CK19和PCNA在肝纤维化大鼠部分肝切除后再生肝中的表达

中国临床解剖学杂志 ›› 2012, Vol. 30 ›› Issue (5): 552-555.

CK19和PCNA在肝纤维化大鼠部分肝切除后再生肝中的表达

方方^1,2，　刘前进³，　岳学强^1,2，　李娜娜²，　刘恒兴^1,2

1.新乡医学院人体解剖学教研室, 新乡 453003; 2.河南省医用组织再生重点实验室, 新乡 453003;
3.永城市人民医院, 商丘 476600

收稿日期:2012-02-29 出版日期:2012-09-25 发布日期:2012-09-29
通讯作者: 刘恒兴，教授，E-mail: liuhengxing@hotmail.com
作者简介:方方（1984-），女，河南省新乡市人，硕士，助教，主要从事肝纤维化肝再生研究
基金资助:
新乡医学院重点学科开放课题(ZD200949)

Expression of CK19 and PCNA in fibrosis rat regenerating liver after partial hepatectomy

FANG Fang1, 2, LIU Qian-jin 2,3, YUE Xue-qiang1,2, LI Na-na 1,2, LIU Heng-xing 1,2

1.Department of Anatomy, Xinxiang Medical college, Xinxiang 453003, China; 2.Henan Medical Tissue Regeneration Key Laboratory, Xinxiang 453000, China; 3.Yongcheng People's Hospital, Shangqiu 476600, China

Received:2012-02-29 Online:2012-09-25 Published:2012-09-29

摘要/Abstract

摘要：

目的　检测肝纤维化大鼠部分肝切除后不同时点CK19及PCNA的表达，了解胆管再生情况。方法　雄性SD大鼠，对照组和实验组各42只，实验组腹腔注射CCl4制备肝纤维化模型，两组均进行部分肝切除术，在不同时点取材后利用HE、免疫组化及免疫荧光双标染色等方法检测CK19和PCNA的表达情况。结果　实验组和对照组术后随时间延长CK19表达均呈增强趋势，且实验组术后各时间点CK19的表达均高于对照组同时间点。两组PCNA的表达量都随时间推移逐步升高，但实验组大鼠明显上升缓慢，持续时间延长，表达高峰晚于对照组出现。结论　（1）肝纤维化大鼠部分肝切除能刺激肝卵圆细胞增殖和向胆管细胞的分化，而正常大鼠部分肝切除后再生肝中的胆管上皮细胞主要来源于原有细胞的代偿性增生。（2）术前肝纤维化大鼠的肝脏细胞就出现了增殖修复，术后由于肝纤维化大鼠本身肝脏受到伤害，因此肝脏的有效再生细胞数低于正常肝脏。

关键词: 大鼠, 肝纤维化, 胆管, CK19, PCNA

Abstract:

Objective　To detect the expressions of CK19 and PCNA in fibrosis rat regenerating liver after partial hepatectomy (PH) at the different time phase, for evaluating the bile duct regeneration in fibrotic liver.　Methods　Male SD rats were divided into two groups: the control group (42 rats) and the experimental group (42 rats). The rats of experimental group were injected CCl4 to establish the hepatic fibrosis model, and then the two groups was endured PH operation. HE, immunohistochemical and double-label immunofluorescence were used to detect and analyze expressions of CK19 and PCNA in regenerating liver.　Results　The expression of CK19 increased gradually after PH in both groups, however, expression intensity in experimental animals was significantly higher than control ones at the same time point. As well, the expression of PCNA increased gradually after PH in both groups, however, expression level in experimental groups rose slowly, extended and the peak appeared later than the control.　Conclusions　(1) PH process of fibrotic rat liver can stimulate proliferation of OCs and the differentiation to BECs, which mainly originate from compensatory hyperplasia in normal rats regenerating liver. (2) Before PH, liver cells in fibrosis rats have already proliferated, after PH, the effective regenerating cells are lower than the other liver injury.

Key words: Rat, Hepatic fibrosis, Bile duct, CK19, PCNA

中图分类号:

R575

方方, 刘前进, 岳学强, 李娜娜, 刘恒兴. CK19和PCNA在肝纤维化大鼠部分肝切除后再生肝中的表达[J]. 中国临床解剖学杂志, 2012, 30(5): 552-555.

FANG Fang, LIU Jian-Jin, YUE Hua-Jiang, LI Na-Na, LIU Heng-Xin. Expression of CK19 and PCNA in fibrosis rat regenerating liver after partial hepatectomy[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(5): 552-555.

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