LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究

doi:10.13418/j.issn.1001-165x.2023.5.08

中国临床解剖学杂志 ›› 2023, Vol. 41 ›› Issue (5): 543-549.doi: 10.13418/j.issn.1001-165x.2023.5.08

LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究

郑锴¹，罗秀玲²，张玮豪¹，刘宇利¹，李玉明¹，廖尚高³

贵州医科大学 1.附属医院神经外科， 2.附属医院头颈肿瘤科， 3.药学院，贵阳 550004

收稿日期:2022-04-22 出版日期:2023-09-25 发布日期:2023-10-16
作者简介:郑锴（1972-），男，医学博士，副主任医师，研究方向：神经创伤与肿瘤，E-mail：ZK1972Z@163.com
基金资助:
贵州省高层次创新型人才资助项目（黔科合平台人才[2020]6011）

Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells

Zheng Kai ¹, Luo Xiuling², Zhang Weihao¹, Liu Yuli ¹, Li Yuming ¹, Liao Shanggao³

1. Department of Neurosurgery, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2. Department of Head and Neck Oncology, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou province, China; 3. School of Pharmacy, Guizhou Medical University, Guiyang 550004, Guizhou province, China

Received:2022-04-22 Online:2023-09-25 Published:2023-10-16

摘要/Abstract

摘要： 目的探讨lncRNA NEAT1对垂体瘤细胞侵袭、迁移的作用及机制。方法 qRT-PCR检测lncRNA NEAT1、miR-134和ITGB1表达；Transwell检测侵袭和迁移；双荧光素酶报告基因实验检测miR-134与lncRNA NEAT1、ITGB1的调控关系；Western blot检测ITGB1/FAK/PI3K通路相关蛋白表达。结果敲低lncRNA NEAT1抑制GH3细胞侵袭和迁移（P<0.05）；抑制miR-134可逆转下调lncRNA NEAT1对GH3细胞侵袭、迁移的抑制作用（P<0.05）。miR-134与lncRNA NEAT1和ITGB1存在负靶向调控关系（P<0.05）。上调miR-134抑制GH3细胞侵袭和迁移，抑制ITGB1/FAK/PI3K通路激活（P<0.05）；ITGB1过表达削弱上调miR-134对GH3细胞的影响（P<0.05）。结论下调lncRNA NEAT1靶向miR-134可能通过抑制ITGB1/FAK/PI3K通路激活抑制垂体瘤细胞侵袭、迁移。

关键词: lncRNA NEAT1； , , miR-134； , , 垂体瘤； , , 侵袭； , , 迁移

Abstract: Objective To explore the effect and potential mechanism of lncRNA NEAT1 on the invasion and migration of pituitary tumor cells. Methods The expressions of lncRNA NEAT1, miR-134, and ITGB1 were detected by qRT-PCR. Transwell was used to detect the invasion and migration. The regulatory relationship between miR-134 and lncRNA NEAT1 and ITGB1 was detected by a double luciferase reporter gene experiment. The expression of ITGB1/FAK/PI3K pathway-related proteins was detected by Western blot. Results Knockdown of lncRNA NEAT1 inhibited the invasion and migration of GH3 cells (P<0.05); inhibition of miR-134 could reverse the inhibitory effect of down-regulated lncRNA NEAT1 on the invasion and migration of GH3 cells (P<0.05). miR-134 had a negative targeted regulation relationship with lncRNAs NEAT1 and ITGB1 (P<0.05). Up-regulation of miR-134 inhibited the invasion and migration of GH3 cells and inhibited the activation of the ITGB1/FAK/PI3K pathway (P<0.05). ITGB1 overexpression attenuated the effect of up-regulated miR-134 on GH3 cells (P<0.05). Conclusions Down-regulation of lncRNA NEAT1 targeting miR-134 may inhibit the invasion and migration of pituitary tumor cells by inhibiting the activation of the ITGB1/FAK/PI3K pathway.

Key words: lncRNA NEAT1, 　miR-134, 　Pituitary tumor, 　Invasion, 　Migration

中图分类号:

R736.4

郑锴, 罗秀玲, 张玮豪, 刘宇利, 李玉明, 廖尚高. LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究[J]. 中国临床解剖学杂志, 2023, 41(5): 543-549.

Zheng Kai, Luo Xiuling, Zhang Weihao, Liu Yuli, Li Yuming, Liao Shanggao. Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells[J]. Chinese Journal of Clinical Anatomy, 2023, 41(5): 543-549.

