pEGFR的表达与大鼠脊髓压迫性损伤后神经功能恢复的关系

doi:10.13418/j.issn.1001-165x. 2022.3.10

中国临床解剖学杂志 ›› 2022, Vol. 40 ›› Issue (3): 297-302.doi: 10.13418/j.issn.1001-165x. 2022.3.10

pEGFR的表达与大鼠脊髓压迫性损伤后神经功能恢复的关系

张敏¹，　李兴琼²，　晏四斤²，　李腊梅²，　杜瑶²，　曾庆盈²，　潘利锋²，　龚睿²

1.重庆医科大学附属永川医院内分泌科，重庆永川 402160； 2.重庆市永川区中医院推拿科，重庆永川 402160

收稿日期:2021-04-16 出版日期:2022-05-25 发布日期:2022-06-02
通讯作者: 龚睿，硕士研究生，E-mail：543754403@qq.com
作者简介:张敏（1990-），女，硕士研究生，内分泌科医师，主要研究方向：神经脱髓鞘病变的机制及治疗，E-mail：674182688@qq.com
基金资助:
重庆市研究生科研创新项目（40040200100410），重庆市永川区科委课题（Ycstc，2016nc5023）

Relationship between expression of pEGFR and neural function recovery after decompression of compressed spinal cord injury in rats

Zhang Min¹, Li Xingqiong², Yan Sijin², Li Lamei², Du Yao², Zeng Qingying², Pan Lifeng², Gong Rui²

1. Department of Endocrine, Yongchuan Hospital of Chongqing Medical University, Yongchuan 402160, Chongqing, China; 2.Department of Massage, Yongchuan Hospital of Traditional Chinese Medicine, Yongchuan 402160, Chongqing, China

Received:2021-04-16 Online:2022-05-25 Published:2022-06-02

摘要/Abstract

摘要： 目的　探讨大鼠活化型表皮生长因子受体（phosphorylated epidermal growth factor receptor，pEGFR）表达与脊髓压迫性损伤（compressed spinal cord injury，CSCI）后神经功能恢复的关系及可能的机制。方法　制备大鼠脊髓压迫性损伤模型，运用BBB（Basso Beattie Bresnahan）评分观察动物解压后神经功能的恢复情况，免疫印迹（Western blotting，WB）检测pEGFR、total-caspase-3、active-caspase-3的表达变化，免疫荧光双标（double-labeling immunoflurescence）检测pEGFR-NG2+、active-caspase-3-NG2+细胞的变化。结果　CSCI解压后，BBB评分随时间延长而逐渐增加；pEGFR的表达、pEGFR-NG2+细胞的数量随时间延长而逐渐增加并于解压后14 d达到高峰，而total-caspase-3、active-caspase-3的表达和active-caspase-3-NG2+细胞的数量在解压后即达到高峰并随时间延长逐渐降低。腹腔注射pEGFR抑制剂14 d后，pEGFR的表达、大鼠BBB评分较未注射pEGFR抑制剂组明显降低；total-caspase-3、active-caspase-3的表达和active-caspase-3-NG2+细胞显著增加。结论　大鼠pEGFR的表达与CSCI解压后神经功能的恢复有关，其机制可能在于pEGFR参与了Caspase-3信号通路的调节。

关键词: 脊髓压迫性损伤；　神经功能恢复；　pEGFR； , , Caspase-3

Abstract: Objective　To investigate the relationship between expression of phosphorylated epidermal growth factor receptor (pEGFR) and neural function recovery after decompression of compressed spinal cord injury(CSCI) in rats and clarify its possible mechanisms.　Methods　The CSCI model was established. The neural function recovery after decompression were monitored by the Basso, Beattie &Bresnahan(BBB) locomotor rating scale; the expression of pEGFR, total-caspase-3, active-caspase-3 were detected by Western blot; pEGFR+-NG2+(NG2+ cells are precursors to oligodendrocytes)，active-caspase-3+-NG2+ cells were detected by double-labeling immunefluorescence assay.　Results　The BBB scores gradually increased with time after decompression of CSCI. Meanwhile, the expression of pEGFR, number of pEGFR+-NG2+ cells were up-regulated and reached the peak at day 14, consistent with the changes of motor functions. total-caspase-3, active-caspase-3 and caspase-3-NG2+ cells immediately reached the peak after decompression and gradually decreased with time. After intraperitoneal injection of pEGFR inhibitor for 14 days, expression of pEGFR and BBB scores of rats were significantly lower than those of group without pEGFR inhibitor injection. The expression of total-caspase-3, active-caspase-3, caspase-3-NG2+ cells significantly increased.　Conclusions　The expression of pEGFR is related to process of neural function recovery after decompression of CSCI in rats, and its mechanism may be due to the involvement of pEGFR in the regulation of Caspase-3 signaling pathway.

