长期高脂饮食对大鼠下丘脑腹内侧核、弓状核PGC-1α表达的影响

doi:10.13418/j.issn.1001-165x.2015.05.016

Abstract

Abstract:

Objective　To investigate the role of high fat diet in the distribution of PGC-1α in SD rat hypothalamus. Methods 12 SD rats were randomly divided two groups, a normal diet group and a high fat diet group. HE staining was used to detect the fatty degeneration of the liver tissue; Immunofluorescence staining was used to observe the change of PGC-1α in the hypothalamusventromedial nucleus, and arcuate nucleus after 8 weeks.　Results　8 weeks later, the live tissue of normal diet rats showed that hepatic cord was well organized; the boundary among hepatic cord, liver blood sinus and liver cells was clear; the nucleus were clear and cytoplasmhomogeneous; there was no liver cell lipid and necrosis. But the high fat diet rat live tissues expressed the characteristic of serious fatty degeneration, such as hepatic corddisorganized, liver blood sinus cavity became smaller, the boundary was not clear, live cells swelling with fat vacuoles in the cytoplasm which was not uniform in size. The PGC-1α cell number in the normal diet rat hypothalamusventromedial nucleus determination area was (199.96±42.95), but it was (256.08±39.25) in the high fat diet rat, and there was statistical significance(P<0.05). In the arcuate nucleus the cell number of PGC-1α was (173.25±47.19) in the normal diet rat, but high fat diet made the cell number increased to 200.48±51.44, and there was statistical significance between them( P<0.05).　Conclusion　High fat diet made the cell number of PGC-1α increased in ventromedial nucleus andarcuate nucleus, PGC-1α may play a role in regulating the energy metabolism balance.

Key words: PGC-1α, Hypothalamusventromedial nucleus, Arcuate nucleus, High fat diet

References

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The distribution and significance of PGC-1α neurons in the centre of energy homeostasis in hypothalamus

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