血管铸型技术检测组织工程多孔支架血管化的实验研究

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (1): 87-88.

血管铸型技术检测组织工程多孔支架血管化的实验研究

武延格，　杨　林，　孙学峰，　王　正

暨南大学第二临床医学院深圳市人民医院胸外科，广东深圳　518020

收稿日期:2010-08-25 发布日期:2011-01-24
通讯作者: 杨林，主任医师，硕士生导师,E-mail:yanglin70@ yahoo.　com E-mail:wuyange@126.com
作者简介:武延格（1981）,女,河北清河人,硕士,医师,主要从事组织工程气管方面的研究
基金资助:
国家自然科学基金资助（30500499）

Experimental study on revascularization detection in tissue engineered scaffold using vascular cast technique

WU Yan-ge, YANG Lin , SUN Xue-feng, WANG Zheng

Department of Thoracic Surgery, Shenzhen People's Hospital, the Second Clinical Medical College of Jinan University, Shenzhen 518020, China

Received:2010-08-25 Published:2011-01-24

摘要/Abstract

摘要：

目的　探索利用血管铸型技术观察组织工程多孔支架体内再血管化情况的可行性。　方法　将DegraPol多孔管状支架置入SD大鼠腹腔大网膜包埋1周，应用解剖学中的血管铸型技术，观察组织工程支架体内形成新生血管的情况。　结果　大网膜包埋后在DegraPol支架外部发现有较多的蓝色显影微细血管环绕，在微孔支架内部，蓝色显影穿透支架并沿支架内孔分布。　结论　血管铸型适合用于微血管检测，可作为组织工程人工器官再血管化检测的一种新方法。

关键词: 血管铸型, 组织工程, 支架, 再血管化

Abstract:

Objective　To explore the feasibility of vascular cast for revascularization of tissue engineered organ.　Method　Tissue engineered scaffolds DegraPol were implanted in rat omental 1 weeks. The anatomic technology of vascular cast was used to observe revascularization of DegraPol scaffold.　Results　 Vascular cast showed more blue vessels around the DegraPol scaffold in omental implants. Micro-vessels were found grew into the porous scaffold.　Conclusions　Vascular cast technique is suitable for detecing the microvascular, and could be used as a new method to observe revascularization of tissue engineered organ.

Key words: Vascular cast, Tissue-engineering, Scaffold, Revascularization

中图分类号:

Q819

武延格, 杨　林, 孙学峰, 王　正. 血管铸型技术检测组织工程多孔支架血管化的实验研究[J]. 中国临床解剖学杂志, 2011, 29(1): 87-88.

WU Yan-Ge, YANG 　Lin, SUN Xua-Feng, WANG 　Zheng. Experimental study on revascularization detection in tissue engineered scaffold using vascular cast technique[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(1): 87-88.

参考文献

[1] Griffith LG, Naughton G. Tissue engineering-current challenges and expanding opportunities
[J]. Science, 2002, 295(8): 1009-1014.

[2] 张　晔, 曾秉芳. 组织工程与血管形成
[J]. 国外医学骨科分册, 2003, 24(6): 363-367.

[3] 曾宪利, 裴国献. 组织工程骨血管化机理及策略的研究进展. 实用手外科杂志,2005, 19(3): 193-195.

[4] Bouhadir KH, Mooney DJ. Promoting angiogenesis in engineered tissues
[J]. J Drug Target, 2001, 9(6): 397-406.

[5] Sahota PS, Burn JL, Heaton M, et al. Development of a reconstructed human skin model for angiogenesis
[J]. Wound Repair Regen, 2003, 11(4): 275-284.

[6] Wenger A, Stahl A, Weber H, et al. Modulation of in vitro angiogenesis in a three-dimensional spheroidal coculture model for bone tissue engineering
[J]. Tissue Eng, 2004, 10(9-10): 1536-1547.

[7] Suh S, Kim J, Shin J, Kil K. Use of omentum as an in vivo cell culture system in tissue engineering
[J]. ASAIO J, 2004, 50(5): 464-467.

[8] Patrick CW Jr, Chauvin PB, Hobley J. Preadipocyte seeded PLGA scaffolds for adipose tissue engineering
[J]. Tissue Eng, 1999,5(2): 139-151.

[9] Philippe P, Franck Villars, Simone MP. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats
[J]. Plast Res Surg,2003,111(6): 1932-1941.

[10]Neumann T, Nicholson BS, Sanders JE. Tissue engineering of perfused microvessels
[J]. Microvasc Res,2003,66(1): 59-67.