Key words: Compressed Spinal Cord injury; , , Neural function recovery; , , pEGFR; , , Caspase-3

中图分类号:

张敏, 李兴琼, 晏四斤, 李腊梅, 杜瑶, 曾庆盈, 潘利锋, 龚睿. pEGFR的表达与大鼠脊髓压迫性损伤后神经功能恢复的关系[J]. 中国临床解剖学杂志, 2022, 40(3): 297-302.

Zhang Min, Li Xingqiong, Yan Sijin, Li Lamei, Du Yao, Zeng Qingying, Pan Lifeng, Gong Rui. Relationship between expression of pEGFR and neural function recovery after decompression of compressed spinal cord injury in rats[J]. Chinese Journal of Clinical Anatomy, 2022, 40(3): 297-302.

参考文献 10

[1]	Huang SQ, Tang CL, Sun SQ, et al. Demyelination initiated by oligodendrocyte apoptosis through enhancing endoplasmic reticulum-mitochondria interactions and Id2 expression after compressed spinal cord injury in rat[J]. CNS Neurosci Ther, 2014, 20(1): 20-31. DOI: 10.1111/cns.12155.
[2]	Furlan JC, Noonan V, Cadotte DW, et al. Timing of decompressive surgery of spinal cord after traumatic spinal cord injury: an evidence-based examination of pre-clinical and clinical studies[J]. J Neurotrauma, 2011, 28(8): 1371-1399. DOI: 10.1089/neu.2009.1147.
[3]	梁益建，孙善全，汪克建，等. 大鼠脊髓慢性压迫性损伤实验模型的建立[J].中国临床解剖学杂志, 2006, 24(3):320-324. DOI:10.13418/j.issn.1001-165x.2006.03.034.
[4]	Wnęk A, Andrzejewska E, Kobos J, et al. Molecular and immunohistochemical expression of apoptotic proteins Bax, Bcl-2 and Caspase 3 in infantile hemangioma tissues as an effect of propranolol treatment[J]. Immunol Lett, 2017, 185: 27-31.DOI: 10.1016/j.imlet. 2017.03.005.
[5]	Erschbamer M, Pernold K, Olson L. Inhibiting epidermal growth factor receptor improves structural, locomotor, sensory, and bladder recovery from experimental spinal cord injury[J]. J Neurosci, 2007, 27: 6428-6435. DOI: 10.1523/JNEUROSCI.1037-07.2007.
[6]	Li ZW, Li JJ, Wang L, et al. Epidermal growth factor receptor inhibitor ameliorates excessive astrogliosis and improves the regeneration microenvironment and functional recovery in adult rats following spinal cord injury[J]. J Neuroinflammation, 2014, 11: 71. DOI: 10.1186/1742-2094-11-71.
[7]	Sharp K, Yee KM, Steward O. A re-assessment of the effects of treatment with an epidermal growth factor receptor (EGFR) inhibitor on recovery of bladder and locomotor function following thoracic spinal cord injury in rats[J]. Exp Neurol, 2012, 233(2): 649-659. DOI: 10.1016/j.expneurol.2011.04.013.
[8]	Wen QQ, Zhang X, Cai JY, et al. A novel strategy for real-time and in situ detection of cytochrome c and caspase-9 in Hela cells during apoptosis[J]. Analyst, 2014, 139(10): 2499-2506. DOI: 10.1039/c3an02205f.
[9]	Wu Y, Kimura Y, Okamoto T, et al, et al. Inflammatory bowel disease-associated ubiquitin ligase RNF183 promotes lysosomal degradation of DR5 and TRAIL-induced caspase activation[J]. Sci Rep, 2019, 9(1): 20301. DOI: 10.1038/s41598-019-56748-6.
[10]	de Miguel D, Lemke J, Anel A, et al. Onto better TRAILs for cancer treatment[J]. Cell Death Differ, 2016, 23(12): 733-747. DOI: 10.1038/cdd.2015.174.

