CDMNP-PEG-CD磁纳米颗粒诱导的骨骼肌反应性分析

doi:10.13418/j.issn.1001-165x.2022.2.11

中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (2): 173-180.doi: 10.13418/j.issn.1001-165x.2022.2.11

CDMNP-PEG-CD磁纳米颗粒诱导的骨骼肌反应性分析

菅晓婷¹，　王涵¹，　黄静雯¹，　蓝海强¹，　廖钊宏¹，　柯渔²，　廖华¹*，　诸春敏³*

1.广东省组织构建与检测重点实验室，南方医科大学基础医学院解剖教研室，广州 510515； 2. 暨南大学生命科学
与技术学院生物医学工程系，广州 510515； 3. 兰州大学基础医学院人体解剖与组织胚胎教研室，兰州 730000

收稿日期:2022-02-15 出版日期:2022-03-25 发布日期:2022-04-12
通讯作者: 诸春敏，硕士，讲师，E-mail: zhuchunmin@lzu.edu.cn；廖华，教授，E-mail: hua-liao@163.com
作者简介:菅晓婷（1997-），女，山西人，硕士研究生，研究方向：骨骼肌损伤, Tel：18803508955，E-mail:jianxiaotingii@163.com
基金资助:
国家自然科学基金面上项目（32071181）；广东省自然科学基金面上项目（2019A1515011305）；广州市科技计划项目（202002030497）

Analysis of skeletal muscle reactivity induced by CDMNP-PEG-CD magnetic nanoparticles

Jian Xiaoting¹, Wan Han¹, Huang Jingwen¹, Lan Haiqiang¹, Liao Zhaohong¹, Ke Yu², Liao Hua¹*, Zhu Chunmin ³*

1. Guangdong Provincial Key Laboratory of Tissue Construction and Testing, Department of Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515; 2. Department of Biomedical Engineering, School of Life Science and Technology, Jinan University, Guangzhou 510515; 3. Department of Human Anatomy and Tissue and Embryology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000

Received:2022-02-15 Online:2022-03-25 Published:2022-04-12

摘要/Abstract

摘要： 目的　探讨SOMNP、CDMNP及CDMNP-PEG-CD磁性纳米颗粒置入小鼠骨骼肌所诱发的免疫反应性。方法　以磁性Fe3O4与SiO2复合制备SOMNP, 将聚乙二醇（PEG）、环糊精（β-CD）分子链接SOMNP合成CDMNP，进一步添加聚假单胞烷（polypseudorotaxanes）合成CDMNP-PEG-CD磁性纳米颗粒。分别将3种纳米颗粒置入B6小鼠腓肠肌。组化染色、免疫荧光及FACS分析，分别评估不同置入时段SOMNP、CDMNP和CDMNP-PEG-CD纳米颗粒所诱发的肌毒性及肌内炎性渗出特征。结果　SOMNP链接β-CD、PEG/β-CD及polypseudorotaxanes将限制纳米颗粒的肌内扩散，导致CDMNP和CDMNP-PEG-CD在肌内的滞留时间延长、材料邻近区肌细胞坏死、肌内单核/巨噬细胞聚集。较之CDMNP-PEG-CD， CDMNP纳米颗粒吸引更多T细胞进入肌组织。结论　SOMNP、CDMNP及CDMNP-PEG-CD纳米颗粒均为潜在的体内免疫诱导物。polypseudorotaxanes修饰赋予CDMNP-PEG-CD纳米颗粒较好的体内相容性。

关键词: 磁纳米颗粒；　CDMNP-PEG-CD；　SOMNP；　CDMNP；　免疫反应；　骨骼肌

Abstract: Objective　To investigate the immunoreactivity induced by SOMNP, CDMNP and CDMNP-PEG-CD magnetic nanoparticles implanted into mouse skeletal muscle.　Methods　SOMNP was prepared by compounding magnetic Fe3O4 and SiO2. Polyethylene glycol (PEG) and cyclodextrin (β-CD) molecules were linked to SOMNP to synthesize CDMNP, and polypseudorotaxanes were further added to synthesize CDMNP-PEG-CD magnetic nanoparticles. particles. Three kinds of nanoparticles were placed into the gastrocnemius muscle of B6 mice, respectively. Histochemical staining, immunofluorescence and FACS analysis were used to evaluate the characteristics of myotoxicity and intramuscular inflammatory exudation induced by SOMNP, CDMNP and CDMNP-PEG-CD nanoparticles at different implantation periods.　Results　Linking of SOMNP to β-CD, PEG/β-CD and polypseudorotaxanes will limit the intramuscular diffusion of nanoparticles, resulting in prolonged intramuscular residence time of CDMNP and CDMNP-PEG-CD, myocyte necrosis in the vicinity of the material, intramuscular mononuclear/Macrophage aggregation. Compared with CDMNP-PEG-CD, CDMNP nanoparticles attracted more T cells into muscle tissue. Conclusion　SOMNP, CDMNP and CDMNP-PEG-CD nanoparticles are all potential immune inducers in vivo. The modification of polypseudorotaxanes endowed CDMNP-PEG-CD nanoparticles with better in vivo compatibility.

Key words: Magnetic nanoparticles;　CDMNP-PEG-CD;　SOMNP;　CDMNP;　Immune response, Skeletal muscle　

中图分类号:

R318.08

菅晓婷, 王涵, 黄静雯, 蓝海强, 廖钊宏, 柯渔, 廖华, 诸春敏. CDMNP-PEG-CD磁纳米颗粒诱导的骨骼肌反应性分析[J]. 中国临床解剖学杂志, 2022, 40(2): 173-180.

Jian Xiaoting, Wan Han, Huang Jingwen, Lan Haiqiang, Liao Zhaohong, Ke Yu, Liao Hua, Zhu Chunmin. Analysis of skeletal muscle reactivity induced by CDMNP-PEG-CD magnetic nanoparticles[J]. Chinese Journal of Clinical Anatomy, 2022, 40(2): 173-180.

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CDMNP-PEG-CD磁纳米颗粒诱导的骨骼肌反应性分析

Analysis of skeletal muscle reactivity induced by CDMNP-PEG-CD magnetic nanoparticles

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