目的 研究虫草素对大脑中动脉局灶性脑缺血模型大鼠氧化应激指标和脑组织Caspase-3和p53表达的影响。 方法 首先，给药组大鼠每天分别腹腔注射虫草素5、10、20 mg/kg，连续10 d；然后，采用改良Zea Longa线栓法制备大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）模型；造模24 h后，盲法进行神经功能评分，称重法检测脑含水量，HE染色观察脑组织病理损伤，Tunnel染色检测脑细胞凋亡，RT-PCR检测Bcl-2、Bax、Caspase-3和p53 mRNA表达，Western blotting检测Bcl-2、Bax、Caspase-3和p53蛋白表达，试剂盒检测SOD，MDA，GSH水平。 结果 给药组与MCAO组相比，神经功能评分显著降低，脑含水量显著减少，细胞损伤减轻，细胞凋亡率显著减少，Bax mRNA及蛋白表达显著下调，Bcl-2 mRNA及蛋白表达显著上调，MDA含量显著下降，SOD和GSH含量显著上升，Caspase-3和p53 mRNA及蛋白表达显著下调，且这些效果随着虫草素给药量的增加更加显著。 结论 虫草素能够缓解大脑中动脉局灶性脑缺血引起的神经功能障碍和降低脑缺血引起的脑含水量升高，并能抑制大脑中动脉局灶性脑缺血模型大鼠氧化应激和细胞凋亡，从而减缓大脑中动脉局灶性脑缺血造成的损伤。
Abstract
Objective To investigate the effects of cordycepin on oxidative stress and expression of caspase-3 and P53 in brain tissue of rats with middle cerebral artery occlusion (MCAO). Methods First, rats in the drug-administered group were intraperitoneally injected with 5, 10, and 20 mg/kg of cordycepin for 10 days. Then, the middle cerebral artery occlusion model was prepared by modified Zea Longa suture method. The neurological function was scored by blind method after the MCAO was prepared for 24h. The brain water content was detected by weighing method. The pathological damage of brain tissue was observed by HE staining and Tunnel staining was used to detect the apoptosis of brain cell. The expression of Bcl-2, Bax, Caspase-3 and p53 mRNA was detected by RT-PCR. The protein expression of Bcl-2, Bax and Caspase were detected by Western blotting. The expression of SOD, MDA, GSH levels were detected by kit. Results Compared with the MCAO group, the neurological function score, the brain water content, the cell damage and the apoptosis rate of the drug-administered group significantly reduced. The Bax mRNA and protein expression, the expression of Caspase-3 and p53 mRNA and protein expression significantly down-regulated, and the Bcl-2 mRNA and protein expression significantly up-regulated. The content of MDA decreased, the content of SOD and GSH increased significantly. With the increasing of cordycepin, the effects were much more effective. Conclusions Cordycepin can relieve neurological dysfunction caused by focal cerebral ischemia in the middle cerebral artery and reduce brain water content caused by cerebral ischemia, and can also inhibit apoptosis and oxidative stress in rats with focal cerebral ischemia, which slows the damage caused by focal cerebral ischemia in the middle cerebral artery.
关键词
虫草素 /
大脑中动脉局灶性脑缺血 /
氧化应激 /
caspase-3 /
P53
Key words
Cordycepin; /
/
Middle cerebral artery focal cerebral ischemia; /
/
Oxidative stress; /
/
Caspase-3; /
/
P53
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基金
河南省医学科技攻关计划项目（201602168）
京ICP备08002354号-3
