神经肌电刺激通过调控炎症因子促进小鼠脊髓损伤修复研究

doi:10.13418/j.issn.1001-165x.2023.4.08

中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (4): 416-421.doi: 10.13418/j.issn.1001-165x.2023.4.08

神经肌电刺激通过调控炎症因子促进小鼠脊髓损伤修复研究

梁银花，宋海旺，姜冠华，罗艺，刘国祥，杨丹*

贵州医科大学基础医学院人体解剖学教研室，贵阳 550025

收稿日期:2022-11-18 出版日期:2023-07-25 发布日期:2023-08-02
通讯作者: 杨丹，博士，副教授，E-mail：stefanie111@126.com
作者简介:梁银花（1999-），女，贵州福泉人，本科，E-mail：485368739@qq.com
基金资助:
贵州省教育厅青年科技人才成长项目（黔教合KY字[2022]226号）；贵州省卫生健康委科学技术基金项目（gzwkj2021-262）；贵州医科大学国家自然科学基金培育项目（19NSP032）；大学生创新训练项目（202110660020）

Neuromuscular electrical stimulation accelerates spinal cord injury repair by regulating inflammatory factors

Liang Yinhua, Song Haiwang, Jiang Guanhua, Lou Yi, Liu Guoxiang, Yang Dan*

Department of Human Anatomy, School of Basic Medicine, Guizhou Medical University, Guiyang 550025, China

Received:2022-11-18 Online:2023-07-25 Published:2023-08-02

摘要/Abstract

摘要： 目的探讨神经肌电刺激（neuromuscular electrical stimulation，NMES）调控炎症因子表达修复脊髓损伤（spinal cord injury，SCI）的机制。方法将60只健康成年小鼠随机分为假手术组（Sham组）、SCI组、1 Hz NMES组、20 Hz NMES组，每组15只，利用Basso Mouse Scale评分和足印分析观察小鼠后肢运动功能恢复情况；免疫荧光观察脊髓损伤前角神经元数目；real-time RT-PCR检测炎症因子TNF-α、IL-6、IL-12 mRNA表达；Western blot测定炎症因子TNF-α、IL-6、IL-12及BDNF、GFAP表达。结果与SCI组相比，神经肌电刺激干预后小鼠后肢运动功能明显改善，损伤处存活的神经元增多，且20 Hz NMES组神经元多于1 Hz NMES组；神经肌电刺激干预可以下调TNF-α、IL-6、IL-12及GFAP表达，促进BDNF表达。结论神经肌电刺激能下调炎症因子抑制GFAP表达，抑制纤维瘢痕形成，同时上调BDNF表达保护神经元数目，促进脊髓损伤后小鼠运动功能恢复。

关键词: 脊髓损伤, , , 神经肌电刺激, , , 炎症因子, , , 功能恢复

Abstract: Objective To investigate the mechanism of neuromuscular electrical stimulation (NMES) regulating the expression of inflammatory factors in repairing spinal cord injury (SCI). Methods Sixty healthy adult mice were randomly divided into a Sham group, a SCI group, a 1 Hz NMES group, a 20 Hz NMES group, 15 mice in each group. Basso Mouse Scale (BMS) score and footprint analysis were used to observe the recovery of hindlimb motor function of mice. Immunofluorescence was used to observe the number of anterior horn neurons of spinal cord injury. Real-time RT-PCR was used to detect the expression of inflammatory factors TNF-α, IL-6 and IL-12 mRNA. Western Blot was used to determine the inflammatory factors TNF-α, IL-6, IL-12 and the expression of BDNF, GFAP. Results Compared with the SCI group, the hindlimb motor function of mice was significantly improved after the neuromuscular electrical stimulation intervention, and the number of surviving neurons at the injury site was increased, and the number of neurons in the 20 Hz NMES group was more than that in the 1 Hz NMES group. Neuromuscular electrical stimulation intervention could down-regulate the expression of TNF-α, IL-6, IL-12 and GFAP, and promote the expression of BDNF. Conclusions Neuromuscular electrical stimulation can down-regulate inflammatory factors, inhibit the expression of GFAP, inhibit glial scarring, and up-regulate the expression of BDNF to protect the number of neurons, and promote the recovery of motor function in mice after spinal cord injury.

Key words: Spinal cord injury, , , , Neuromuscular electrical stimulation, , , Inflammatory factor, Functional recovery

中图分类号:

梁银花, 宋海旺, 姜冠华, 罗艺, 刘国祥, 杨丹. 神经肌电刺激通过调控炎症因子促进小鼠脊髓损伤修复研究[J]. 中国临床解剖学杂志, 2023, 41(4): 416-421.

Liang Yinhua, Song Haiwang, Jiang Guanhua, Lou Yi, Liu Guoxiang, Yang Dan . Neuromuscular electrical stimulation accelerates spinal cord injury repair by regulating inflammatory factors [J]. Chinese Journal of Clinical Anatomy, 2023, 41(4): 416-421.

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神经肌电刺激通过调控炎症因子促进小鼠脊髓损伤修复研究

Neuromuscular electrical stimulation accelerates spinal cord injury repair by regulating inflammatory factors

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