人脂肪源干细胞促进前交叉韧带重建后腱骨界面愈合的实验研究

doi:10.13418/j.issn.1001-165x.2016.06.010

中国临床解剖学杂志 ›› 2016, Vol. 34 ›› Issue (6): 643-646.doi: 10.13418/j.issn.1001-165x.2016.06.010

人脂肪源干细胞促进前交叉韧带重建后腱骨界面愈合的实验研究

陈平¹，　曲戎梅²，　韩忠宇¹，　杨宇超²，　尹玉琨¹，　田京¹，　张力¹

1.南方医科大学珠江医院骨科，广州 510282；　2.南方医科大学基础医学院人体解剖学教研室，广州 510515

收稿日期:2016-07-19 出版日期:2016-11-25 发布日期:2016-12-20
通讯作者: 田京,教授，硕士生导师，E-mail:tianjing6723@aliyun.com.cn；张力, 副教授，副主任医师，E-mail: tension_zh@163.com.cn
作者简介:陈平（1990-），男，江西贵溪人，在读硕士，主要从事关节外科方面研究, Tel：（020）62782572，E-mail:chen_ping2016@163.com
基金资助:
广东省科技计划（2013B021800150）

The study of human adipose-derived stem cells in promoting the tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model

CHEN Ping¹, QU Rong-mei², HAN Zhong-yu¹, YANG Yu-chao², YIN Yu-kun¹, TIAN Jing¹, ZHANG Li¹

1.Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China; 2.Department of Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China

Received:2016-07-19 Online:2016-11-25 Published:2016-12-20
Contact: TIAN Jing, E-mail:tianjing6723@aliyun.com.cn; ZHANG Li, E-mail:tension _zh@163.com

摘要/Abstract

摘要：

目的　探讨人脂肪源干细胞（human adipose-derived stem cells, hASCs）能否促进前交叉韧带重建后腱骨界面的愈合。方法　将30只体重2.0~2.5 kg的雌性新西兰大白兔随机分为实验组和对照组，每组各15只。取同侧半腱肌作为供体肌腱，建立单侧下肢前交叉韧带重建动物模型，实验组和对照分别在胫骨和股骨隧道中注射hASCs或等量的生理盐水。分别在术后2、4和8周取材，收集胫骨-移植韧带-股骨标本行大体观察和组织学观察。结果　HE染色示对照组术后2周腱骨界面主要以炎性细胞浸润为主，4周后炎性细胞减少，8周时腱骨界面可见Sharpey纤维，形成间接止点。实验组术后4周腱骨界面可见纤维软骨细胞及少许骨细胞形成，术后8周可见新骨形成,形成直接止点的结构。结论　hASCs能促进前交叉韧带重建后腱骨界面的骨化作用，形成直接止点结构，从而促进腱骨界面的愈合。

关键词: 人脂肪源干细胞, 前叉韧带重建, 腱骨愈合

Abstract:

Objective　To investigate whether human adipose-derived stem cells (hACSs) can promote tendon-bone healing.　Methods　Unilateral anterior cruciate ligament reconstruction using autologous semitendinosus tendons were conducted on 30 New Zealand rabbits(weighing 2~2.5kg), which were randomly divided into 2 groups. Rabbits in the experimental group were injected with hASCs in the tibial and femoral bone tunnels while rabbits in the control group were injected with equal normal saline. All experimental animals were sacrificed at 2 weeks, 4 weeks and 8 weeks after surgery. The specimens were harvested for gross observation and histological analysis.　Results　At 2 weeks after operation in the control group, a lot of inflammatory cells infiltrated the tendon-bone interface while the inflammatory cells decreased at 4 weeks. At 8 weeks postoperatively, sharpey’s fibers were found in the interface forming indirect insertion. In the experimental group, the tendon-bone interface was filled with fibro-cartilage cells and a few osteoblasts at 4 weeks after surgery. New bones can be found in the tendon-bone interface at 8 weeks postoperatively in the experimental group and formed the direct insertion. Conclusion hASCs can promote tendon-bone healing by forming a direct insertion in a rabbit anterior cruciate ligament reconstruction model.

Key words: Human adipose-derived stem cells, Anterior cruciate ligament reconstruction, Tendon-bone healing

陈平,曲戎梅,韩忠宇,杨宇超,尹玉琨,田京,张力. 人脂肪源干细胞促进前交叉韧带重建后腱骨界面愈合的实验研究[J]. 中国临床解剖学杂志, 2016, 34(6): 643-646.

CHEN Ping, QU Rong-mei, HAN Zhong-yu, YANG Yu-chao, YIN Yu-kun, TIAN Jing, ZHANG Li. The study of human adipose-derived stem cells in promoting the tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(6): 643-646.

