关键词: 种植体支抗, 基质金属蛋白酶-2, 骨改建

Abstract:

Objective　To observe the expression of matrix metalloproteinase 2 in the periodontal tissues with implant anchorage for the anterior tooth in dogs and to explore its role in the bone remodeling.  Methods　The experimental model was established by pressing down the anterior tooth with implant anchorage. After imposing 100g  force, the dogs were executed sequentially  at  6，12，24，36 weeks.  The right second maxilla incisor with gum and the integral alveolar bone in the affected side were cut out for observation of general morphology by HE staining and detection of  the change of expression and time distribution characteristics of matrix metallopmteinase 2(MMP-2) by immunohistochemistry. The left side without force imposed is chosen as the control group, and the tissue surrounding the right second maxilla incisor was explored by immunohistochemicaI detection and HE staining.　 Results　It showed that there is a large number of bone in the tooth root apex of the control group by HE staining. However, it manifested bone absorption in the tooth root apex with force applied. The expression of MMP-2 in the periodontal tissue raised at 6 weeks, peaked at 24 weeks, and then began to decline afterwards. the result showed that difference was statistically significant after application of force by immunohistochemistry (P<0.05).　Conclusions　In the process of pressing down the incisor, MMP-2 plays the important role in the reconstruction of periodontal tissue.

Key words:  ImpIant anchorage, Matrix metalloproteinase 2, Bone remodeling