关键词: 视觉剥夺, 视网膜, 蛋白组学

Abstract:

Objective   To explore the influence of visual deprivation on retinal protein expression. Methods   The visual deprivation model is established by bilateral eyelid closure. Bilateral eyelid closure was performed at the postnatal 14 days, 2 weeks after which the rats were used for further experiment  Two-dimensional gel electrophoresis was used to separate proteins in the retina, the differential proteins were chosen using PDQuest software and identified by tandem MS. the change of protein expression was further confirmed by Western blot and immunohistochemistry. Hematoxylin and eosin staining was performed to observe the retinal structure.   Results   Expressions of four proteins in the rat retina were decreased after visual deprivation. These proteins were identified as heat shock 70、alpha-enolase、serotranfferin and recoverin. However, we did not observe the change of the amoun of the retinal cells under the light microscope.  Conclusions   Short-term visual deprivation induces the change of retinal proteins, and may contribute to the occurrence of amblyopia.

Key words: Visual deprivation, Retina, Proteomics