蛋白激酶WEE1在胃癌组织中的表达及其临床意义

doi:10.13418/j.issn.1001-165x.2020.06.011

中国临床解剖学杂志 ›› 2020, Vol. 38 ›› Issue (6): 680-684.doi: 10.13418/j.issn.1001-165x.2020.06.011

蛋白激酶WEE1在胃癌组织中的表达及其临床意义

李菁¹，　金玉²，　李启阳¹，　许英卓¹，　刘超²，　肖建英¹

锦州医科大学基础医学院　1.生物化学与分子生物学教研室，　2.遗传学教研室，辽宁锦州 121001

收稿日期:2020-01-29 出版日期:2020-11-25 发布日期:2020-12-08
通讯作者: 肖建英，教授，硕士研究生导师，E-mail：lyxiaojy@163.com
作者简介:李菁（1995-），女，辽宁沈阳人，在读硕士，主要从事分子生殖和分子肿瘤方面的研究，E-mail：lijingmelody@163.com
基金资助:
国家自然资金资助课题（81270698，31371173，81401199）；辽宁省科技厅科学技术计划项目资助课题（2015020697）；辽宁省教育厅高等学校创新人才项目资助课题（LR2016075）

Expression of protein kinase WEE1 in gastric cancer tissue and its clinical significance

LI Jing¹, JIN Yu², LI Qi-yang¹, XU Ying-zhuo¹, LIU Chao², XIAO Jian-ying¹

1.Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121001,China; 2. Department of Genetics, School of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121001,China

Received:2020-01-29 Online:2020-11-25 Published:2020-12-08

摘要/Abstract

摘要： 目的　探讨蛋白激酶WEE1在胃癌组织中的表达及其与胃癌临床指标的相关性，并阐述其在胃癌发生发展中的作用。方法　使用Oncomine数据库分析WEE1在胃腺癌和胃黏膜组织中的表达差异。选取100例胃腺癌和80例癌旁组织标本，通过免疫组织化学法和组织芯片技术检测WEE1蛋白在胃癌和癌旁组织中的表达水平。对免疫组织化学结果中染色的比例以及染色程度进行半定量分析，对半定量分析结果与胃癌临床指标进行相关性分析。使用RT-PCR和Western blotting检测WEE1在胃癌和癌旁组织中的mRNA和蛋白表达水平。结果　WEE1在100例胃癌患者中的阳性率为86%，高表达86例，低表达14例。通过Oncomine数据库分析，蛋白激酶WEE1在胃腺癌中的表达明显高于胃黏膜组织中的表达（P<0.01）。免疫组化、RT-PCR和Western blotting均显示，WEE1在胃癌组织中的表达明显高于癌旁组织（P<0.01）；免疫组化还显示WEE1的表达量随着胃癌组织分期的增加而增加（P<0.01）。结论　蛋白激酶WEE1在胃癌组织中高表达，提示其可作为胃癌治疗靶点之一。

关键词: 蛋白激酶WEE1, 　胃癌, 　免疫组织化学, 　组织芯片, 　免疫印迹, 　逆转录-聚合酶链反应

Abstract: Objective　To investigate the expression of protein kinase WEE1 in gastric cancer tissues and the association between its protein expression and clinical indicators of patients with gastric cancer, and to clarify its role in the occurrence and development of gastric cancer.　Methods　The Oncomine database was used to analyze the differential expression of WEE1 in gastric adenocarcinoma and gastric mucosa. Tissue microarray and immunohistochemistry were used to detect the expression of WEE1 protein using 100 cases of gastric adenocarcinoma and 80 cases of adjacent tissues. The proportion of staining in immunohistochemistry and the degree of staining were semi-quantitatively analyzed, and the correlation between semi-quantitative analysis and clinical indexes of gastric cancer patients was relevance analyzed. Furthermore, reverse transcription (RT)-polymerase chain reaction (PCR) and Western blotting were used to detect the mRNA and protein expression of WEE1 in gastric cancer cells and gastric mucosa cells.　Results　The positive rate of WEE1 in gastric cancer tissues was 86%, of which 14 cases were low expression and 86 cases were high expression of the 100 patients. Oncomine database analysis showed that the expression of WEE1 mRNA in mixed gastric adenocarcinoma was higher than that in gastric mucosa (P<0.01). The results of immunohistochemistry, RT-PCR and Western blotting showed that the expression of WEE1 in gastric cancer tissues was significantly higher than that in adjacent tissues (P<0.01). Moreover, the immunohistochemistry showed that the protein expression of WEE1 increased with the increasing clinical stage of gastric cancer (P<0.01). 　Conclusions Protein kinase WEE1 is highly expressed in gastric cancer tissues, suggesting that it may act as one of the therapeutic targets of gastric cancer.

Key words: Protein kinase WEE1, 　Gastric cancer, 　Immunohistochemistry, 　Tissue microarray, , Western blotting, , , RT-PCR

中图分类号:

R735.2

李菁, 金玉, 李启阳, 许英卓, 刘超, 肖建英. 蛋白激酶WEE1在胃癌组织中的表达及其临床意义[J]. 中国临床解剖学杂志, 2020, 38(6): 680-684.

LI Jing, JIN Yu, LI Qi-yang, XU Ying-zhuo, LIU Chao, XIAO Jian-ying. Expression of protein kinase WEE1 in gastric cancer tissue and its clinical significance[J]. Chinese Journal of Clinical Anatomy, 2020, 38(6): 680-684.

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蛋白激酶WEE1在胃癌组织中的表达及其临床意义

Expression of protein kinase WEE1 in gastric cancer tissue and its clinical significance

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