长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究

doi:10.13418/j.issn.1001-165x.2022.2.13

中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (2): 187-191.doi: 10.13418/j.issn.1001-165x.2022.2.13

长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究

蔡昊¹，陈春²*

1.温州医科大学附属第一临床医学院，浙江温州 325000； 2.温州医科大学附属第一医院创伤关节科，浙江温州 325000

收稿日期:2021-08-10 出版日期:2022-03-25 发布日期:2022-04-12
通讯作者: 陈春，副主任医师，副教授，硕士生导师，E-mail：chenchunkk@163.com
作者简介:蔡昊（2000-），男，浙江温州人，在读本科，研究方向：创伤关节基础及临床，E-mail：514890909@qq.com
基金资助:
浙江省大学生科技创新活动计划（新苗人才计划）资助（2020R413006）；浙江省医药卫生科技计划（2021KY206）；温州市公益性科技计划（Y20190267）

Expression and correlation of long non-coding lncRNA-MEG3 in steroid-induced femoral head necrosis

Cai Hao¹, Chen Chun²*

1. The First Clinical Medical College, Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China; 2. Department of Traumatology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China

Received:2021-08-10 Online:2022-03-25 Published:2022-04-12

摘要/Abstract

摘要： 目的探讨长链非编码RNA-MEG3（lncRNA-MEG3）在激素性股骨头坏死中的表达及意义。方法应用实时荧光定量PCR方法检测股骨颈骨折患者股骨头组织（30例）和激素性股骨头坏死患者的股骨头及其旁组织（30对）中LncRNA-MEG3的表达并分析其临床意义。结果激素性坏死的股骨头骨组织中lncRNA-MEG3的含量最高，其次为其旁组织，股骨颈骨折患者骨组织中含量最低，差异均有统计学意义（P<0.05）；两组间患者的性别比例、年龄（<60岁或≥60岁）比例无显著性差异（P>0.05）；股骨头坏死组，服用不同种类激素（甲泼尼龙、地塞米松）的患者间lncRNA-MEG3含量无显著差别（P>0.05）；在不同ARCO分期（III期或IV期）患者中，IV期lncRNA-MEG3相对表达量明显高于III期（P<0.05）。结论长链非编码RNA-MEG3的表达与激素性股骨头坏死关系密切，且它的表达与激素性股骨头坏死的ARCO分期相关。

关键词: 长链非编码RNA； , , MEG3； , , 股骨头坏死； , , 激素

Abstract: Objective To investigate the expression and significance of long non-coding RNA-MEG3(lncRNA-MEG3) in steroid-induced femoral head necrosis. Methods Real-time fluorescence quantitative PCR was used to detect the expression of lncRNA-MEG3 in femoral head tissues of patients with femoral neck fracture (30 cases) and femoral head and its adjacent tissues of patients with steroid-induced femoral head necrosis (30 pairs), and its clinical significance was analyzed. Results The content of lncRNA-MEG3 was the highest in the femoral head bone tissues with steroid-induced necrosis, its adjacent tissues was followed, and the lowest was the bone tissues of patients with femoral neck fracture, the difference was statistically significant (P<0.05). There was no significant difference in the ratio of gender and age(less than 60 years old or more than 60 years old) between the two groups(P>0.05). In the femoral head necrosis group, there was no significant difference in lncRNA-MEG3 content among patients taking different hormone types (meprednisone or dexamethasone) (P>0.05). In patients with different ARCO stages (stage Ⅲ orⅣ), the relative expression of lncRNA-MEG3 in stage IV was higher that of stage Ⅲ (P<0.05). Conclusions The expression of long non-coding RNA-MEG3 is closely related to steroid-induced femoral head necrosis, and its expression is related to ARCO stage of steroid-induced femoral head necrosis.

Key words: Long non-coding RNA; , , MEG3; , , Femoral head necrosis; , , Hormone

中图分类号:

R681.8

蔡昊, 陈春. 长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究[J]. 中国临床解剖学杂志, 2022, 40(2): 187-191.

Cai Hao, Chen Chun. Expression and correlation of long non-coding lncRNA-MEG3 in steroid-induced femoral head necrosis[J]. Chinese Journal of Clinical Anatomy, 2022, 40(2): 187-191.

