左卡尼汀经β-catenin信号通路保护环磷酰胺所致大鼠睾丸损伤

doi:10.13418/j.issn.1001-165x.2020.03.009

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (3): 283-288.doi: 10.13418/j.issn.1001-165x.2020.03.009

左卡尼汀经β-catenin信号通路保护环磷酰胺所致大鼠睾丸损伤

曾鹏¹，　祁蕊¹，　王锦¹，　孙潇¹，　代佑果²，　张鹏飞¹，　杨力¹，　郭泽云¹

昆明医科大学　1.人体解剖学与组织胚胎学系，　2.临床肿瘤学院，昆明 650000

收稿日期:2019-05-29 出版日期:2020-05-25 发布日期:2020-06-02
通讯作者: 郭泽云，E-mail：guozeyun1@163.com；杨力，E-mail：yanglikm@163.com
作者简介:曾鹏（1992-），男，四川德阳人，在读研究生，主要从事生殖方面的研究，E-mail：zengpengwd@163.com
基金资助:
云南省科技厅联合专项（2018FE001-015）；昆明医科大学研究生创新基金（2019S069）

Protective effects of L-carnitine on testicular injury induced by cyclophosphamide in rats through β-catenin signaling

ZENG Peng¹，QI Rui¹，WANG Jin¹，SUN Xiao¹，DAI You-guo²，ZHANG Peng-fei¹，YANG Li¹，GUO Ze-yun¹

1.Department of Human Anatomy and Histology and Embryology, Kunming Medical University, Kunming 650500, China; 2.College of Clinical Oncology, Kunming Medical University, Kuming 650500, China

Received:2019-05-29 Online:2020-05-25 Published:2020-06-02

摘要/Abstract

摘要： 目的　探讨左卡尼汀（L-carnitine，LC）对环磷酰胺（cyclophosphamide，CP）所致大鼠睾丸损伤的保护作用及其机制。方法　将18只雄性大鼠随机分为3组：生理盐水（NS）组，CP组，CP+LC组，腹腔注射给药。观测大鼠体重、精神等一般情况及睾丸重量，采用HE染色检测睾丸损伤情况，免疫组化和Western blot法检测睾丸内β-catenin的表达。结果　CP组大鼠睾丸重量低于NS组（P<0.05），CP+LC组大鼠睾丸重量比CP组有所恢复（P<0.05）；HE染色显示CP组和CP+LC组比NS组生精细胞层数减少（P<0.05），但CP+LC组较CP组生精细胞层数增多（P<0.05）；免疫组化和Western blot均显示CP组β-catenin的表达量高于NS组（P<0.05），CP+LC组β-catenin表达量低于CP组（P<0.05）。结论　左卡尼汀可下调β-catenin信号进而对环磷酰胺所致大鼠睾丸损伤起到保护作用，可能对抗瘤药引起的男性生育障碍具有潜在的治疗意义。

关键词: 左卡尼汀, 　环磷酰胺, 　睾丸, 　β-catenin

Abstract: Objective　To investigate the protective effects and possible mechanisms of L-carnitine (LC) on cyclophosphamide (CP) induced testicular injury in rats.　Methods　Eighteen male rats were randomly divided into three groups and received intraperitoneal injection of appropriate drug（a Normal saline group, a CP group, a CP+LC group）. General conditions, weight and testicular weight of rats were recorded. The testicular injury condition was detected by HE staining, and the expression of β-catenin in testis was detected by immunohistochemistry and western blotting.　Results　The testicular weight in the CP group was lower than that of the NS group（P<0.05）. While the testicular weight recovered in the CP+LC group（P<0.05）. HE staining result showed that the number of spermatogenic cell layers in CP group decreased significantly than that of the NS group（P<0.05）, but the number of spermatogenic cell layers of the CP+LC group was higher than that of the CP group（P<0.05）. Immunohistochemistry and Western blot result showed that the expression of β-catenin in the CP group was higher than that of the NS group（P<0.05）while the expression of β-catenin in the CP+LC group was lower than that of the CP group（P<0.05）. Conclusions L-carnitine can protect testicular injury in rats which induced by Cyclophosphamide through down-regulated β-catenin signaling, which makes a potential therapeutic significance for male fertility disorder caused by antitumor drugs.

Key words: , L-carnitine, 　Cyclophosphamide, 　Testis, 　β-catenin

中图分类号:

R453.9

曾鹏, 祁蕊, 王锦, 孙潇, 代佑果, 张鹏飞, 杨力, 郭泽云. 左卡尼汀经β-catenin信号通路保护环磷酰胺所致大鼠睾丸损伤[J]. 中国临床解剖学杂志, 2020, 38(3): 283-288.

ZENG Peng, QI Rui, WANG Jin, SUN Xiao, DAI You-guo, ZHANG Peng-fei, YANG Li, GUO Ze-yun. Protective effects of L-carnitine on testicular injury induced by cyclophosphamide in rats through β-catenin signaling[J]. Chinese Journal of Clinical Anatomy, 2020, 38(3): 283-288.

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左卡尼汀经β-catenin信号通路保护环磷酰胺所致大鼠睾丸损伤

Protective effects of L-carnitine on testicular injury induced by cyclophosphamide in rats through β-catenin signaling

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