参考文献

[1] Lohmander LS, Englund PM, Dahl LL, et al. The long-term consequence of anterior cruciate ligament and meniscus injuries: osteoarthritis[J]. Am J Sports Med,2007,35(10):1756-1769.
[2] Spindler KP, Wright RW. Clinical practice. Anterior cruciate ligament tear[J]. N Engl J Med, 2008, 359(20):2135-2142.
[3] Griffin LY, Agel J, Albohm MJ, et al. Noncontact anterior cruciate ligament injuries: risk factors and prevention strategies[J]. J Am Acad Orthop Surg, 2000, 8(3):141-150.
[4] Anderson K, Seneviratne AM, Izawa K, et al. Augmentation of tendon healing in an intraarticular bone tunnel with use of a bone growth factor[J]. Am J Sports Med, 2001, 29(6):689-698.
[5] Sasaki K, Kuroda R, Ishida K, et al. Enhancement of tendon-bone osteointegration of anterior cruciate ligament graft using granulocyte colony-stimulating factor[J]. Am J Sports Med, 2008, 36(8):1519-1527.
[6] Kim JG, Kim HJ, Kim SE, et al. Enhancement of tendon-bone healing with the use of bone morphogenetic protein-2 inserted into the suture anchor hole in a rabbit patellar tendon model[J]. Cytotherapy, 2014, 16(6):857-867.　

[7] Carragee EJ, Hurwitz EL, Weiner BK. A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned[J]. Spine J, 2011, 11(6):471-491.
[8] Lim JK, Hui J, Li L, et al. Enhancement of tendon graft osteointegration using mesenchymal stem cells in a rabbit model of anterior cruciate ligament reconstruction[J]. Arthroscopy, 2004, 20(9):899-910.
[9] Kanaya A, Deie M, Adachi N, et al. Intra-articular injection of mesenchymal stromal cells in partially torn anterior cruciate ligaments in a rat model[J]. Arthroscopy, 2007, 23(6):610-617.
[10] Toma JG, Akhavan M, Fernandes KJ, et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin[J]. Nat Cell Biol, 2001, 3(9):778-784.
[11] Kern S, Eichler H, Stoeve J, et al. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue[J]. Stem Cells, 2006, 24(5):1294-1301.
[12] Rodeo SA, Suzuki K, Deng XH, et al. Use of recombinant human bone morphogenetic protein-2 to enhance tendon healing in a bone tunnel[J]. Am J Sports Med,1999, 27(4):476-488.
[13] Takigami J, Hashimoto Y, Yamasaki S, et al. Direct bone-to-bone integration between recombinant human bone morphogenetic protein-2-injected tendon graft and tunnel wall in an anterior cruciate ligament reconstruction model[J]. Int Orthop, 2015, 39(7):1441-1447.
[14] Zuk P A, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2):211-228.
[15] Hicok KC, Du Laney TV, Zhou YS, et al. Human adipose-derived adult stem cells produce osteoid in vivo[J]. Tissue Eng, 2004,10(3-4):371-380.
[16] Yoon E, Dhar S, Chun DE, et al. In vivo osteogenic potential of human adipose-derived stem cells/poly lactide-co-glycolic acid constructs for bone regeneration in a rat critical-sized calvarial defect model[J]. Tissue Eng, 2007, 13(3):619-627.
[17] Sheykhhasan M, Qomi RT, Ghiasi M. Fibrin scaffolds designing in order to human adipose-derived mesenchymal stem cells differentiation to chondrocytes in the presence of TGF-beta3[J]. Int J Stem Cells, 2015, 8(2):219-227.
[18] An C, Cheng Y, Yuan Q, et al. IGF-1 and BMP-2 induces differentiation of adipose-derived mesenchymal stem cells into chondrocytes-like cells[J]. Ann Biomed Eng, 2010, 38(4):1647-1654.
[19] Jeon O, Rhie JW, Kwon IK, et al. In vivo bone formation following transplantation of human adipose-derived stromal cells that are not differentiated osteogenically[J]. Tissue Eng Part A, 2008, 14(8):1285-1294.
[20] Karaoglu S, Celik C, Korkusuz P. The effects of bone marrow or periosteum on tendon-to-bone tunnel healing in a rabbit model[J]. Knee Surg Sports Traumatol Arthrosc, 2009,17(2):170-178.

人脂肪源干细胞促进前交叉韧带重建后腱骨界面愈合的实验研究

The study of human adipose-derived stem cells in promoting the tendon-bone healing in a rabbit anterior cruciate ligament reconstruction model

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