LPS直接及间接作用致小鼠急性肺损伤模型的建立

doi:10.13418/j.issn.1001-165x.2015.04.014

Abstract

Abstract:

Objective　Acute lung injury(ALI) that results from acute inflammation of respiratory system is a popular acute and serious pulmonary symptom in clinic. The successful establishment of the mouse model for ALI is essentially important.　Methods　In this study, we compared the differences between injection LPS from intratrecher (i.t.) and intraperitoneal (i.p.). By histological and pathological evaluation, cytologic reaction of inflammation cells. Change of protein secreted by inflammation cells were adopted to rate the effectiveness of model establishment induced by i.t. and i.p. injection.　Results　We got more obvious inflammation reaction and more efficient ALI by i.t. injection. On cytologics item, we got more exudation of neutrophil cells in BALF (bronchial alveoli lavage fluid) by i.t. injection. On cytokines item, secreted proteins were obviously increased in bronchoalveoli lavage fluid by i.t. injection. Conclusions Injection LPS by i.t. is a better method for establishment of ALI model, which leads to acute lung injury directly and immediately.

Key words: LPS, Acute Lung Injury(ALI), Intratrecher(i.t.), Intraperitoneal (i.p.)

References

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Building the acute lung injury mouse model by LPS direct and indirect challenge

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