关键词: 脂多糖, 　迷走神经背核, 　多巴胺受体1, 　多巴胺受体2

Abstract:

Objective    To observe the expression of tyrosine hydroxylase (TH)、dopamine receptor 1 (DR1) and dopamine receptor 2 (DR2) in rat dorsal motor nucleus of vagus (DMV) induced by substantia nigra (SN) lesion by lipopolysaccharide (LPS).　Methods　According to the random number table, a total of 30 female SD rats were randomly divided into a LPS group and a normal control group. LPS was injected into the bilateral SN of the rats in the LPS group, and the same volume of saline was injected into the same position of the rats in the normal control group. After six weeks, rotating rod experiment, gastric emptying, immunofluorescent assay and western blotting analysis were used to detect TH expression in the SN and TH, DR1 and DR2 expression in the DMV.　Results　Six weeks later，the LPS group rats were identified successfully, and the gastric emptying ability was reduced, the number of TH immunoreactive neurons in the DMV of the LPS group increased from (7±3) to (12±2) (P<0.05), the number of DR1 immunoreactive neurons decreased from (28±5) to (18±6) (P<0.05) and the number of DR2 immunoreactive neurons increased from (20±3) to (33±4) (P<0.05). In the dorsal medulla, the level of TH protein increased from (0.16±0.02) to (0.43±0.04) (P<0.05), the level of DR1 protein decreased from (0.78±0.05) to (0.36±0.03) (P<0.05) and the level of DR2 protein increased from (0.43±0.03) to (0.58±0.07) (P<0.05).　Conclusion　The changes of TH, DR1 and DR2 expression in DMV of LPS group rats may be associated with gastric emptying dysfunction in patients with Parkinson’s disease.

Key words:  LPS；　Dorsal motor nucleus of vagus；　Dopamine receptor 1;   , Dopamine receptor 2