DWI检测超急性期兔大脑中动脉梗死模型神经血管单元损伤的初步研究

doi:10.13418/j.issn.1001-165x.2016.05.013

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (5): 540-545.doi: 10.13418/j.issn.1001-165x.2016.05.013

DWI检测超急性期兔大脑中动脉梗死模型神经血管单元损伤的初步研究

彭松¹，　何炜²，　叶斌¹，　容鹏飞¹，　梁琪¹，　张声旺¹，　廖云杰¹，　胡鹏志¹

中南大学湘雅三医院　1.放射科, 2.超声科，长沙 410013

收稿日期:2016-04-11 出版日期:2016-09-25 发布日期:2016-10-14
通讯作者: 胡鹏志，副主任技师，Tel：(0731)88618415，E-mail：pengxiang15@vip.sina.com
作者简介:彭松（1981-），男，土家族，湖南省龙山县人，医学硕士，主管技师，主要从事医学影像技术方向的研究，Tel：(0731)88618412,E-mail：pengsong@csu.edu.cn
基金资助:
湖南省科学计划项目(2012FJ4309)

The preliminary research on neurovascular unit injury of hyperacute infarction of rabbit middle cerebral artery by magnetic resonance diffusion-weighted imaging

PENG Song¹, HE Wei², YE Bin¹, RONG Peng-fei¹, LIANG Qi¹, ZHANG Sheng-wang¹, LIAO Yun-jie¹, HU Peng-zhi¹

1.Department of Radiology; 2. Department of Ultrasonography, Third Xiangya Hospital of Central South University, Changsha 410013, China

Received:2016-04-11 Online:2016-09-25 Published:2016-10-14

摘要/Abstract

摘要：

目的　评价磁共振弥散加权成像（diffused weighted imaging, DWI）对兔脑超急性期脑梗死模型中神经血管单元（neurovascular unit , NVU）损伤的诊断价值。方法　15只健康新西兰大白兔，随机分为对照组（5只）和实验组（10只）。其中实验组用微导丝超选择性栓塞法制作兔大脑中动脉阻塞(middle cerebral artery occlusion, MCAO)模型，并根据存活时间分为0.5 h和4 h组。所有动物行磁共振DWI扫描和T2-flair扫描并分析表观弥散系数（apparent diffusion coefficient，ADC）和相对表观弥散系数（relative apparent diffusion coefficient，rADC），之后立即取全脑固定，切片进行尼氏染色、GFAP（glial fibrillary acidic protein，星形胶质细胞标记物）和CD31（血管内皮标记物）染色。结果　和对照组相比， 0.5 h组缺血区DWI成像见高信号，ADC成像见低信号，T2-Flair成像未见异常信号，尼氏染色未见异常，GFAP染色表达增加；4h组缺血区DWI成像见高信号，ADC成像见低信号，二者成像范围和强度较0.5h组扩大增强，T2-Flair成像见高信号，尼氏染色见尼氏体数目显著减少，GFAP染色表达较0.5h组显著增加。与0.5h组比，4h组缺血区的ADC值及rADC值均有显著差异（P<0.01）。实验组rADC值与GFAP荧光强度值呈负直线相关关系（r=－0.672，P<0.001）。结论　DWI对超急性期兔脑梗死可以及时准确的进行诊断，对应时间点梗死模型的NVU损伤特点符合影像学特征。

关键词: , DWI, 兔, MCAO模型, 神经血管单元

Abstract:

Objective　To investigate the diagnostic efficacy of magnetic resonance diffusion-weighted imaging (DWI) on the injury characteristics of neurovascular unit (NVU) of hyperacute infarction of rabbit brain.　Methods　Fifteen New Zealand rabbits were randomly separated into two groups: the contrast group (n=5) and the experimental group (n=10). Micro-catheter was used to produce middle cerebral artery occlusion (MCAO) on the experimental group animals via internal carotid artery. According to different occlusion time, the experimental group was divided into 2 sub-groups (sub-group 0.5 h and 4 h) with 5 animals per group. All the animals underwent T2-flair and DWI scanning before and after MCAO. The imaging characteristics of DWI and the values of apparent diffusion coefficient (ADC) and relative apparent diffusion coefficient (rADC) were analyzed. After MRI, the animals were sacrificed for the Nissl′s staining, the glial fibrillary acidic protein (GFAP) and CD31 detection of brains.　Results　Compared with the contrast group, abnormal high intensity signal on DWI, low signal on ADC, unaltered T2-flair signal, high expression of GFAP were observed at sub-group 0.5 h in the right basal ganglia region. At sub-group 4 h, abnormal high intensity signal on DWI and low signal on ADC which appeared more intense than sub-group 0.5 h. T2-flair scanning showed high signal. The Nissl’s results showed that the number of Nissl’s body at the control group and sub-group 0.5 h appeared intact, while the number of Nissl’s body decreased and neurons were damaged at sub-group 4 h. The high expression of GFAP were observed. The values of ADC and rADC in sub-group 0.5 h were significantly different with those in sub-group 4 h. The value of rADC and the intensity of GFAP showed linear correlation (r=-0.672, P<0.01).　Conclusions　The DWI can diagnose accurately on hyperacute infarction of rabbit brain, and the DWI changes of relative time were consistent with the injury of NVU.

Key words: Diffusion-weighted imaging, Rabbit, Middle cerebral artery occlusion, Neurovascular unit

彭松,何炜,叶斌,容鹏飞,梁琪,张声旺,廖云杰,胡鹏志. DWI检测超急性期兔大脑中动脉梗死模型神经血管单元损伤的初步研究[J]. 中国临床解剖学杂志, 2016, 34(5): 540-545.

PENG Song, HE Wei, YE Bin, RONG Peng-fei, LIANG Qi, ZHANG Sheng-wang, LIAO Yun-jie, HU Peng-zhi. The preliminary research on neurovascular unit injury of hyperacute infarction of rabbit middle cerebral artery by magnetic resonance diffusion-weighted imaging[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 540-545.

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DWI检测超急性期兔大脑中动脉梗死模型神经血管单元损伤的初步研究

The preliminary research on neurovascular unit injury of hyperacute infarction of rabbit middle cerebral artery by magnetic resonance diffusion-weighted imaging

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