[11]王学明, 裴国献. 组织工程骨血管化的监测
[J]. 中华创伤骨科, 2004, 6(7): 822-824.

[12]Ribatti D, Nico B, Vacca A, et al. Chorioallantoic membrane capillary bed: a useful target for studying angiogenesis and antiangiogenesis in vivo
[J]. Anat Rec, 2001, 264 (4):317-324.

[13]Borges J, Tegtmeier FT, Padron NT, et al. Chorioallantoic membrane angiogenesis model for tissue engineering: A new twist on a classic model
[J]. Tissue Eng, 2003,9(3):441-450.

[1]	郭红志, 王瑜, 张加琪, 梁海彬, 马梓玮, 冯伟, 吴忧, 司子燚. 血管铸型结合CT三维模型评估先兆子痫胎盘血管结构[J]. 中国临床解剖学杂志, 2024, 42(1): 5-10.
[2]	陈俊臣, 曹文英, 罗燕君, 何婉仪, 朱真真, 彭志强. 抽吸取栓术与抽吸结合支架取栓术治疗脑前循环栓塞疗效比较[J]. 中国临床解剖学杂志, 2024, 42(1): 89-93.
[3]	丁冉, 郑雪峰, 丁自海, 吴涛. 胆囊静脉是如何回流的？ ——临床解剖学研究[J]. 中国临床解剖学杂志, 2023, 41(3): 249-253.
[4]	于洋, 宋永才, 杨立峰. 复合骨碎补总黄酮的丝素蛋白/壳聚糖支架在兔关节软骨损伤中的应用研究[J]. 中国临床解剖学杂志, 2022, 40(6): 696-703.
[5]	马洪峰, 任庆, 郑末, 程万民, 廉梅. 脂肪间充质干细胞聚合物-细胞外基质复合物补片修复兔外耳道全层皮肤损伤的实验研究[J]. 中国临床解剖学杂志, 2022, 40(6): 704-709.
[6]	刘丹,杜奥玲,王新旺,张德明,唐汇李,王宇航,陈胜华. 京尼平交联大鼠肾去细胞生物支架提高支架生物学性能的实验研究[J]. 中国临床解剖学杂志, 2019, 37(1): 71-76.
[7]	肖钊明,李泽宇,刘畅,马梓玮,吴坤成,许广威,梁海彬,骆宝华,骆世文,骆建恒,戴景兴,欧阳钧 . 利用微血管铸型技术研究肝外胆管动脉的分布特征[J]. 中国临床解剖学杂志, 2018, 36(6): 601-605.
[8]	赖秋华，　张洁馨，　周晓晗，　王雅洁，　李烨，　刘阳，　毕振宇，　欧阳钧. 牙周炎大鼠毛细血管铸型构建[J]. 中国临床解剖学杂志, 2018, 36(4): 423-425.
[9]	黄世嘉,吴威乐,陈小星,王乐禹,刘成龙. 导电弹性水凝胶促进C2C12细胞增殖的实验研究[J]. 中国临床解剖学杂志, 2018, 36(3): 277-281.
[10]	王新旺,周乐斌,梅劲,张德明,刘丹,杜奥玲,陈胜华. 猪兔鼠肾生物支架制备及体外细胞共培养探究[J]. 中国临床解剖学杂志, 2018, 36(2): 174-181.
[11]	刘洁,徐佰达,凌秋洋,王霄,安冬梅,曹丽,白元,宗刚军. 经皮“瓣上瓣”支架植入治疗急性主动脉瓣关闭不全的应用解剖[J]. 中国临床解剖学杂志, 2016, 34(5): 481-485.
[12]	毕振宇,刘阳,孙培栋,杨宇超,欧阳钧. Wistar大鼠口腔颌面部微血管铸型制作[J]. 中国临床解剖学杂志, 2015, 33(5): 604-607.
[13]	李文春，　李静，　唐杰，　张亮，　王飞虎，　陈秀英. 猪胆总管脱细胞基质构建组织工程胆管的生物力学评价[J]. 中国临床解剖学杂志, 2015, 33(5): 553-557.
[14]	李庆庆,王大平,熊建义,黄江鸿,付志杰. Runx2重组慢病毒感染骨髓间充质干细胞并促进其成骨分化的研究[J]. 中国临床解剖学杂志, 2015, 33(3): 311-315.
[15]	杨联军, 荣子杰, 张赞杰, 伍国峰, 张西兵, 王健, 朱立新. 载ADM-PLGA纳米微球的ADM-PLGA-NHAC制备及其对人骨肉瘤MG63细胞的抑制作用[J]. 中国临床解剖学杂志, 2014, 32(5): 585-589.