参考文献 18

[1]	梁大伟, 杨琼, 裴佳, 等. 478例股骨头坏死患者流行病学调查研究[J]. 临床骨科杂志, 2020, 23(5): 699-702. DOI: 10.3969/j.issn.1008-0287.2020.05.033.
[2]	Wu X, Sun W, Tan M. Noncoding RNAs in steroid-induced osteonecrosis of the femoral head[J]. Biomed Res Int, 2019, 2019: 8140595. DOI: 10.1155/2019/8140595.
[3]	Zhao DW, Zhang F, Wang BJ, et al. Guidelines for clinical diagnosis and treatment of osteonecrosis of the femoral head in adults (2019 version)[J]. J Orthop Translat, 2020, 21: 100-110. DOI: 10.1016/j.jot.2019.12.004.
[4]	段毅, 易谭勇, 朱乐全, 等. LncRNA MEG3调控miR-328影响类风湿性关节炎滑膜细胞增殖、凋亡和炎性反应[J]. 中国组织化学与细胞化学杂志, 2021, 30(2): 136-145. DOI: 10.16705/j.cnki.1004-1850.2021.02.004.
[5]	Chang C, Greenspan A, Gershwin ME. The pathogenesis, diagnosis and clinical manifestations of steroid-induced osteonecrosis[J]. J Autoimmun, 2020, 110: 102460. DOI: 10.1016/j.jaut.2020.102460.
[6]	Zhao XY, Lin JD. Long noncoding RNAs: a new regulatory code in metabolic control[J]. Trends Biochem Sci, 2015, 40(10): 586-596. DOI: 10.1016/j.tibs.2015.08.002.
[7]	Choudhry H, Harris AL, McIntyre A. The tumour hypoxia induced non-coding transcriptome[J]. Mol Aspects Med, 2016, (47-48): 35-53. DOI: 10.1016/j.mam.2016.01.003.
[8]	Briggs JA, Wolvetang EJ, Mattick JS, et al. Mechanisms of long non-coding RNAs in mammalian nervous system development, plasticity, disease, and evolution[J]. Neuron, 2015, 88(5): 861-877. DOI: 10.1016/j.neuron.2015.09.045.
[9]	Yan HL, Yuan JP, Gao LK, et al. Long noncoding RNA LncRNA-MEG3 activation of p53 mediates ischemic neuronal death in stroke[J]. Neuroscience, 2016, 337: 191-199. DOI: 10.1016/j.neuroscience. 2016. 09.017.
[10]	张轩铭, 吕松岑. 长链非编码RNA LncRNA-MEG3对成骨分化及骨性疾病影响的研究进展[J].实用骨科杂志, 2021, 27(2), 148-152. DOI: 10.13795/j.cnki.sgkz.2021.02.011.
[11]	赵轶男, 袁志, 毕龙, 等. LncRNA LncRNA-MEG3通过抑制Wnt/β-catenin信号通路对骨关节炎软骨细胞增殖和凋亡的影响[J]. 现代生物医学进展, 2019, 19(24): 27-33. DOI: 10.13241/j.cnki.pmb. 2019.24. 004.
[12]	Wang GQ, Ye QW, Ning SJ, et al. LncRNA LncRNA-MEG3 promotes endoplasmic reticulum stress and suppresses proliferation and invasion of colorectal carcinoma cells through the LncRNA-MEG3/miR-103a-3p/PDHB ceRNA pathway[J]. Neoplasma, 2021, 68(2): 362-374. DOI: 10.4149/neo_2020_200813N858.
[13]	Qiu GZ, Tian W, Fu HT, et al. Long noncoding RNA-LncRNA-MEG3 is involved in diabetes mellitus-related microvascular dysfunction[J]. Biochem Biophys Res Commun, 2016, 471(1): 135-141. DOI: 10.1016/j.bbrc.2016.01.164.
[14]	Wallace C, Smyth DJ, Maisuria-Armer M, et al. The imprinted DLK1-LncRNA-MEG3 gene region on chromosome 14q32.2 alters susceptibility to type 1 diabetes[J]. Nat Genet, 2010, 42(1): 68-71. DOI: 10.1038/ng.493.
[15]	Chen RP, Huang ZL, Liu LX, et al. Involvement of endoplasmic reticulum stress and p53 in lncRNA LncRNA-MEG3-induced human hepatoma HepG2 cell apoptosis[J]. Oncol Rep, 2016, 36(3): 1649-1657. DOI: 10.3892/or.2016.4919.
[16]	Xia Y, He ZC, Liu B, et al. Downregulation of LncRNA-MEG3 enhances cisplatin resistance of lung cancer cells through activation of the WNT/beta-catenin signaling pathway[J]. Mole Med Rep, 2015, 12(3): 4530-4537. DOI: 10.3892/mmr.2015.3897.
[17]	Teng GY, Wang YJ, Geng M, et al. LncRNA MEG3 inhibits the growth, invasion and migration of Wilms' tumor via Wnt/β-catenin pathway[J]. Eur Rev Med Pharmacol Sci, 2020, 24(19): 9899-9907. DOI: 10.26355/eurrev_202010_23200.
[18]	姚金池, 杨春露, 车敏, 等. LncRNA MEG3在骨肉瘤组织中的表达及对MG-63细胞增殖、凋亡与侵袭的影响[J]. 解剖科学进展, 2020, 26(2): 150-153. DOI: 10.16695/j.cnki.1006-2947.2020.02.008.