参考文献 14

[1]	Wang L, Cho KB, Li Y, et al. Long Noncoding RNA (lncRNA)-mediated competing endogenous RNA networks provide novel potential biomarkers and therapeutic targets for colorectal cancer[J]. Int J Mol Sci, 2019, 20(22): 5758-5764. DOI: 10.3390/ijms20225758.
[2]	Li X, Deng S, Pang X, et al. lncRNA NEAT1 silenced miR-133b promotes migration and invasion of breast cancer cells[J]. Int J Mol Sci, 2019, 20(15): 3616-3621. DOI: 10.3390/ijms20153616.
[3]	庞其军, 赵颖, 郗艳国, 等. miR-134在人垂体腺瘤组织中的表达及其意义[J]. 现代生物医学进展, 2014, 14(17): 3328-3333. DOI: 10.13241/j.cnki.pmb.2014.17.034.
[4]	马丽媛, 代从新. 抗血管内皮生长因子靶向治疗难治性垂体腺瘤和垂体腺癌研究进展[J]. 中国神经精神疾病杂志, 2020, 46(10): 625-628. DOI: 10.3969/j.issn.1002-0152.2020.10.011.
[5]	Beylerli O, Khasanov D, Gareev I, et al. Differential non-coding RNAs expression profiles of invasive and non-invasive pituitary adenomas[J]. Noncoding RNA Res, 2021, 6(3): 115-122. DOI: 10.1016/j.ncrna.2021.06.004.
[6]	Wang X, Li X, Wang Z. lncRNA MEG3 inhibits pituitary tumor development by participating in cell proliferation, apoptosis and EMT processes[J]. Oncol Rep,2021,45(4): 40-53.DOI: 10.3892/or.2021. 7991.
[7]	倪文. lncRNA MIR4435-2HG靶向miR-1-3p调控信号通路PI3K/Akt对胰腺癌细胞增殖,侵袭和迁移的机制研究[J]. 安徽医药, 2021, 25(3): 467-473. DOI: 10.3969/j.issn.1009-6469.2021.03.009.
[8]	Kou JT, Ma J, Zhu JQ, et al. lncRNA NEAT1 regulates proliferation, apoptosis and invasion of liver cancer[J]. Eur Rev Med Pharmacol Sci, 2020, 24(8): 4152-4160. DOI: 10.26355/eurrev_202004_20995.
[9]	周建华, 胡涛, 陈宇, 等. lncRNA NEAT1在人脑胶质瘤的表达变化及其与病人预后的关系[J]. 中国临床神经外科杂志, 2019, 24(3): 148-150. DOI: 10.13798/j.issn.1009-153X.2019.03.007.
[10]	Wang W, Ge L, Xu XJ, et al. lncRNA NEAT1 promotes endometrial cancer cell proliferation, migration and invasion by regulating the miR-144-3p/EZH2 axis[J]. Radiol Oncol, 2019, 53(4): 434-442. DOI: 10.2478/raon-2019-0051.
[11]	Chen CL, Zhang L, Jiao YR, et al. miR-134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP1 and MMP3 in vitro and in vivo[J]. FEBS Lett, 2019, 593(10): 1089-1101. DOI: 10.1002/1873-3468.13387.
[12]	Ellert-miklaszewska A, Poleszak K, Pasierbinska M, et al. Integrin signaling in glioma pathogenesis: from biology to therapy[J]. Int J Mol Sci, 2020, 21(3): 888-893. DOI: 10.3390/ijms21030888.
[13]	Wang N, Chang LL. Maspin suppresses cell invasion and migration in gastric cancer through inhibiting EMT and angiogenesis via ITGB1/FAK pathway[J]. Hum Cell, 2020, 33(3): 663-675. DOI: 10.1007/s13577-020-00345-7.
[14]	Wu YJ, Lin SH, Din ZH, et al. Sinulariolide inhibits gastric cancer cell migration and invasion through downregulation of the EMT process and suppression of FAK/PI3K/AKT/mTOR and MAPKs signaling pathways[J]. Mar Drugs, 2019, 17(12): 668-874. DOI: 10.3390/md17120668.

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LncRNA NEAT1对垂体瘤细胞系GH3侵袭和迁移的作用机制研究

Mechanism of LncRNA NEAT1 on invasion and migration of pituitary tumor cells

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