关键词: 骨盆骨折, 成骨细胞, 骨钙蛋白, 骨唾液酸蛋白, 压应力

Abstract:

Objective　To explore the effects of acetabular tridimensional memory fixation system（ATMFS） on the bone healing and the expression of osteocalcin and bone sialoprotein of canine iliopectineal crest fracture models.　Methods　15 adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum，which were fixed with ATMFS and steel plate respectively. The animals were examined at 2，4，6，8 and 12 weeks after operation by X-ray and macropathological observation. The expression of osteocalcin and bone sialoprotein were investigated by in situ hybridization, histological image analysis and real-time PCR respectively.　Results　There was no disordered bony callus appeared in ATMFS fixed bone, with the shorter healing time of it compared to that of steel plate fixation. The more strong expression of osteocalcin and bone sialoprotein can be found in fixation groups compared to that of the control, furthermore, significantly strong expression of them appeared in ATMFS group than that of steel plate group（P<0.05）, especially at the 6 weeks（P<0.01）after the operation.　Conclusions　ATMFS is valuable for the production of osteocalcin and bone sialoprotein during the process of bone healing by generating persistent compressive stress.

Key words: Pelvic fracture, Osteoblast, Osteocalcin, Bone sialoprotein, Compressive stress