Abstract:

Objective　To study the protection of total Panax Notoginseng Saponins on neurons in the areas surrounding the lesion of intracerebral hemorrhage（ICH）in rats.　Methods　ICH model was established by injecting collagenase Ⅶ into the brain of rats. The ultrastructure of neurons that surround intracerebral hemorrhage in rats was observed by transmission electron microscope. Immunohistochemical staining technique was used to demonstrate expressions of Caspase-3 protein and TUNEL protein. Berderson score was adopted to evaluate behavioral change.　Results　The result of immunohistochemistry showed that the expression of Caspase-3 protein and TUNEL protein in the treatment group was significantly reduced (P<0.01). The ultrastructure of neuron in the normal group was common, which, however, was seriously destroyed in model group. The ultrastructure of neuron in treatment group improved obviously compared with that of the model group.　Conclusion　Total Panax Notoginseng Saponins could protect neurons located near the hemorrhagic area of the brain.

Key words:  Total Panax Notoginseng Saponins, Intracerebral hemorrhage, Neurons, Apoptosis, Rat