离断大鼠迷走神经肝支对血糖血脂水平的影响及其与mTOR表达水平的关系

doi:10.13418/j.issn.1001-165x.2020.06.008

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (6): 661-667.doi: 10.13418/j.issn.1001-165x.2020.06.008

离断大鼠迷走神经肝支对血糖血脂水平的影响及其与mTOR表达水平的关系

曹娜¹，　程子娟¹，　李肇蕤¹，　刘漫君²，　朱琳³，　夏春波¹

桂林医学院 1. 解剖学教研室，　2. 附属医院新生儿科，　3. 附属医院病理科，广西桂林 541001

收稿日期:2019-11-12 出版日期:2020-11-25 发布日期:2020-12-08
通讯作者: 夏春波，教授，E-mail：xiachunbo910@163.com
作者简介:曹娜（1992-），女，安徽宿州人，硕士，主要研究方向为能量代谢的神经调节机制，E-mail：2608500177@qq.com
基金资助:
国家自然科学基金（81760159，81960163）；广西自然科学基金（2017GXNSFAA198307，2018GXNSFBA138029）

Effects of severed hepatic branch of vagus nerve on blood glucose and lipid levels and its relationship with mTOR expression level in rats

CAO Na¹, CHENG Zi-juan¹, LI Zhao-rui¹, LIU Man-jun², ZHU Lin³, XIA Chun-bo¹

1. Department of Anatomy, Guilin Medical College, Guilin 541004, China; 2. Department of Neonatology, Affiliated Hospital, Guilin Medical College, Guilin 541004, China; 3. Department of Pathology, Affiliated Hospital, Guilin Medical College, Guilin 541004, Guangxi Province, China

Received:2019-11-12 Online:2020-11-25 Published:2020-12-08

摘要/Abstract

摘要： 目的　探讨离断大鼠迷走神经肝支对血糖、血脂水平的影响及其与mTOR表达水平的关系，为糖脂代谢神经调节机制研究提供实验依据。方法　建立离断迷走神经肝支大鼠模型作为实验组，另设迷走神经肝支探查的假手术组和不作任何处理的对照组，术后每两周检测大鼠血糖、血脂、胰岛素和皮质醇水平、肝和骨骼肌组织的糖原含量以及mTOR的表达水平。结果　实验组大鼠与对照组大鼠相比，术后2周胰岛素水平升高、肝糖原含量增多、血糖水平降低（P<0.05）；术后6周，肝、骨骼肌组织mTOR表达增多、肝糖原及骨骼肌糖原含量降低、血糖水平增高、甘油三酯水平增高，高密度脂蛋白水平降低（P<0.05）。假手术组和对照组大鼠各项指标各时间点无明显差异（P>0.05）。结论　离断大鼠迷走神经肝支可导致糖脂代谢以及糖原含量异常，其机制可能与胰岛素水平变化以及肝、骨骼肌mTOR表达水平升高有关。

关键词: 迷走神经肝支, 　大鼠, 　代谢, 　糖原, 　mTOR

Abstract: Objective　To investigate the effect of cutting vagus nerve hepatic branch on blood glucose and lipid levels and its relationship with mTOR expression level in rats, to provide experimental evidence for the study of neuromodulation mechanism of glycolipid metabolism.　Methods　Rat model of hepatic branch of vagus nerve transection was established by surgical method (n=15), and the sham operation group (n=15) with vagus hepatic branch exploration and the control group without any treatment (n=15) were set up. The levels of blood glucose, blood lipid, insulin and cortisol, glycogen content in liver and skeletal muscle and the expression of mTOR were measured every two weeks after operation. Results Compared with the control group, two weeks after operation, the blood glucose level of the experimental group decreased, the content of liver glycogen increased and the level of insulin increased (P<0.05). Six weeks after operation, the level of blood glucose increased, the content of glycogen in liver and skeletal muscle decreased, the level of triglyceride increased, the level of HDL decreased, and the expression of mTOR in liver and skeletal muscle increased (P<0.05). There was no significant difference between the sham group and the control group at each time point (P>0.05). Conclusions The transetion of rat vagus nerve hepatic branch can lead to abnormal glucose and lipid metabolism and glycogen content, which may be related to the changes in insulin level and increased expression of mTOR in liver and skeletal muscle.

Key words: Hepatic branch of vagus nerve, 　Rat;　 Metabolism, 　Glycogen, 　mTOR

中图分类号:

R332

曹娜, 程子娟, 李肇蕤, 刘漫君, 朱琳, 夏春波. 离断大鼠迷走神经肝支对血糖血脂水平的影响及其与mTOR表达水平的关系[J]. 中国临床解剖学杂志, 2020, 38(6): 661-667.

CAO Na, CHENG Zi-juan, LI Zhao-rui, LIU Man-jun, ZHU Lin, XIA Chun-bo. Effects of severed hepatic branch of vagus nerve on blood glucose and lipid levels and its relationship with mTOR expression level in rats[J]. Chinese Journal of Clinical Anatomy, 2020, 38(6): 661-667.

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离断大鼠迷走神经肝支对血糖血脂水平的影响及其与mTOR表达水平的关系

Effects of severed hepatic branch of vagus nerve on blood glucose and lipid levels and its relationship with mTOR expression level in rats

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