茶多酚预防慢性酒精中毒对精子损伤的作用及机制研究

doi:10.13418/j.issn.1001-165x.2014.05.022

中国临床解剖学杂志 ›› 2014, Vol. 32 ›› Issue (5): 604-608.doi: 10.13418/j.issn.1001-165x.2014.05.022

茶多酚预防慢性酒精中毒对精子损伤的作用及机制研究

汤银娟^1,2,　王建钧³,　王祥海⁴,　文锦坤⁴,　潘梦婕⁴,　钱长晖⁴,　康伟祥³,　陈卓³

1.湘南学院组织胚胎学教研室，湖南郴州 423000; 2.南岭药用资源研究所，湖南郴州 423000;
3.湘南学院临床医学系，湖南郴州 423000； 4.南方医科大学组织胚胎学教研室，广州 510515

收稿日期:2014-05-20 出版日期:2014-09-25 发布日期:2014-10-14
通讯作者: 王建钧, 讲师,E-mail:6365294@qq.com E-mail:vigor1980@126.com
作者简介:汤银娟（1980-），女, 湖南湘潭人，讲师，在读博士，主要从事环境与生殖方面的研究
基金资助:
湖南省教育厅课题（11C1177）；湘南学院大学生研究性学习与创新性实验项目（2012-22）；湘南学院科研课题（2011YY49）

Tea polyphenol alleviates the chronic alcoholism induced sperm damage

TANG Yin-juan ^1,2, WANG Jian-jun ³, WANG Xiang-hai⁴, WEN Jin-kun⁴, PAN Meng-jie ⁴, QIAN Chang-hui ⁴, KANG Wei-xiang³, CHEN Zhuo³

1.Department of Histology and Embryology, Xiangnan University, Chenzhou 423000; 2. Institute of Nanling Medical Resources, Xiangnan University, Chenzhou 423000; 3. Department of Clinical Medicine, Xiangnan University, Chenzhou 423000; 4. Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China

Received:2014-05-20 Online:2014-09-25 Published:2014-10-14

摘要/Abstract

摘要：

目的　探讨茶多酚预防慢性酒精中毒对精子损伤的作用及其可能机制。方法　40只雄性小鼠随机分为4组：酒精损伤组（CA组）、茶多酚低剂量组（TP-L组）及茶多酚高剂量组（TP-H组）每天先喂服酒精，8 h后再分别喂服纯水、或浓度为1%或2%的茶多酚悬液。正常对照组（NC组）以同样方式和剂量每天2次喂服纯水。90 d后取材制备精子悬液用于精子密度、精子活动率及畸形率的检测。同时，取睾丸组织行免疫组织化学检测Bcl-2和Bax的表达。结果　与NC组比较, CA组的精子密度和精子活动率明显下降，精子畸形率明显增加。相对于CA组,TP-L组及TP-H组精子密度和精子活动率增高, 精子畸形率降低，其中TP-H组的效果比TP-L组更显著。CA组睾丸组织中Bcl-2的阳性细胞比NC组显著减少，Bax的阳性率却明显升高。喂服茶多酚却能明显逆转由酒精导致的上述变化。结论　茶多酚能预防酒精对精子的损伤,这可能与精子发生过程Bcl-2及Bax的表达变化有关。

关键词: 茶多酚, 酒精中毒, 精子, 睾丸, Bcl-2, Bax

Abstract:

Objective　To explore the preventive role of tea polyphenol (TP) on the chronic alcoholism induced sperm damage and the potential mechanisms.　Methods　Forty male Kunming mice were randomly divided into chronic alcoholism (CA) group, TP low-dosage (TP-L) group, TP high-dosage (TP-H) group and normal control (NC) group. Eight hours after daily alcohol administration, the mice in CA, TP-L and TP-H groups were treated with water, 1% TP or 2% TP respectively. The mice in NC group were administered with water twice a day. 90 days later, sperm suspension was prepared to detect sperm density, activity ratio and malformation ratio; Bcl-2 and Bax expression were observed in testicular tissue via immunohistochemistry.　Results　In CA group, the density, activity ratio of sperm and ratio of Bcl-2 positive cells in testicular tissue were lower, the ratios of sperm malformation and Bax positive cells in testicular tissue were higher than that of NC group. Above changes were reversed in the TP-L and TP-H groups. Moreover, this benign effect showed a positive correlation with dosage.　Conclusion　Tea ployphenol could alleviate alcoholism induced sperm damage. This effect might relate with the Bcl-2 and Bax expression during spermatogenesis.

Key words: Tea polyphenols, Alcoholism, Sperm, Testis, Bcl-2, Bax

中图分类号:

R285.5

汤银娟, 王建钧, 王祥海, 文锦坤, 潘梦婕, 钱长晖, 康伟祥, 陈卓. 茶多酚预防慢性酒精中毒对精子损伤的作用及机制研究[J]. 中国临床解剖学杂志, 2014, 32(5): 604-608.

SHANG Yin-Juan, WANG Jian-Jun, WANG Xiang-Hai, WEN Jin-Kun, BO Meng-Jie, JIAN Chang-Hui, KANG Wei-Xiang, CHEN Zhuo. Tea polyphenol alleviates the chronic alcoholism induced sperm damage[J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 604-608.

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茶多酚预防慢性酒精中毒对精子损伤的作用及机制研究

Tea polyphenol alleviates the chronic alcoholism induced sperm damage

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