阿司匹林对血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力的影响

doi:10.13418/j.issn.1001-165x.2017.02.013

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (2): 183-187.doi: 10.13418/j.issn.1001-165x.2017.02.013

阿司匹林对血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力的影响

钮红岺，黄晓萍，　刘晓珑，　李香芝，　肖刚，　刘立新

南方医科大学第三附属医院普外科，广州 510630

收稿日期:2016-12-07 出版日期:2017-03-25 发布日期:2017-04-27
通讯作者: 刘立新，教授，硕士生导师，Tel：020-62784433，E-mail：liulixin@tom.com
作者简介:共同第一作者：钮红岺（1989-），男，河南安阳人，在读硕士，主要从事乳腺癌转移、肝脏慢性炎症对肝癌进展的研究, E-mail：1264433707@qq.com；黄晓萍（1989-），女，广西南宁人，E-mail：huangxp0704@163.com
基金资助:
广州市天河区科技计划项目（201504KW038）

Effect of aspirin on epithelial-mesenchymal transition, migration and invasion in MCF-7 cell induced by platelets

NIU Hong-ling， HUANG xiao-ping， LIU Xiao-long， LI Xiang-zhi， XIAO Gang， LIU Li-xin

Department of General Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China

Received:2016-12-07 Online:2017-03-25 Published:2017-04-27

摘要/Abstract

摘要：

目的　探讨阿司匹林对血小板诱导人乳腺癌MCF-7细胞（MCF-7 human breast cancer cells）上皮间质转化和迁移侵袭能力的影响。方法　以未经干预的MCF-7细胞为对照组，分别用不同浓度（0.5、2.0 mmol/L）的阿司匹林、花生四烯酸激活的血小板及不同浓度阿司匹林预处理花生四烯酸激活的血小板分别干预MCF-7细胞，采用细胞划痕及Transwell实验检测肿瘤细胞迁移侵袭能力的变化。Western Blot检测肿瘤细胞上皮间质转化标记蛋白E-cadherin及Vimentin的表达。结果　活化的血小板处理后与对照组相比MCF-7细胞迁移侵袭能力显著增强（P<0.05），Western Blot显示E-cadherin表达显著减少（P<0.01），Vimentin的表达显著增加（P<0.01）。阿司匹林预处理血小板活化后干预MCF-7细胞，其迁移侵袭能力较活化血小板组显著降低（P<0.05），E-cadherin表达显著增高（P<0.01），Vimentin的表达显著减少（P<0.05）。阿司匹林单独处理后的MCF-7细胞迁移及侵袭能力较对照组无显著变化(P>0.05)，E-cadherin及Vimentin的表达亦无显著改变(P>0.05)。结论　体外实验结果表明，阿司匹林可通过抗血小板活化，抑制血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力。

关键词: 人乳腺癌MCF-7细胞, 阿司匹林, 血小板, 迁移, 侵袭

Abstract:

Objective　To identify the effect of aspirin on epithelial-mesenchymal transition, migration and invasion in MCF-7 cells induced by activated platelets.　Methods　MCF-7 cells were treated with different concentrations of aspirin, platelets activated with arachidonic acid, and different concentrations of aspirin combined platelets activated with arachidonic acid, respectively. Scratch test and transwell test were used to determine the capacities of migration and invasion. Meanwhile, the expression of E-cadherin and Vimentin which linked to epithelial-mesenchymal transition (EMT) was measured by Western Blot.　Results After the intervention of activated platelets, the migration and invasion capabilities of MCF-7 cells were enhanced (P<0.05). The expression of E-cadherin was decreased and Vimentin was increased obviously. After the treatment of low and high concentration aspirin combined with platelets, the migration and invasion capabilities were decreased compared with the group which was treated only with activated platelets(P<0.05). The expression of E-cadherin was elevated and Vimentin was obviouslt reduced. When MCF-7 cells were treated with low concentration (0.5 mmol/l) and high concentration (2.0 mmol/l) of aspirin only,there was no significant difference in the migration and invasion capabilities(P>0.05) compared with the control group without intervention. Meanwhile, Western Blot showed no obvious changes in the expression of E-cadherin and Vimentin (P>0.05).　Conclusion　Results in vitro experiments indicated that aspirin could inhibit the abilities of epithelial-mesenchymal transition (EMT), migration and invasion in MCF-7 cells through suppressing the activation of platelets.

Key words: MCF-7 human breastcancer cells, Aspirin, Platelets activation, Migration, Invasion

钮红岺,黄晓萍,刘晓珑,李香芝,肖刚,刘立新. 阿司匹林对血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力的影响[J]. 中国临床解剖学杂志, 2017, 35(2): 183-187.

NIU Hong-ling， HUANG xiao-ping， LIU Xiao-long， LI Xiang-zhi， XIAO Gang， LIU Li-xin. Effect of aspirin on epithelial-mesenchymal transition, migration and invasion in MCF-7 cell induced by platelets[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(2): 183-187.

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阿司匹林对血小板诱导的乳腺癌MCF-7细胞上皮间质转化和迁移侵袭能力的影响

Effect of aspirin on epithelial-mesenchymal transition, migration and invasion in MCF-7 cell induced by platelets

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