TLR2）、NF-κB的表达情况。结果 B组变应性损伤明显，鼻黏膜嗜酸性粒细胞浸润数多于其他组，IL-4、MBP及IgE的表达较A组增高（P<0.05）；C组鼻黏膜以中性粒细胞浸润为主，IFN-γ、TLR2及NF-κB的表达较B组增高（P<0.05）；D、E组炎症细胞浸润数、IL-4、MBP及IgE的表达较B组降低（P<0.05）。结论　调控TLR-NF-κB传导通路的表达即抑制NF-κB的过度表达，有利于调节变应性鼻炎病理过程中嗜酸性粒细胞的增值聚集。

关键词: 核因子-κB, 　Toll样受体, 　肽聚糖, 　嗜酸性粒细胞, 　变应性鼻炎

Abstract: Objective　To experimentally investigate a potential mechanism of the TLR-NF-κB signaling pathway in the pathogenesis of allergic rhinitis (AR), and the role of NF-κB in eosinophil aggregation.　Methods　A total of 50 rats were randomly divided into five groups with A (control group), B (AR group), C (group of AR+PGN), D (group of AR+PGN+Pam3CSK4), and E (group of AR +PGN+BEL). AR model was established with ovalbumin. Hematoxylin and eosin (H&E) staining was used to detect the morphologic change of nasal mucosa and count the number of infiltrated inflammatory cell. The contents of IFN-γ, IL-4, MBP and IgE in nasal cleanser were detected by ELISA. Expression of NF-κB and TLR2 was measured by Real-Time polymerase chain reaction (PCR) and Western blot.　Results　Obvious allergic injury was observed in AR rats (B group). The number of infiltrated eosinophils in nasal mucosa in group B was higher than in other groups. The expressions of IL-4, MBP and IgE in group B were higher than in group A (P＜0.05). Neutrophil infiltration was predominant in nasal mucosa of group C, and the expression of IFN-γ, TLR2 and NF-κB was higher in group B (P＜0.05). Inflammatory cell infiltration, IL-4, MBP and IgE expression in group D and E were lower than in group B (P＜0.05).　Conclusions　Regulating the expression of TLR-NF-κB pathway and inhibiting the over-expression of NF-κB is beneficial to regulating the aggregation of eosinophils in the pathogenesis of allergic rhinitis.

Key words: , NF-κB, 　 Toll-like receptor, 　 PGN, 　Eosinophils, 　Allergic rhinitis

中图分类号:

R765
R392.3

张敏, 吴聃, 刘黎星, 侯赛榕, 蔡丽希 . NF-κB在变应性鼻炎发病中的调节作用[J]. 中国临床解剖学杂志, 2019, 37(4): 421-424.

ZHANG Min, WU Dan, LIU Li-xing, HOU Sai-rong, CAI Li-xi . Role of NF-κB in pathogenesis of allergic rhinitis[J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 421-424.

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NF-κB在变应性鼻炎发病中的调节作用

Role of NF-κB in pathogenesis of allergic rhinitis

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