骨髓间充质干细胞对髋部骨折诱导的老年大鼠急性肺损伤的保护机制

doi:10.13418/j.issn.1001-165x.2015.06.012

Abstract

Abstract:

Objective　To investigate the protective effect of bone marrow-derived mesenchymal stem cells on acute lung injury (ALI) after unilateral hip fracture in elderly rat.　Methods　15 month old WISTAR rat were randomly divided into three groups: a control group (n=15)、a model group(hip fracture+NS, n=15) and a treated group (hip fracture+BMSCs, n=15). 1 ml BMSCs（2.5×106/ml）were injected through the caudal vein in the treated group, and the same volume of NS was injected into the model group Histologic changes were examined at 24 hours after fracture. And the concentration of MPO and related inflammatory factors in the impaired lung tissue and blood were examined through enzyme-linked immunosorbent assay (ELISA).　Results　Compared with the control group,the histopathological damage of lung, the concentration of MPO and related inflammatory factors were obviously enhanced（P<0.01）. Compared with the model group,application of BMSCs significantly ameliorated the inflammatory lesions of the lung, increased the concentration of IL-10, and further down-regulated the concentration of MPO and other inflammatory factors（P<0.01）.　Conclusions　ALI can be induced by hip fracture in aged rats. BMSCs shows a certain protective effect on ALI with down-regulation of inflammatory response in systemic and pulmonary as a possible mechanism.

Key words: Aged rats, Hip fracture, Bone marrow-derived mesenchymal stem cells, Acute lung injury

References

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The protective effect of bone marrow-derived mesenchymal stem cells on acute lung injury after hip fracture in elderly rat

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