鸢尾素抑制JAK/STAT3通路促进炎症状态下的血管生成

doi:10.13418/j.issn.1001-165x.2021.06.012

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (6): 680-685.doi: 10.13418/j.issn.1001-165x.2021.06.012

鸢尾素抑制JAK/STAT3通路促进炎症状态下的血管生成

胡思涵^{1, 2}，　薛源^{1, 2}，　金叶盛²，　张元澍²，　施勤¹，　芮永军²

1.苏州大学附属第一医院苏州大学骨科研究所，江苏苏州 215006
2.苏州大学附属无锡第九人民医院，江苏无锡 214026

收稿日期:2021-03-01 出版日期:2021-11-25 发布日期:2021-12-01
通讯作者: 芮永军，主任医师，博士研究生导师，E-mail：ruiyj@hotmail.com；施勤，教授，博士研究生导师，E-mail：shiqin@suda.edu.cn
作者简介:胡思涵（1994-），女，江苏无锡人，硕士，住院医师，主要从事骨代谢相关研究，E-mail：sihanhu0628@126.com
基金资助:
江苏省自然科学基金（BK20181130）；太湖人才计划顶尖医学团队

Irisin promotes angiogenesis in inflammatory state by inhibiting the JAK/STAT3 pathway

Hu Sihan^1,2, Xue Yuan^1,2, Jin Yesheng², Zhang Yuanshu², Shi Qin¹, Rui Yongjun²

1.The First Affiliated Hospital of Soochow University, Institute of Orthopedics, Suzhou 215006, Jiangsu Province, China;2. Wuxi Ninth People’s Hospital affiliated to Soochow University, Wuxi 214026, Jiangsu Province, China

Received:2021-03-01 Online:2021-11-25 Published:2021-12-01

摘要/Abstract

摘要： 目的　研究急性炎症状态下鸢尾素对人脐静脉内皮细胞（human umbilical vein endothelial cell，HUVEC）血管生成的影响及其机制。方法　通过CCK-8检测不同浓度（0.1、1.0、5.0、10.0、20.0 ng/mL）的鸢尾素对HUVEC增殖的影响；用脂多糖诱导HUVEC急性炎症状态，通过划痕和成管实验，研究鸢尾素对细胞迁移及管样形成的影响，通过RT-PCR研究相关细胞因子IL-1β、IL-6、TNF-α、PDGF-BB的基因表达；Western blot检测JAK／STAT3信号通路中关键蛋白Erk1／2、Jak、Stat3的表达。结果　各浓度的重组鸢尾素均不影响HUVEC的增殖；在急性炎症状态下，鸢尾素可以促进HUVEC细胞迁移和管样形成，并且抑制HUVEC炎症因子IL-1β、IL-6、TNF-α基因的表达，增加组织修复相关因子PDGF-BB的基因表达；鸢尾素抑制LPS诱导的JAK／STAT3通路激活。结论　鸢尾素可以抑制JAK／STAT3信号通路，缓解LPS诱导的HUVEC急性炎症状态，促进血管新生。

关键词: 鸢尾素, 　缺血再灌注, 　HUVEC, 　LPS, 　JAK／STAT3

Abstract: Objective　To investigate the effect of irisin on angiogenesis in human umbilical vein endothelial cell (HUVEC) under acute inflammatory state and its regulatory mechanism.　Methods　 (1)The effects of different concentrations (0.1, 1.0, 5.0, 10.0, 20.0 ng/ml) of irisin on HUVEC proliferation were detected by CCK-8; (2) Acute inflammatory state of HUVEC was induced by LPS. The effect of irisin on cell migration and tubular formation was studied by scratch and tube forming experiments. The gene expression of related cytokines IL-1β, IL-6, TNF-α and PDGF-BB was studied by RT-PCR; (3) The expressions of proteins Erk1/2, Jak and Stat3 in the JAK/STAT3 signaling pathway were detected by Western Blot.　Results　 (1) The change of concentrations of recombinant irisin did not affect the proliferation of HUVEC; (2) Irisin promoted cell migration and tubular formation of HUVEC in the state of acute inflammation, and inhibited the expression of inflammatory factors IL-1β, IL-6 and TNF-α in HUVEC in the state of acute inflammation, and increased the gene expression of tissue repair factor PDGF-BB; (3) Irisin inhibited LPS-induced activation of the JAK/STAT3 pathway.　Conclusions　Irisin suppresses JAK/STAT3 signaling pathway, relieves acute inflammatory state of HUVEC induced by LPS, and promotes angiogenesis.

Key words: , Irisin, 　Ischemia reperfusion, 　HUVEC, 　LPS, 　JAK/STAT3

中图分类号:

R339.37

胡思涵, 薛源, 金叶盛, 张元澍, 施勤, 芮永军. 鸢尾素抑制JAK/STAT3通路促进炎症状态下的血管生成[J]. 中国临床解剖学杂志, 2021, 39(6): 680-685.

Hu Sihan, Xue Yuan, Jin Yesheng, Zhang Yuanshu, Shi Qin, Rui Yongjun . Irisin promotes angiogenesis in inflammatory state by inhibiting the JAK/STAT3 pathway[J]. Chinese Journal of Clinical Anatomy, 2021, 39(6): 680-685.

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鸢尾素抑制JAK/STAT3通路促进炎症状态下的血管生成

Irisin promotes angiogenesis in inflammatory state by inhibiting the JAK/STAT3 pathway

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