关键词: 瑞香素, 　反复自然流产, 　透射电镜, 　蜕膜组织

Abstract: Objective　To observe the ultrastructural changes of decidual tissues in recurrent spontaneous abortion (RSA) model mice after treated with E003.　Methods　The RSA model mice was established, the experiment was divided into the following four groups: a normal pregnancy control group, a RSA model group, a low dose E003 group and a high dose E003 group.  After 14 days of continuous gavage of E003, decidual tissues were taken. Light microscope was used to observe the vascular and cell morphology of decidual tissue, ultrastructure of decidual tissue.　Results　The uterus was dissected to calculate the absorption rate of the embryo. The results showed that the E003 treatment group (low-dose group and high-dose group) had lower embryo absorption rate than that of the RSA model group, and the difference was statistically significant (P<0.05).There was no significant difference in embryo absorption rate between the high-dose E003 group and the normal pregnancy group (P>0.05). Compared with the normal pregnancy group, the number of decidual cells in RSA model group significantly reduced. Compared with the model group, the decidua cells in the low dose group and the high dose group increased in number and were arranged neatly. Under electron microscope, the volume of deciduous cell in the RSA model group decreased compared with the normal pregnancy group, the space of cells was larger, vascular wall was incomplete, vascular endothelial cell structure was incomplete and the nucleus was fusiform. Compared with the model group, the intercellular space and volume of decidual cell volume in the E003 low-dose group increased. The vascular wall was more complete, the vascular endothelial cells were long fusiform, the vascular endothelial cell structure was more complete, and the intercellular space was slightly wider. The decidual cell tissue and blood vessel in the high-dose E003 group was close to the normal pregrancy group.    Conclusions    RSA model mice have the barrier of maternal-fetal interface angiogenesis, and E003 can repair the damaged cell structure in the decidua tissue of RSA model mice, improve the morphology of angiogenesis and organelles, which has a certain therapeutic effect on RSA model mice.

Key words: Daphnetin;　Recurrent spontaneous abortion;　Transmission electron microscope; ,  , Decidual tissue