糖尿病神经病理性疼痛大鼠脑异常活动的功能磁共振研究

doi:10.13418/j.issn.1001-165x.2016.04.011

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (4): 407-411.doi: 10.13418/j.issn.1001-165x.2016.04.011

糖尿病神经病理性疼痛大鼠脑异常活动的功能磁共振研究

吴柏霖¹，　法志强¹，　赖春任²，　郭圣文²，　郭燕舞¹

1. 南方医科大学珠江医院神经外科, 国家临床重点专科，教育部工程技术研究中心, 广东省脑功能修复与再生
重点实验室, 广州 510282; 2.华南理工大学材料科学与工程学院生物医学工程系，广州 510006

收稿日期:2016-03-24 出版日期:2016-07-25 发布日期:2016-08-03
通讯作者: 郭燕舞，副主任医师，硕士生导师，E-mail:eguoyanwu @163.com
作者简介:吴柏霖（1989-），男，广东化州人，在读硕士，主要从事神经病理性疼痛的相关研究，Tel：13268269144，E-mail:ewubailin@163.com
基金资助:
国家自然科学基金（31300921）；广东省自然科学基金（S2013040016710）；广东省科技计划项目基金(2014A020212179)

Abnormal activity of brain detected by functional magnetic resonance imaging in a rat model of diabetic neuropathic pain

WU Bo-lin¹, FA Zhi-qiang¹, LAI Chun-ren², GUO Sheng-wen², GUO Yan-wu¹

1.The National Key Clinical Specialty, The Engineering Technology Research Center of Education Ministry of China, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China; 2. Department of Biomedical Engineering, School of Material Science and Engineering, South China University of Technology, Guangzhou 510006, China

Received:2016-03-24 Online:2016-07-25 Published:2016-08-03
Contact: GUO Yan-wu, E-mail:eguoyanwu @163.com

摘要/Abstract

摘要：

目的　构建糖尿病神经病理性疼痛大鼠模型，利用功能磁共振成像技术，观察脑功能活动变化情况，探索异常功能活动的脑区。方法　雄性SD大鼠48只，随机分为糖尿病组（n=36）和对照组（n=12）。糖尿病组通过腹腔注射60 mg/kg链脲佐菌素构建糖尿病模型，同时测定50％机械刺激缩足反射阈值的变化情况，根据触觉诱发性疼痛标准，糖尿病组大鼠被分成疼痛组及无痛组。利用锰离子增强功能磁共振成像技术进行大鼠脑功能成像，图像后处理分析糖尿病神经病理性疼痛大鼠脑内异常功能活动区域。结果　成功构建糖尿病神经病理性疼痛大鼠模型，发现脑内存在显著性功能活动增强的区域，包括感觉皮层、腹内侧前额叶皮层、前扣带皮层、下橄榄核、梨状皮层、杏仁核及岛叶部分皮层。结论　糖尿病神经病理性疼痛大鼠脑内部分脑区具有异常功能活动的表现，这些脑区很有可能参与该疼痛调控的中枢机制。

关键词: 糖尿病, 疼痛, 功能磁共振成像, 异常功能活动, 大脑

Abstract:

Objective　To construct the diabetic neuropathic pain rat model. To detect the functional activity changes of brain and explore the brain regions with abnormal function activities using functional magnetic resonance imaging. Methods Forty-eight male SD rats were chosen and randomly divided into two groups: a diabetes group (n=36) and a control group (n=12). Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg) with observation of the changes of fasting blood glucose level and body weight. Behavioral testing for paw 50% withdrawal threshold was performed at the same time. Tactile allodynia was defined as a 50% withdrawal threshold of less than or equal to 2.0 g. According to the tactile allodynia, the diabetes group was sorted into two groups: the pain group and painless group. Functional activity mapping of brain by manganese enhanced magnetic resonance imaging and voxel-wise t-test were then applied to statistically analyze the brain regions with abnormal function activities in diabetic neuropathic pain rat. Results Diabetic neuropathic pain rat was successfully induced. some brain regions, including the somatosensory cortex, ventromedial prefrontal cortex, anterior cingulate cortex, inferior olive, piriform cortex,and part of the cortexes of the amygdala and insula, were found with significantly enhanced activity in diabetic neuropathic pain rat. Conclusions The results suggest these brain regions with abnormal function activities probably take part in the regulation of diabetic neuropathic pain within central nervous system.

Key words: Diabetes, Pain, Functional magnetic resonance imaging, Abnormal function activity, Brain

吴柏霖,法志强,赖春任,郭圣文,郭燕舞. 糖尿病神经病理性疼痛大鼠脑异常活动的功能磁共振研究[J]. 中国临床解剖学杂志, 2016, 34(4): 407-411.

WU Bo-lin, FA Zhi-qiang, LAI Chun-ren, GUO Sheng-wen, GUO Yan-wu. Abnormal activity of brain detected by functional magnetic resonance imaging in a rat model of diabetic neuropathic pain[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(4): 407-411.

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糖尿病神经病理性疼痛大鼠脑异常活动的功能磁共振研究

Abnormal activity of brain detected by functional magnetic resonance imaging in a rat model of diabetic neuropathic pain

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