基质细胞衍生因子-1α在皮质发育畸形大鼠海马中的表达

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (1): 72-76.

基质细胞衍生因子-1α在皮质发育畸形大鼠海马中的表达

张　毅，　晏　勇，　张建刚，　黄　敏，　贺　兴

重庆医科大学附属第一医院神经内科，重庆市神经病学重点实验室，重庆 400016

收稿日期:2010-08-09 发布日期:2011-01-24
通讯作者: 晏勇，教授,博士生导师，E-mail：yyanpro@yahoo.com.cn E-mail:zhang120yi@126.com
作者简介:张　毅（1983-），男，在读硕士，主要研究方向：难治性癫痫

The expression of SDF-1α in the malformation of cortical development model rats

ZHANG Yi, YAN Yong, ZHANG Jian-gang, HUANG Min, HE Xing

Neurology Department of the first Affiliated Hospital，Chongqing Medical University，Chongqing Key Laboratory of Neurology，Chongqing 400016，China

Received:2010-08-09 Published:2011-01-24

摘要/Abstract

摘要：

目的　了解基质细胞衍生因子-1α（SDF-1α）在皮质发育畸形（MCD）大鼠海马中的表达，探讨其在皮质发育畸形异常局部神经回路中的作用。方法　通过腹部X射线照射孕16dSD大鼠构建子代MCD模型，对30、60d对照组与实验组子鼠（P30、 P60）行HE、Timm染色以检测其组织学改变，免疫组化、RT-PCR及Western-Blot检测SDF-1α的表达情况。结果　①Timm染色示模型鼠海马出现苔藓纤维出芽；②免疫组化示SDF-1α在模型组海马CA1区异位神经元有表达；③RT-PCR和Western-Blot结果显示模型组P30、P60较同期的对照组表达降低。结论　SDF-1α的下调可能参与皮质发育畸形海马中异常神经回路的形成。

关键词: 皮质发育畸形, 基质细胞衍生因子-1&alpha, 苔藓纤维出芽, 癫痫

Abstract:

Objective　To get a message of the expression about stromal cell-derived factor-1α（SDF-1α）in the Malformations of cortical development（MCD）in rats model and investigate the effects in forming abnormal neural circuit in the MCD.　Methods　Sprague-Dawley pregnant rats exposed to X-irradiation on embryonic 16（E16）days can induce MCD in the offspring. We observed the histological changes of the brain with HE staining and Timm staining，and the expression of SDF-1α with immunohistochemistry，RT-PCR and Western-Blot in the pups at postnatal 30 days（P30）and postnatal 60 days（P60）.　Results　①Timm staining demonstrated mossy fiber sprouting was formed in the MCD model；②Immunohistochemical results showed that expression of SDF-1α appeared in the CA1 ectopic neurons of hippocampus；③From the results of RT-PCR and Western-Blot，we got a reduced expression of SDF-1α at P30 and P60 in the model group contrast to control group at the same period. Conclusion the downregulation of SDF-1α may play a role in forming abnormal neural circuit in hippocampus of MCD.

Key words: Malformations of cortical development, Stromal cell-derived factor-1α, , Mossy fiber sprouting, Epilepsy

中图分类号:

R742.1

张　毅, 晏　勇, 张建刚, 黄　敏, 贺　兴. 基质细胞衍生因子-1α在皮质发育畸形大鼠海马中的表达[J]. 中国临床解剖学杂志, 2011, 29(1): 72-76.

ZHANG 　Yi, YAN 　Yong, ZHANG Jian-Gang, HUANG 　Min, HE 　Xin. The expression of SDF-1α in the malformation of cortical development model rats[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(1): 72-76.

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