关键词: 橙汁提取物, 　银纳米颗粒, 　生物相容性, 　抗菌性, 　绿色化学工程

Abstract: Objective　To explore the conditions for maximizing the yield of silver nanoparticles synthesized by extractive orange juice, and to evaluate the biocompatibility of such silver nanoparticles. Methods　The yield of silver nanoparticles was measured under different pH values, temperature and reaction time, and the optimal reaction conditions were explored. The biocompatibility and antibacterial properties of silver nanoparticles were evaluated by CCK-8 kit, scanning electron microscopy and antibacterial experiments. 　Results 　When the pH value was 8.0, temperature was 0 ℃, reaction time was 4 h, the yield of silver nanoparticles was highest. The synthesized silver nanoparticles were spherical or ellipsoidal, with an average particle size of 15.54 nm and a zeta potential of -34.46, which was well dispersed. Silver nanoparticles with a concentration between 6.25-50 μg/ml had no toxic effects on rat skin fibroblasts and red blood cells. Silver nanoparticles at 12.5 μg/ml and above had significant inhibitory effects on E. coli, and silver nanoparticles at 100 μg/ml and below did not significantly inhibit S. aureus.　Conclusions　The silver nanoparticles synthesized by the extractive orange juice at pH=8.0, temperature of 0 ℃ and reaction time of 4 h have the highest yield. These silver nanoparticles have small particle size, uniform dispersion, and are not easy to agglomerate, and have good biocompatibility and antibacterial properties. 

Key words: Extractive orange juice, 　Silver nanoparticles, 　Biocompatibility, 　Antibacterial properties, Green chemical engineering