长链非编码LncRNA-MEG3在激素性股骨头坏死中的表达及相关性研究

Expression and correlation of long non-coding lncRNA-MEG3 in steroid-induced femoral head necrosis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 18

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	江剑宏, 段仁鹏, 李晓锋. 国人胆道三维重建解剖变异应用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 1-4.
[2]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[3]	李佳伟, 张静, 李灿然, 兰文杰, 籍庆余, 郭志勇, 张云凤, 刘启, 陈清威, 李筱贺. 蒙古族人群股骨近端解剖参数X线测量[J]. 中国临床解剖学杂志, 2024, 42(1): 11-16.
[4]	李琨, 张燕, 郭冉, 郝咪咪, 吴轩宇, 徐艺芳, 王超群, 马文童, 张灵淇, 杨宏宇, 李志军, 张少杰, 王星. 儿童及青少年枕颈角与后枕颈角数字化测量及其临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 17-20.
[5]	李文, 杨思艺, 黄蕾, 卿霁雯, 蒋松涛, 张磊. 基于MRI探讨Lisfranc韧带的形态学特点及临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 21-25.
[6]	李凡凡, 徐阳, 王晓旭. 紫草素调节Nrf2/HO-1信号通路对实验性大鼠肉芽肿性小叶性乳腺炎的治疗作用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 26-32.
[7]	王兴航, 丁佳媛, 李放, 包翠芬, 阎丽菁. 人参皂苷Rg1通过抑制NLRP3炎症小体途径减轻氧糖剥夺/复供后小胶质细胞炎症反应[J]. 中国临床解剖学杂志, 2024, 42(1): 33-41.
[8]	程莹莹, 武建军, 蔡海燕, 焦旭文, 马江波, 丁银秀. 体外炎性诱导星形胶质细胞激活及功能分析[J]. 中国临床解剖学杂志, 2024, 42(1): 42-46.
[9]	祁志, 杨力, 贾秋叶, 陈浩伦, 段兆达, 吴春云. 依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控[J]. 中国临床解剖学杂志, 2024, 42(1): 47-53.
[10]	张晶晶, 王洪新. 黄芩苷通过PDGF/P38 MAPK信号通路对肺动脉高压大鼠的保护作用[J]. 中国临床解剖学杂志, 2024, 42(1): 54-58.
[11]	乐钦, 陈荣丽, 熊婧熙, 王婷怡, 蔡志恒, 易秋实, 曾心怡. 白芨多糖对大鼠跨区皮瓣choke区的影响[J]. 中国临床解剖学杂志, 2024, 42(1): 59-64.
[12]	李笑予, 张磊, 付磊, 李东波, 汪国友. 三步接骨法治疗Sanders Ⅱ型跟骨骨折的有限元研究[J]. 中国临床解剖学杂志, 2024, 42(1): 65-70.
[13]	吴研飞, 马剑雄, 卢斌, 王颖, 柏豪豪, 靳洪震, 马信龙. CT后处理技术重建CT值与终板抗压强度的相关性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 71-76.
[14]	庄万强, 唐毅, 骆勇刚, 张辉. 关节镜联合胫骨高位截骨术对髌骨位置及髌股关节功能影响的早中期回顾性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 77-82.
[15]	罗鹰, 窦伟誉, 吴小杭, 陈璟昆, 彭昌贵, 潘剑英 . 微创海马钢板内固定治疗跟骨骨折并发症的影响因素[J]. 中国临床解剖学杂志, 2024, 42(1): 83-88.