小剂量超短波对脊髓损伤功能恢复及对血清iNOS、TNF-α、VEGF、BDNF表达的影响

doi:10.13418/j.issn.1001-165x.2020.05.019

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (5): 588-592.doi: 10.13418/j.issn.1001-165x.2020.05.019

小剂量超短波对脊髓损伤功能恢复及对血清iNOS、TNF-α、VEGF、BDNF表达的影响

梁爱萍，　李文辉

遵义医科大学第五附属珠海医院康复医学科，广东珠海 519100

收稿日期:2019-07-01 出版日期:2020-09-25 发布日期:2020-10-21
作者简介:梁爱萍（1972-），女，主任医师，研究方向：神经康复，肌骨康复，Tel：15913236878, E-mail: 3516129269@qq.com

Effect of low-dose ultrashort wave on functional recovery and expression of serum iNOS, TNF-α, VEGF, and BDNF in patients with spinal cord injury

LIANG Ai-ping， LI Wen-hui

Department of Rehabilitation Medicine, the Fifth Affiliated Zhuhai Hospital of Zunyi Medical University, Zhuhai 519100, Guangdong Province, China

Received:2019-07-01 Online:2020-09-25 Published:2020-10-21

摘要/Abstract

摘要： 目的　探讨小剂量超短波对脊髓损伤患者功能恢复效果及对血清诱导型一氧化氮合酶（iNOS）、肿瘤坏死因子-α（TNF-α）、血管内皮生长因子（VEGF）、脑源性神经营养因子（BDNF）表达的影响。方法　收集60例脊髓损伤患者，随机分为治疗组与对照组，每组30例。对照组给予基础康复治疗，治疗组加用小剂量超短波治疗，共治疗3个疗程。评估疗效并分析影响脊髓功能恢复的因素。结果　治疗组总有效率高于对照组（P<0.05）；康复治疗后，治疗组ASIA量表运动功能、感觉功能评分高于对照组（P<0.05）；血清VEGF、BDNF表达含量高于对照组（P<0.05），iNOS、TNF-α表达含量低于对照组（P<0.05）；小剂量超短波治疗是脊髓损伤患者预后的保护因素（P<0.05）。结论　小剂量超短波辅助治疗可明显促进患者脊髓功能恢复，其机制可能与下调血清iNOS、TNF-α表达，上调VEGF、BDNF表达有关。

关键词: 超短波, 　脊髓损伤, 　脊髓功能, 　iNOS, TNF-α

Abstract: Objective　To investigate the effect of low-dose ultrashort wave on functional recovery and serum inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), and brain-derived neurotrophic factor (BDNF) expression.　Methods　Sixty patients with spinal cord injury were enrolled and randomly divided into a treatment group and a control group, with 30 cases in each group. The control group was given basic rehabilitation treatment, and the treatment group was treated with small dose ultrashort wave therapy for a total of 3 courses of treatment. Efficacy was assessed and factors affecting spinal function recovery were analyzed.　Results　The total effective rate of the treatment group was higher than that of the control group (P<0.05). After rehabilitation treatment, the motor function and sensory function scores of the ASIA scale in the treatment group were higher than those in the control group (P<0.05). The levels of serum VEGF and BDNF in the treatment group were higher than those in the control group (P<0.05). The expression levels of iNOS and TNF-α in the treatment group were lower than those in the control group (P<0.05). Low dose ultrashort wave therapy was a protective factor for prognosis of patients with spinal cord injury (P<0.05).　Conclusions　Low-dose ultrashort wave adjuvant therapy can significantly promote the recovery of spinal cord function in patients, which may be related to down-regulation of serum iNOS, TNF-α expression and up-regulation of VEGF and BDNF expression.

Key words: , Ultrashort wave；　Spinal cord injury；　Spinal cord function；　iNOS；　TNF-α

中图分类号:

R493

梁爱萍, 李文辉. 小剂量超短波对脊髓损伤功能恢复及对血清iNOS、TNF-α、VEGF、BDNF表达的影响[J]. 中国临床解剖学杂志, 2020, 38(5): 588-592.

LIANG Ai-ping, LI Wen-hui . Effect of low-dose ultrashort wave on functional recovery and expression of serum iNOS, TNF-α, VEGF, and BDNF in patients with spinal cord injury[J]. Chinese Journal of Clinical Anatomy, 2020, 38(5): 588-592.

