miR-181a调控sirt1通路抑制七氟烷诱导的海马神经元凋亡

doi:10.13418/j.issn.1001-165x.2023.2.14

中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (2): 200-206.doi: 10.13418/j.issn.1001-165x.2023.2.14

miR-181a调控sirt1通路抑制七氟烷诱导的海马神经元凋亡

李丽楠，丁汉琳，刘金川*

湖北文理学院附属医院，襄阳市中心医院疼痛科，湖北襄阳 441021

收稿日期:2021-12-28 出版日期:2023-03-25 发布日期:2023-04-14
通讯作者: 刘金川，硕士，副主任医师，E-mail：651227340@qq.com
作者简介:李丽楠（1985-），女，硕士，副主任医师，研究方向：镇痛麻醉，E-mail：lhwogi@163.com
基金资助:
湖北省自然科学基金（WJ2017Q039）

Effect of miR-181a on the apoptosis of hippocampal neurons induced by sevoflurane by regulating the sirt1 pathway

Li Linan, Ding Hanlin, Liu Jinchuan*

Department of Pain, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Sciences， Xiangyang 441021, Hubei Province, China

Received:2021-12-28 Online:2023-03-25 Published:2023-04-14

摘要/Abstract

摘要： 目的探讨miR-181a对七氟烷诱导海马神经元凋亡的影响及其机制。方法验证miR-181a与沉默信息调节因子1（silent information regulator1，sirt1）的关系，检测七氟烷对海马神经元中miR-181a表达及细胞凋亡的影响；将海马神经元转染后用3%七氟烷处理4 h，分为空白组、miR-NC组、miR-181a组、anti-miR-NC组、anti-miR-181a组、（anti-miR-NC+si-NC）组、（anti-miR-181a+si-NC）组、（anti-miR-181a+si-sirt1）组，检测各组细胞中sirt1、核因子κB（NF-κB）、sirt1蛋白、miR-181a表达及细胞凋亡情况；体内实验检测七氟烷处理或同时抑制miR-181a和sirt1表达后海马组织中神经元凋亡变化。结果 miR-181a靶向调控sirt1表达；七氟烷使海马神经元中miR-181a表达及细胞凋亡率升高（P<0.05）；上调miR-181a抑制sirt1蛋白表达，促进NF-κB蛋白表达，而抑制miR-181a呈相反趋势（P<0.05）；体内与体外实验均证实沉默sirt1可减弱下调miR-181a对七氟烷诱导的海马神经元凋亡的抑制作用。结论沉默miR-181a通过激活七氟烷诱导的海马神经元中sirt1/NF-κB通路，抑制海马神经元凋亡。

关键词: 七氟烷, , , 海马神经元, , , 沉默信息调节因子1, , , 凋亡

Abstract: Objective To explore the effect of miR-181a on the apoptosis of hippocampal neurons induced by sevoflurane and its mechanism. Methods The relationship between miR-181a and silent information regulator 1 (sirt1) was verified. The effect of sevoflurane on miR-181a expression and apoptosis in hippocampal neurons was detected. Hippocampal neurons were transfected with 3% sevoflurane Alkane treatment for 4 h. They were divided into the following group: a blank group, a miR-NC group, a miR-181a group, an anti-miR-NC group, an anti-miR-181a group, an anti-miR-NC+si-NC group, an anti-miR-181a +si-NC group and an anti-miR-181a+si-sirt1 group. The expression of sirt1, nuclear factor kappa B (NF-κB), sirt1 protein, miR-181a and apoptosis of cells in each group were detected. The changes of neuronal apoptosis in hippocampus were detected after sevoflurane treatment or simultaneous inhibition of miR-181a and sirt1 expression by in vivo experiments. Results miR-181a regulated the expression of sirt1 by targeting. After sevoflurane treatment, the expression of miR-181a and the apoptosis rate in hippocampal neurons increased (P<0.05). Up-regulation of miR-181a inhibited the expression of sirt1 protein and promoted the expression of NF-κB protein, while inhibiting miR-181a showed the opposite trend (P<0.05). In vivo and in vitro experiments confirmed that silencing sirt1 attenuated the inhibitory effect of down-regulated miR-181a on sevoflurane-induced apoptosis in hippocampal neurons. Conclusions Silencing miR-181a inhibits hippocampal neuronal apoptosis by activating the sirt1/NF-κB pathway in hippocampal neurons treated with sevoflurane.

