颞叶外侧癫痫患者脑组织NHE1及凋亡相关蛋白的表达

doi:10.13418/j.issn.1001-165x.2021.04.013

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (4): 431-436.doi: 10.13418/j.issn.1001-165x.2021.04.013

颞叶外侧癫痫患者脑组织NHE1及凋亡相关蛋白的表达

吴旭玲¹，　董楝¹，　彭爽¹，　叶兰²，　徐祖才³，　冯占辉¹

1.贵州医科大学附属医院神经内科，贵阳 550004； 2.贵州医科大学基础医学院，贵阳 550004；
3.遵义医科大学附属医院神经内科，贵州遵义 563099

收稿日期:2020-02-28 出版日期:2021-07-25 发布日期:2021-07-25
通讯作者: 冯占辉，E-mail：h9450203@126.com
作者简介:吴旭玲（1989-），女，主治医师，研究方向：癫痫与认知障碍性疾病，E-mail：351756257@qq.com
基金资助:
国家自然科学基金（81860248，81960224）；中国抗癫痫协会癫痫科研基金（2018016）；贵阳市科技局及贵州医科大学联合基金项目（筑科合同[2017]30-32）

The expression of NHE1 and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy

Wu Xuling¹，Dong Lian¹，Peng Shuang¹， Ye Lan²， Xu Zucai³，Feng Zhanhui¹

1.Department of Neurology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China; 2.School of Basic Medicine, Guizhou Medical University, Guiyang 550004, China; 3.Department of Neurology, Affiliated hospital of Zunyi Medical University, Zunyi 563099, China

Received:2020-02-28 Online:2021-07-25 Published:2021-07-25

摘要/Abstract

摘要： 目的　检测颞叶外侧癫痫患者手术切除脑组织标本中钠氢交换体1（Na+/H+ exchanger-1，NHE1）的表达及凋亡相关蛋白的表达，初步探讨其致病机制。方法　收集16例颞叶外侧癫痫患者手术切除脑组织标本作为癫痫组，10例颞叶肿瘤和脑出血患者手术切除脑组织标本作为对照组，HE染色观察脑组织病理学变化，免疫组化染色法以及免疫荧光法检测NHE1表达特点，Western blot等方法半定量分析NHE1蛋白表达特点，并检测凋亡相关蛋白Bcl-2与Bax表达特点。结果　HE染色可见癫痫组颞叶组织中神经元明显减少，吞噬现象明显增多，小胶质细胞过度活跃，而对照组标本无类似表现；免疫荧光及免疫组化染色提示NHE1定位于神经元上，癫痫组NHE1染色较对照组增强（P<0.05）；癫痫组NHE1蛋白半定量表达较对照组增高（P<0.05）；凋亡相关蛋白检测显示癫痫组Bcl-2蛋白表达较对照组降低（P<0.05），Bax蛋白表达较对照组增高（P<0.05）。结论　NHE1与颞叶外侧癫痫的形成和发展密切相关，其机制可能是NHE1增高参与调控神经元凋亡。

关键词: 颞叶外侧癫痫, 　钠氢交换体1, 　凋亡, 　人脑组织

Abstract: Objective　To detect the expression of Na+/H+ exchanger-1 (NHE1) and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy after operation and explore the pathogenic mechanism preliminarily.　Methods　Sixteen samples from patients with lateral temporal lobe epilepsy were collected as an epilepsy group, 10 samples from patients with temporal lobe tumor and cerebral hemorrhage as a control group. The pathological changes of brain tissue were observed by HE staining. The expression of NHE1 were further detected by immunohistochemistry and immunofluorescence. Western blot and other method was used to semi-quantitatively analyze the expression characteristics of NHE1, Bcl-2 and Bax. The data were collected and analyzed by SPSS 24.0 software.　Results　HE staining showed that neurons decreased, phagocytosis increased and microglia was hyperactive in the temporal lobe of the epilepsy group. However, there was no similar expression in the control group. Immunofluorescence and immunohistochemical staining showed that NHE1 was located on neurons. NHE1 staining in the epilepsy group was significantly higher than that in the control group (P<0.05). The expression of NHE1 protein in the epilepsy group was significantly higher than that in the control group (P<0.05). The results of Bcl-2 and Bax showed that the expression of Bcl-2 in the epilepsy group was significantly lower than that in the control group (P<0.05), and the expression of Bax was significantly higher than that in the control group (P<0.05).　Conclusions　NHE1 is closely related to the formation and development of lateral temporal lobe epilepsy. The possible mechanism is closely related the increasing of NHE1 and neuron apoptosis.

Key words: Lateral temporal lobe epilepsy, 　NHE1, 　Apoptosis, 　Human brain tissue

中图分类号:

R742.1

吴旭玲, 董楝, 彭爽, 叶兰, 徐祖才, 冯占辉. 颞叶外侧癫痫患者脑组织NHE1及凋亡相关蛋白的表达[J]. 中国临床解剖学杂志, 2021, 39(4): 431-436.

Wu Xuling, Dong Lian, Peng Shuang, Ye Lan, Xu Zucai, Feng Zhanhui. The expression of NHE1 and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy[J]. Chinese Journal of Clinical Anatomy, 2021, 39(4): 431-436.

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颞叶外侧癫痫患者脑组织NHE1及凋亡相关蛋白的表达

The expression of NHE1 and apoptosis related protein in brain tissue of patients with lateral temporal lobe epilepsy

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