关键词: 脑梗死, 急性期高血糖, TTC染色, 改良神经功能缺损评分, 神经干细胞

Abstract:

Objective   To explore the correlation between non-diabetic cerebral infarction and acute hyperglycemia and its possible mechanism. Methods   In this experiment ,  twenty-four SD rats were randomly divided into three groups, normoglycemia group (NG), two hyperglycemia groups(HG1 and HG2). Rats were rendered acute hyperglycemia during occluded the middle cerebral artery (MCAO) .To assess the correlation between non-diabetic cerebral infarction and acute hyperglycemia , the rats were observed for neurological functional impairment scoring and cerebral infarct volumes at 24 h after cerebral infarction. At the same time, the proliferation capacity of neural stem cell in ischemic ipsilateral dentate gyrus was observed.  Results   The levels of blood glucose of NG group was 4.0~5.0 mmol/L，and HG1 and HG2 groups were markedly up to the target blood glucose concentrations of 10.0 mmol/L and 20.0 mmol/L, respectively（P<0.01）. 24h after MCAO, cerebral infarct volumes of HG2 group were obviously more larger than NG group and HG1 group (P<0.05), which suffered from more severe neurological impairment (P<0.05). However, there were no significant difference in cerebral infarct volumes and neurological deficits between HG1 group and NG group (P>0.05). The proliferation capacity of neural stem cells detection results were consistent with that of neurological deficits and cerebral infarct volumes.  Conclusion   Mild acute hyperglycemia (10.0 mmol/L) may protect the neural stem cells against the infarction cerebral injury. Severe hyperglycemia (20 mmol/L) obviously deteriorated infarct brain injury, which may be associated with impaired proliferation capacity of neural stem cells.

Key words: Cerebral infarction, Acute hyperglycemia, TTC, Zea longa score, Neural stem cells