可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的生物相容性实验研究

中国临床解剖学杂志 ›› 2011, Vol. 29 ›› Issue (6): 686-689.

可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的生物相容性实验研究

陈彦¹，　于　博²，　李松建²，　陈　仲²，　刘成龙²

南方医科大学附属珠江医院 1.超声科, 2.骨科，广州 510282

收稿日期:2011-05-03 出版日期:2011-11-25 发布日期:2011-12-12
通讯作者: 李松建，副主任医师，副教授，E-mail: lisongjian321@126.　com E-mail:gzyubo@gmail.com
作者简介:陈　彦(1977-)，女，北京人，主治医师，研究方向：组织工程，超声医学
基金资助:
广东省自然科学基金（10451051501004727），国家自然科学基金（81101348）

The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold

CHEN Yan¹, YU Bo ², LI Song-jian²,CHEN Zhong², LIU Cheng-long²

1. Department of Ultrasonic Diagnosis; 2. Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

Received:2011-05-03 Online:2011-11-25 Published:2011-12-12

摘要/Abstract

摘要：

目的　评价可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的组织相容性，以及探讨材料负载骨髓基质干细胞进行骨修复的可行性。方法　壳聚糖/纳米羟基磷灰石/胶原材料与BrdU标记的rBMSc混合，注射入大鼠皮下，于皮下植入后24 h，14 d和28 d分别断颈处死大鼠，进行大体观察、HE、MASSON染色、免疫组化染色。结果　壳聚糖/纳米羟基磷灰石/胶原材料在室温呈液态，经1 ml注射器26号针头注射入大鼠皮下后，材料在体内可以迅速原位成形成凝胶状态，并在原位保持形状，BrdU标记的rBMSc弥散于材料中，28 d的免疫组化结果显示细胞存活良好。结论　壳聚糖/纳米羟基磷灰石/胶原复合材料具有良好的组织相容性，是一种良好的负载干细胞的骨组织工程支架。

关键词: , 可注射支架, 组织工程, 生物相容性

Abstract:

Objective　To evaluate the biocompatibility of an injectable chitosan (CS) /nano-hydroxyapatite(HA)/collagen (Col) scaffold.　Methods　rBMSCs was mixed with CS/HA/Col solution and subcutaneously injected into Wistar rats. After 24h, 14d and 28d, the animals were sacrificed and subcutaneous tissues at injecting site were removed. HE, MASSON and immunohistochemical stainings were performed for evaluating the features if scaffolds.　Results　The liquid CS/HA/Col (room temperature) would form the gel after injecting into the subcutaneous tissue. The mixed rBMSCs survived well in the CS/HA/Col gel at even 28 days in vivo. The CS/HA/Col/rBMSCs gel induced less inflammatory reaction in the host tissue compared to that of simple CS/HA/Col gel.　Conclusions　Our results suggest that the CS/HA/Col system can be used to load rBMSCs in vitro homogeneously. The CS/HA/Col system can be used in vivo with the minimally invasive manner and feasible biocompatible environment.

Key words: Injectable scaffold, Tissue engineering, Biocompatibility

中图分类号:

R318.08

陈彦, 于博, 李松建, 陈仲, 刘成龙. 可注射壳聚糖/纳米羟基磷灰石/胶原复合材料的生物相容性实验研究[J]. 中国临床解剖学杂志, 2011, 29(6): 686-689.

CHEN Pan, XU Bo, LI Song-Jian, CHEN Zhong, LIU Cheng-Long. The biocompatibility of the injectable chitosan/nano-hydroxyapatite/ collagen scaffold[J]. Chinese Journal Of Clinical Anatomy, 2011, 29(6): 686-689.

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