维生素C对巨噬细胞增殖、迁移及吞噬功能的影响

doi:10.13418/j.issn.1001-165x.2017.03.007

中国临床解剖学杂志 ›› 2017, Vol. 35 ›› Issue (3): 266-270.doi: 10.13418/j.issn.1001-165x.2017.03.007

维生素C对巨噬细胞增殖、迁移及吞噬功能的影响

范志浩¹，　李媛媛¹，　李莉霞¹，　王祥海¹，　潘梦婕¹，　谭丹丹¹文锦坤¹，　钱长晖¹，　李振林¹，　郭家松^1,2,3

1. 南方医科大学基础医学院组织学与胚胎学教研室，广州 510515; 2. 广东省组织构建与检测重点实验室，广州 510515；
3. 广东省骨科研究院骨生物学研究所，广州 510515

收稿日期:2016-12-05 出版日期:2017-05-25 发布日期:2017-06-23
通讯作者: 郭家松，教授，博士生导师，E-mail:jiasongguo@aliyun.com
作者简介:范志浩（1991-），男，山东人，在读硕士，Tel:15626041682， E-mail: fanzhihaokeyan@126.com；李媛媛(1990-)，女，福建人，硕士，Tel: 18620576070， E-mail：yyuan1229@163.com
基金资助:
国家自然科学基金 (81371354, 81571182); 科技部 973 项目(2014CB542202)；广东省科技计划项目 (2015A020212024); 广东省自然科学基金 (S2013010014697)

The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity

FAN Zhi-hao¹, LI Yuan-yuan¹, LI Li-xia¹,WANG Xiang-hai¹, PAN Meng-jie¹, TAN Dan-dan¹, WEN Jin-kun¹, QIAN Chang-hui¹, LI Zhen-lin¹，GUO Jia-song ^1,2,3

1.Department of Histology and Embryology, Southern Medical University, Guangzhou 510515 China; 2.Key Laboratory of Tissue Construction and Detection of Guangdong Province, Guangzhou 510515,China; 3. Institute of Bone Biology, Academy of Orthopedics, Guangdong Province, Guangzhou 510515, China

Received:2016-12-05 Online:2017-05-25 Published:2017-06-23

摘要/Abstract

摘要：

目的　探讨维生素C（Vitamin C, VC）对巨噬细胞增殖、迁移和吞噬能力的影响。方法　体外无菌分离和培养大鼠腹腔巨噬细胞，培养液中加入VC处理24 h，然后利用BrdU和WST技术检测巨噬细胞的增殖能力，荧光微球法检测吞噬功能，划痕实验和Transwell小室检测迁移能力。结果　VC组中巨噬细胞BrdU阳性率和WST吸光度均显著高于对照组(P<0.05)，提示VC能够促进巨噬细胞的增殖；在培养液中加入荧光微球后巨噬细胞能吞噬微球，且VC组巨噬细胞吞噬的微球数量显著多于对照组 (P<0.05)；另外巨噬细胞穿过Transwell小室及划痕后迁移的速度在VC处理后也明显加快(P<0.05)。结论　VC能显著提高巨噬细胞的增殖、迁移与吞噬能力。

关键词: 维生素C, 　巨噬细胞, 　增殖, 　迁移, 　吞噬

Abstract:

Objective　To investigate the effects of Vitamin C (VC) on macrophage proliferation, migration and phagocytic activity.　Methods　Macrophages isolated from SD rats were cultured in a basic medium supplement with or without VC for 24 hours. Then the proliferation of macrophages was assessed by BrdU and WST assays, while the phagocytic activity was detected by the phagocytosis of lumispheres which were administrated into the culture medium for 3 hours, and the migration was measured by scratching test and Transwell assay.　Results　The percentage of BrdU positive cells was significantly increased in the VC treated group while the intensity of WST test was higher in VC group than that of control group (P<0.05). The number of lumispheres in each macrophage of VC group was much more than that of control group(P<0.05). Moreover, VC could accelerate the migrating rate of macrophages when the cells were tested by scratching test and Transwell assay (P<0.05).　Conclusion　VC could enhance the proliferation, migration and phagocytosis of macrophages.

Key words: Vitamin C, 　Macrophage, 　Proliferation, 　Migration, 　Phagocytic activity

范志浩,李媛媛,李莉霞,王祥海,潘梦婕,谭丹丹,文锦坤,钱长晖,李振林,郭家松. 维生素C对巨噬细胞增殖、迁移及吞噬功能的影响[J]. 中国临床解剖学杂志, 2017, 35(3): 266-270.

FAN Zhi-hao, LI Yuan-yuan, LI Li-xia,WANG Xiang-hai, PAN Meng-jie, TAN Dan-dan, WEN Jin-kun, QIAN Chang-hui, LI Zhen-lin，GUO Jia-song. The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(3): 266-270.

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维生素C对巨噬细胞增殖、迁移及吞噬功能的影响

The effect of Vitamin C on macrophage proliferation, migration and phagocytic activity

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