改良人颅咽管瘤原代细胞培养方法

doi:10.13418/j.issn.1001-165x.2015.03.021

中国临床解剖学杂志 ›› 2015, Vol. 33 ›› Issue (3): 331-335.doi: 10.13418/j.issn.1001-165x.2015.03.021

改良人颅咽管瘤原代细胞培养方法

陈铭，　刘忆，　潘军，　石瑾，　万仁宽，　汪潮湖，　宋烨，　漆松涛

南方医科大学南方医院神经外科，广州 510515

收稿日期:2014-08-21 出版日期:2015-05-25 发布日期:2015-07-24
通讯作者: 漆松涛，教授，博士生导师，E-mail: qisongtaosjwk@163.com
作者简介:陈铭（1989-），男，上海人，临床医学八年制在读，主要从事神经外科基础及临床研究，E-mail: chenming7020@gmail.com
基金资助:
广东省自然科学基金（S2011010003063）；广东省自然科学基金（S2011020005179）

The improved primary cell culture method of craniopharyngioma

CHEN Ming, LIU Yi, PAN Jun, SHI Jin, WAN Ren-kuan, WANG Chao-hu, SONG Ye, QI Song-tao

Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

Received:2014-08-21 Online:2015-05-25 Published:2015-07-24

摘要/Abstract

摘要：

目的　探索一种改良人颅咽管瘤细胞培养方法。方法　根据文献报道和自己的经验，改良既往文献报道的颅咽管瘤细胞培养方法，对两种病理类型的人颅咽管瘤细胞进行原代培养，同时对其进行纯化与传代。通过观察细胞镜下形态、细胞免疫组织化学染色以及细胞增殖实验对原代细胞进行鉴定。结果　改良后的培养方法培养出的颅咽管瘤细胞从组织块中爬出，排列紧密，透亮且折光性强。细胞免疫组化显示，两种病理类型的细胞均为颅咽管瘤细胞。增殖实验显示，细胞在体外仍具有较好的增殖和分裂的能力。结论　本研究改良了人颅咽管瘤原代细胞的培养方法，具有简单、经济、成功率高等特点,为颅咽管瘤基础研究提供了良好的保证。

关键词: 颅咽管瘤, 改良, 细胞培养

Abstract:

Objective Using new method to culture the craniopharyngioma cells in order to provide the foundation of clinical and basic researches on cell level. Methods Using new method to culture, purify and pass two pathological patterns of craniopharyngioma cells. The cells were identified by morphology under microscope, immunohistochemistry and proliferation assay. Results The craniopharyngioma cells cultured by the new method were seen around the tumor piece with better state and greater numbers than the cells cultured by the traditional method. These two kinds of cells were determined to be the craniopharyngioma cells. The proliferation assay showed that the cells were still proliferating and dividing in vitro. Conclusions Using new method which was simple and cheap to culture the craniopharyngioma cells could harvest better cells in state and greater in numbers. Though this method could not solve all the problems about cell culture of craniopharyngioma, it could still provide the foundation of clinical and basic researches about tumor biological characteristics on the cell level by establishment of a stable cell line.

Key words: Craniopharyngioma, Primary culture, Cell line

陈铭,刘忆,潘军,石瑾,万仁宽,汪潮湖,宋烨,漆松涛. 改良人颅咽管瘤原代细胞培养方法[J]. 中国临床解剖学杂志, 2015, 33(3): 331-335.

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改良人颅咽管瘤原代细胞培养方法

The improved primary cell culture method of craniopharyngioma

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