Key words: Sevoflurane, , , Hippocampal neurons, , , Silent information regulator 1, , , , Apoptosis

中图分类号:

R742.1

李丽楠, 丁汉琳, 刘金川. miR-181a调控sirt1通路抑制七氟烷诱导的海马神经元凋亡[J]. 中国临床解剖学杂志, 2023, 41(2): 200-206.

Li Linan, Ding Hanlin, Liu Jinchuan. Effect of miR-181a on the apoptosis of hippocampal neurons induced by sevoflurane by regulating the sirt1 pathway[J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 200-206.

参考文献 11

[1]	肖秀英, 吴华兵, 詹玮玮. 丙泊酚对七氟烷引起的大鼠海马神经细胞凋亡及认知障碍的影响及相关机制探究[J]. 解放军医药杂志, 2021, 33(2): 10-13. DOI: 10.3969/j.issn.2095-140X.2021.02.003.
[2]	刘颖, 夏中元, 刘康, 等.内质网应激/蛋白激酶样内质网激酶/DNA损伤诱导因子153通路在七氟烷致大鼠海马神经元细胞凋亡中的作用[J]. 中华实验外科杂志, 2019(9): 1618-1621. DOI: 10.3760/cma.j.issn.1001-9030.2019.09.026.
[3]	Liu X, Hong L, Peng W, et al. The neuroprotective effect of miR-181a after oxygen-glucose deprivation/reperfusion and the associated mechanism[J]. J Mol Neurosci, 2019, 68(2): 261-274. DOI: 10.1007/s12031-019-01300-4.
[4]	Zhu Y, Wang K, Ma Z, et al. SIRT1 activation by butein attenuates sepsis-induced brain injury in mice subjected to cecal ligation and puncture via alleviating inflammatory and oxidative stress[J]. Toxicol Appl Pharmacol,2019, 363(1): 34-46. DOI: 10.1016/j.taap. 2018. 10.013.
[5]	Song H, Xun S, He H, et al. Compound porcine cerebroside and ganglioside injection (CPCGI) attenuates sevoflurane-induced nerve cell injury by regulating the phosphorylation of p38 MAP kinase (p38MAPK)/nuclear factor kappa B (NF-κB) pathway[J]. Med Sci Monit, 2020, 26(1): 1-8. DOI: 10.12659/MSM.919600.
[6]	赵晨璐, 赵以林, 张雪. miR-34a通过Wnt/β-catenin通路在氯胺酮致发育期大鼠海马神经元凋亡中的作用[J]. 郑州大学学报(医学版), 2021, 56(1): 6-11. DOI: 10.13705/j.issn. 1671-6825.2020.01.002.
[7]	马大亮, 崔红莉, 荣卫江, 等. 柴胡皂苷A减轻脑缺血再灌注大鼠海马神经元损伤[J]. 中国临床解剖学杂志, 2021, 39(5): 569-574. DOI: 10.13418/j.issn.1001-165x.2021.05.013.
[8]	刘伟, 潘吴君, 赵静雅, 等. MiR-181a调控海马神经元突触生长在癫痫发生中的作用及机制[J]. 中国老年学杂志, 2018, 38(5): 1176-1179. DOI: 10.3969/j.issn.1005-9202.2018.05.059.
[9]	Rodriguez-Ortiz CJ, Prieto GA, Martini AC, et al. MiR-181a negatively modulates synaptic plasticity in hippocampal cultures and its inhibition rescues memory deficits in a mouse model of Alzheimer's disease[J]. Aging Cell, 2020, 19(3): e13118. DOI: 10.1111/acel.13118.
[10]	易亚乔, 刘检, 刘林, 等. 加味脑泰方对大鼠缺氧/复氧损伤海马神经元炎性通路SIRT1/NF-κB的影响[J]. 中国中医药信息杂志, 2019, 26(3): 45-50. DOI: 10.3969/j.issn.1005-5304.2019.03.011.
[11]	Yan L, Zhu T. Effects of rosuvastatin on neuronal apoptosis in cerebral ischemic stroke rats via Sirt1/NF-kappa B signaling pathway[J]. Eur Rev Med Pharmacol Sci, 2019, 23(12): 5449-5455. DOI: 10.26355/eurrev_201906_18214.

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miR-181a调控sirt1通路抑制七氟烷诱导的海马神经元凋亡

Effect of miR-181a on the apoptosis of hippocampal neurons induced by sevoflurane by regulating the sirt1 pathway

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