新型机械敏感离子通道Piezo1和TRPV4蛋白siRNA双沉默模式对骨关节炎大鼠模型的修复作用研究

doi:10.13418/j.issn.1001-165x.2021.06.014

中国临床解剖学杂志 ›› 2021, Vol. 39 ›› Issue (6): 692-699.doi: 10.13418/j.issn.1001-165x.2021.06.014

新型机械敏感离子通道Piezo1和TRPV4蛋白siRNA双沉默模式对骨关节炎大鼠模型的修复作用研究

周勇伟¹，章才华¹，李晓飞²，蔡迅梓¹

1.浙江大学医学院附属第二医院骨科，杭州浙江 310000； 2. 浙江大学医学院附属金华医院骨一科，金华浙江 321000

收稿日期:2020-12-30 出版日期:2021-11-25 发布日期:2021-12-01
通讯作者: 蔡迅梓，主任医师，硕士生导师，E-mail:2506053@zju.edu.cn
作者简介:周勇伟（1990-），男，浙江金华人，硕士，主治医生，研究方向：运动损伤及关节疾病诊治，E-mail：15958961645@163.com
基金资助:
浙江省自然科学基金（Q20H060058）；浙江省医药卫生科技项目（2020KY343）；金华市公益类项目（2019-4-002）

The repair effect of novel mechanical sensing ion channels Piezo1 and TRPV4 protein siRNA double silencing on osteoarthritis rat model

Zhou Yongwei¹, Zhang Caihua¹, Li Xiaofei², Cai Xunzi¹

1. Department of Orthopedics, the Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310000, China; 2. Department of Orthopedics, the Affiliated Jinhua Hospital of Zhejiang University Medical College, Jinhua 321000, China

Received:2020-12-30 Online:2021-11-25 Published:2021-12-01

摘要/Abstract

摘要： 目的　探究新型机械敏感离子通道Piezo1和TRPV4蛋白siRNA双沉默模式对骨关节炎大鼠模型的修复作用。方法　构建SD大鼠的OA动物模型，根据处理方案的不同，将SD大鼠分成4组，即空白对照组，siRNA-Piezo1组，siRNA-TRPV4组和双基因沉默组。通过HE染色和番红-固绿染色等方法，利用改良Minkin’s评分和OARSI评分进行评估。利用免疫组织化学染色和RT-PCR检测膝关节软骨组织中Aggrecan蛋白和Collagen II的表达。结果　HE染色和番红-固绿染色等结果显示，siRNA-Piezo1组和siRNA-TRPV4组膝关节软骨组织的评分要明显优于模型组，siRNA-Piezo1组和siRNA-TRPV4组（P<0.05）。免疫组织化学染色和RT-PCR结果显示，双基因沉默组中Aggrecan和Collagen II蛋白的表达量明显高于siRNA-Piezo1组和siRNA-TRPV4组（P<0.05）。结论　Piezo1和TRPV4蛋白siRNA双沉默模式可以促进Aggrecan和Collagen II的表达，进而对骨关节炎大鼠模型起到一定的修复作用。

关键词: , 机械激活离子通道；　骨关节炎；　动物模型；　修复

Abstract: Objective To explore the repair effect of siRNA double silencing of new mechanosensitive ion channels Piezo1 and TRPV4 on osteoarthritis rat model. Methods The osteoarthritis animal (OA) model of SD rats was established. According to the different treatment on rats, SD rats were divided into the following four groups: a blank control group, a siRNA-Piezo1 group, a siRNA-TRPV4 group and a double gene silencing group. HE staining and safranin solid green staining were used to evaluate the quality of the samples by modified Minkin's score and OARSI score. The expression of Aggrecan protein and Collagen II in articular cartilage were detected by immunohistochemical staining and RT-PCR. Results The result of the HE staining and safranin green staining showed that the scores of knee cartilage tissue in the siRNA-Piezo1 group and siRNA-TRPV4 group were significantly higher than that in the model group, the siRNA-piezo1 group and the siRNA-TRPV4 group (P<0.05). The results of immunohistochemical staining and RT-PCR showed that the expression of Aggrecan and Collagen II in the double gene silencing group was significantly higher than that in siRNA-piezo1 group and siRNA-TRPV4 group (P<0.05). Conclusions Piezo1 and TRPV4 protein siRNA double silencing mode can promote the expression of Aggrecan and Collagen II, and then play a certain role in the repair of osteoarthritis rat model.

Key words: Mechanical sensing ion channels; , , Osteoarthritis; , , Animal model; , , Repair

中图分类号:

R684.3

周勇伟, 章才华, 李晓飞, 蔡迅梓. 新型机械敏感离子通道Piezo1和TRPV4蛋白siRNA双沉默模式对骨关节炎大鼠模型的修复作用研究[J]. 中国临床解剖学杂志, 2021, 39(6): 692-699.

Zhou Yongwei, Zhang Caihua, Li Xiaofei, Cai Xunzi. The repair effect of novel mechanical sensing ion channels Piezo1 and TRPV4 protein siRNA double silencing on osteoarthritis rat model[J]. Chinese Journal of Clinical Anatomy, 2021, 39(6): 692-699.

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新型机械敏感离子通道Piezo1和TRPV4蛋白siRNA双沉默模式对骨关节炎大鼠模型的修复作用研究

The repair effect of novel mechanical sensing ion channels Piezo1 and TRPV4 protein siRNA double silencing on osteoarthritis rat model

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