参考文献 15

[1]	Spinal cord injury (SCI) 2016 facts and figures at a glance[J]. J Spinal Cord Med, 2016, 39(4): 493-494.
[2]	吴猛, 李雄锋, 吴玮, 等. 神经节苷脂联合康复训练对脊髓损伤患者炎性因子及神经功能恢复的影响[J]. 中国临床药理学杂志, 2016, 32(12):1081-1084.
[3]	李敏, 杨孝, 孙年怡, 等. 脊髓损伤中TNF-α诱导细胞凋亡和坏死性凋亡的研究进展[J]. 广东医学, 2018, 39(18):2841-2844.
[4]	孙师, 万峪岑, 赵利娜, 等. 骨髓间充质干细胞联合超短波对大鼠脊髓损伤后早期AQP-4和GAP-43的影响[J]. 中国康复医学杂志, 2016,31(6): 625-631.
[5]	敖强, 吕德成, 姜长明, 等. 脊髓损伤后细胞凋亡及iNOS的表达[J]. 中国康复理论与实践, 2003, 9(1):38-41.
[6]	美国脊髓损伤协会, 国际脊髓损伤学会, 李建军, 等. 脊髓损伤神经学分类国际标准(2011年修订)[J]. 中国康复理论与实践, 2011, 17(10):963-971.
[7]	Lammertse D, Tuszynski MH, Steeves JD, et al. Guidelines for the conduct of clinical trials for spinal cord injury as developed by the ICCP panel: clinical trial design. [J]. Spinal Cord, 2007, 45(3):232-242.
[8]	张士杰. 临床脊柱外科学[M]. 北京: 科学技术文献出版社, 2008:39-42.
[9]	Nam KY, Kim HJ, Kwon BS, et al. Robot-assisted gait training (Lokomat) improves walking function and activity in people with spinal cord injury: a systematic review.[J]. J Neuroeng Rehabil, 2017, 14(1):24.
[10]	万峪岑, 孙师, 赵利娜, 等. 小剂量超短波治疗对大鼠脊髓损伤后炎症反应及水肿的影响[J]. 中国康复理论与实践, 2016, 22(2):150-155.
[11]	周刚, 王佳君. 高频电疗对急性脊髓损伤大鼠神经功能恢复和BDNF-TrkB表达的影响[J]. 颈腰痛杂志, 2018, 39(6): 694-697.
[12]	万峪岑, 孙师, 冯智萍, 等. 小剂量超短波治疗对大鼠脊髓损伤后VEGF和BDNF的影响[J]. 中国康复医学杂志, 2018, 33(8): 891-895.
[13]	尹振春, 董英海, 朱菁, 等. He-Ne激光对急性脊髓损伤总NOS、iNOS和ET-1的影响[J]. 应用激光, 2005, 25(3):214-216.
[14]	祝志强, 李佳, 孙晴, 等. 虎杖苷对急性脊髓损伤大鼠氧化应激反应和炎症反应影响[J]. 临床军医杂志, 2018, 46(4):435-438.
[15]	张军卫, 唐和虎, 李想, 等. Denis,Magerl和AO2013三种胸腰椎损伤分类方法的对比分析[J]. 中华骨与关节外科杂志, 2017, 10(4):312-316.

小剂量超短波对脊髓损伤功能恢复及对血清iNOS、TNF-α、VEGF、BDNF表达的影响

Effect of low-dose ultrashort wave on functional recovery and expression of serum iNOS, TNF-α, VEGF, and BDNF in patients with spinal cord injury

可视化

摘要/Abstract

引用本文

使用本文

参考文献 15

相关文章 3

Metrics

本文评价

推荐阅读 0

[1]	曹瀛心, 迟晓晨, 包翠芬, 李婷钰, 刘霞. 人参皂苷Rg1通过TNF-α/TNFR1/RIPKs通路调控急性肾损伤致急性肺损伤的保护机制研究[J]. 中国临床解剖学杂志, 2023, 41(4): 409-415.
[2]	王雨, 陈传奇, 王文晟, 喻保军, 张义, 李丹, 陈龙菊. 葛根素对臂丛神经根性撕脱伤脊髓前角iNOS、CGRP蛋白表达及PI3K/Akt信号通路的影响[J]. 中国临床解剖学杂志, 2019, 37(5): 517-522.
[3]	屈丽华, 屈姣华, 龙治峰, 李美香, 石金凤, 罗红梅, 莫中成. 生后不同发育阶段大鼠卵巢内eNOS和iNOS的表达[J]. 中国临床解剖学杂志, 2012, 30(2): 203-208.