施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响

doi:10.13418/j.issn.1001-165x.2017.01.010

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (1): 48-51.doi: 10.13418/j.issn.1001-165x.2017.01.010

施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响

付秀美¹，　王荣良²，　杨振江¹，　付文亮¹，　王小杰³

1.承德医学院解剖教研室；　2.承德市妇幼保健院；　3.承德医学院基础所，河北承德 067000

收稿日期:2016-11-07 出版日期:2017-01-25 发布日期:2017-02-22
作者简介:付秀美（1980-），女，讲师，博士, 主要从事周围神经再生修复及人体解剖学研究，Tel：0314-2291137，E-mail:fuxiumei 2012@163.com
基金资助:
河北省教育厅资助项目（QN2014138）; 河北省重点发展学科“人体解剖与组织胚胎学”建设资助项目

Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury

FU Xiu-mei¹, WANG Rong-liang², YANG Zhen-jiang¹, FU Wen-liang¹, WANG Xiao-jie³

1. Department of Anatomy, Chengde Medical College; 2. Chengde Maternal and Child Health Hospital; 3. Institute of Basic Medicine, Chengde Medical College, Chengde Hebei 067000, China

Received:2016-11-07 Online:2017-01-25 Published:2017-02-22

摘要/Abstract

摘要：

目的　观察施万样细胞对坐骨神经损伤（sciatic nerve injury，SNI）大鼠脊神经节NGF 和BDNF 表达的影响，初步探讨施万样细胞对脊神经节的保护作用。方法　先将脂肪源性干细胞（adipose-derived stem cells，ADSCs）诱导分化为施万样细胞并对后者进行鉴定，后将二者分别植入脱细胞神经移植物（ANA）中，构建组织工程神经。大鼠随机分为正常对照组、ADSC 组和施万样细胞组。后两组建立SNI 模型并用相应的组织工程神经桥接损伤的神经。术后4 周采用Western Blot 和Real-time PCR 检测各组大鼠脊神经节神经生长因子（nerve growth factor，NGF）和脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）蛋白和mRNA 的表达。结果　ADSCs 能够诱导分化为施万样细胞并表达施万细胞标记物S100β 和GFAP 蛋白。施万样细胞组大鼠脊神经节内NGF 和BDNF 蛋白及mRNA 表达量均高于ADSC 组（P＜0.05）。结论　施万样细胞可上调脊神经节NGF 和BDNF 的表达，对SNI 所致的脊神经节内神经元损伤有保护作用。

关键词: 施万样细胞, 脊神经节, NGF, BDNF

Abstract:

Objective　To investigate the effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury (SNI) and explore the protective effects of Schwann-like cells on spinal ganglion.　Methods　Firstly，the adipose derived stem cells (ADSCs) were differentiated into Schwann-like cells and the phenotype of the differentiated cells were identified, then the ADSCs and Schwann-like cells were implanted into the acellular nerve grafts (ANA), so the tissue engineering nerves were constructed. The rats were randomly divided into 3 groups: normal control group, ADSC group and Schwann-like cells group. The SNI model was established in rats of the latter two groups, and then the corresponding tissue engineering nerves were used to bridge the injured sciatic nerves. 4 Weeks after operation, Western Blot and Real-time PCR were used to detect the expression of NGF and BDNF protein and mRNA in spinal ganglia.　Results　ADSCs can be induced to differentiate into Schwann-like cells and expression of S100β and GFAP protein (markers of Schwann cells). In contrast with ADSC group, the expressions of NGF and BDNF protein and mRNA in Schwann-like cells group rats increased (P＜0.05). Conclusion　 Schwann-like cells can enhance the expression of NGF and BDNF in the spinal ganglia, so they can have protective effects on the spinal ganglia in rats with SNI.

Key words: Schwann-like cells, Spinal ganglia, Nerve growth factor, Brain-derived neurotrophic factor

付秀美,王荣良,杨振江,付文亮,王小杰. 施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响[J]. 中国临床解剖学杂志, 2017, 35(1): 48-51.

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施万样细胞对大鼠脊神经节NGF 和BDNF 表达的影响

Effects of Schwann-like cells on the expression of NGF and BDNF in spinal ganglia of rats with sciatic nerve injury

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