[1]	江剑宏, 段仁鹏, 李晓锋. 国人胆道三维重建解剖变异应用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 1-4.
[2]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[3]	李佳伟, 张静, 李灿然, 兰文杰, 籍庆余, 郭志勇, 张云凤, 刘启, 陈清威, 李筱贺. 蒙古族人群股骨近端解剖参数X线测量[J]. 中国临床解剖学杂志, 2024, 42(1): 11-16.
[4]	李琨, 张燕, 郭冉, 郝咪咪, 吴轩宇, 徐艺芳, 王超群, 马文童, 张灵淇, 杨宏宇, 李志军, 张少杰, 王星. 儿童及青少年枕颈角与后枕颈角数字化测量及其临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 17-20.
[5]	李文, 杨思艺, 黄蕾, 卿霁雯, 蒋松涛, 张磊. 基于MRI探讨Lisfranc韧带的形态学特点及临床意义[J]. 中国临床解剖学杂志, 2024, 42(1): 21-25.
[6]	李凡凡, 徐阳, 王晓旭. 紫草素调节Nrf2/HO-1信号通路对实验性大鼠肉芽肿性小叶性乳腺炎的治疗作用研究[J]. 中国临床解剖学杂志, 2024, 42(1): 26-32.
[7]	王兴航, 丁佳媛, 李放, 包翠芬, 阎丽菁. 人参皂苷Rg1通过抑制NLRP3炎症小体途径减轻氧糖剥夺/复供后小胶质细胞炎症反应[J]. 中国临床解剖学杂志, 2024, 42(1): 33-41.
[8]	程莹莹, 武建军, 蔡海燕, 焦旭文, 马江波, 丁银秀. 体外炎性诱导星形胶质细胞激活及功能分析[J]. 中国临床解剖学杂志, 2024, 42(1): 42-46.
[9]	祁志, 杨力, 贾秋叶, 陈浩伦, 段兆达, 吴春云. 依达拉奉经MAPKs通路对脂多糖激活的小胶质细胞Sirt3表达调控[J]. 中国临床解剖学杂志, 2024, 42(1): 47-53.
[10]	张晶晶, 王洪新. 黄芩苷通过PDGF/P38 MAPK信号通路对肺动脉高压大鼠的保护作用[J]. 中国临床解剖学杂志, 2024, 42(1): 54-58.
[11]	乐钦, 陈荣丽, 熊婧熙, 王婷怡, 蔡志恒, 易秋实, 曾心怡. 白芨多糖对大鼠跨区皮瓣choke区的影响[J]. 中国临床解剖学杂志, 2024, 42(1): 59-64.
[12]	李笑予, 张磊, 付磊, 李东波, 汪国友. 三步接骨法治疗Sanders Ⅱ型跟骨骨折的有限元研究[J]. 中国临床解剖学杂志, 2024, 42(1): 65-70.
[13]	吴研飞, 马剑雄, 卢斌, 王颖, 柏豪豪, 靳洪震, 马信龙. CT后处理技术重建CT值与终板抗压强度的相关性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 71-76.
[14]	庄万强, 唐毅, 骆勇刚, 张辉. 关节镜联合胫骨高位截骨术对髌骨位置及髌股关节功能影响的早中期回顾性研究[J]. 中国临床解剖学杂志, 2024, 42(1): 77-82.
[15]	罗鹰, 窦伟誉, 吴小杭, 陈璟昆, 彭昌贵, 潘剑英 . 微创海马钢板内固定治疗跟骨骨折并发症的影响因素[J]. 中国临床解剖学杂志, 2024, 42(1): 83-88.

pEGFR的表达与大鼠脊髓压迫性损伤后神经功能恢复的关系

Relationship between expression of pEGFR and neural function recovery after decompression of compressed spinal cord injury in rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 10

相关文章 15

Metrics

本文评价

推荐阅读 0