6-羟多巴损伤黑质导致室旁核离子钙接头蛋白表达变化

doi:10.13418/j.issn.1001-165x.2017.02.014

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (2): 188-192.doi: 10.13418/j.issn.1001-165x.2017.02.014

6-羟多巴损伤黑质导致室旁核离子钙接头蛋白表达变化

张毅敏^1,2，　连辉^1,3，　付升旗³，　任同明²，　蔡君艳⁴，　王志勇^1,3

1.河南省医用组织再生重点实验室;　2.新乡医学院人体解剖学实验室;　3.新乡医学院人体解剖学
教研室，河南新乡 453003; 4.东南大学附属中大医院, 江苏南京 210009

收稿日期:2016-11-23 出版日期:2017-03-25 发布日期:2017-04-27
通讯作者: 王志勇，副教授，Tel：0373-3831655，E-mail：wangli heng@vip.163.com
作者简介:张毅敏（1987-），女，河南人，硕士，助理实验师，主要从事神经损伤研究，Tel：0373-3831655，E-mail：zhangyimin2012123@126.com
基金资助:
国家自然科学基金（31200897）;新乡医学院博士科研基金（XYBSKYZZ201501）; 河南省医用组织再生重点实验室开放课题（KFKT16003）

Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine

ZHANG Yi-min ^1,3, LIAN Hui ^2,3, FU Sheng-qi¹, REN Tong-ming², CAI Jun-yan⁴, WANG Zhi-yong^1,3

1.Henan Key Laboratory for Medical Tissue Regeneration，2.Lab of Anatomy, 3.Department of Anatomy, Basic medical, Xinxiang Medical University, Xinxiang 453003, China; 4.Zhongda Hospital, Southeast University, Nanjing 210009, China

Received:2016-11-23 Online:2017-03-25 Published:2017-04-27

摘要/Abstract

摘要：

目的　观察6-羟多巴（6-hydroxydopamine，6-OHDA）损伤大鼠黑质损伤后下丘脑室旁核（paraventricular nucleus of hypothalamus，PVN）中离子钙接头蛋白1（ionized calcium binding adapter molecule-1，Iba1）的含量变化。方法　30只SD大鼠根据随机数字表分为6-OHDA大鼠组和对照组各15只。6-OHDA大鼠组双侧黑质内注射6-羟多巴，对照组双侧黑质（substantia nigra，SN）内注射等体积的生理盐水。6周后断头取脑，Nissl染色、免疫组化、免疫印迹方法鉴定成模，免疫组化、免疫印迹和RT-PCR检测室旁核Iba1的表达。结果　与对照组比较，6-OHDA大鼠组SN内酪氨酸羟化酶（tyrosine hydroxylase, TH）阳性神经细胞数量从（58±5）个减少到（10±2）个（P<0.05），蛋白表达水平从(0.71±0.12)降低至(0.28±0.12)（P<0.05），室旁核中Iba1阳性细胞数量从（7±2）个增加到（14±3）个（P<0.05），蛋白表达水平从(0.1±0.08)增加到(0.19±0.06)（P<0.05）。结论　6-OHDA大鼠PVN中Iba1的表达变化有可能与帕金森病患者的胃肠功能障碍存在一定的联系。

关键词: 帕金森病, 室旁核, 离子钙接头蛋白1

Abstract:

Objective　To observe the expression of ionized calcium binding adapter molecule-1 (Iba1) in rat paraventricular nucleus of hypothalamus (PVN) induced by substantia nigra lesion by 6-hydroxydopamine (6-OHDA).　Methods　According to random number table, a total of 30 male SD rats were randomly divided into a 6-OHDA group and a normal control group. 6-OHDA was injected into the bilateral substantia nigra (SN) of the rats in the 6-OHDA group, and the same volume of saline was injected into the same position of the rats in the normal control group. After sixweeks, the rats were sacrificed and the brains were obtained. Nissl staining was used to detect the Nissl bodies in the SN, and immunofluorescent assay and Western blot analysis were used to detect TH expression in the SN and Iba1 expression in the PVN, and RT-PCR were used to detect Iba1 in the PVN.　Results　Compared with the normal control group, the number of TH immunoreactive neurons in the SN of the 6-OHDA group decreased from (58±5) to (10±2) (P<0.05), and the protein level of TH decreased from (0.71±0.1) to (0.28±0.12) (P<0.05). The number of Iba1 immunoreactive cells in the PVN of the 6-OHDA group increased from (7±2) to (14±3) (P<0.05), and the level of Iba1 protein increased from (0.1±0.08) to (0.19±0.06) (P<0.05).　Conclusion　The changes of Iba1 expression in PVN may be associated with gastric emptying dysfunction in patients with Parkinson’s disease.

Key words: Parkinson's disease, Paraventricular nucleus of hypothalamus, Ionized calcium binding adapter molecule-1

张毅敏,连辉,付升旗,任同明,蔡君艳,王志勇. 6-羟多巴损伤黑质导致室旁核离子钙接头蛋白表达变化[J]. 中国临床解剖学杂志, 2017, 35(2): 188-192.

ZHANG Yi-min, LIAN Hui, FU Sheng-qi, REN Tong-ming, CAI Jun-yan, WANG Zhi-yong. Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(2): 188-192.

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6-羟多巴损伤黑质导致室旁核离子钙接头蛋白表达变化

Alteration of ionized calcium binding adapter molecule-1 in paraventricular nucleus of hypothalamus induced by substantia nigra lesion by 6-hydroxydopamine

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