Abstract:

Objective　To compare the difference of the time-validity of repairing and the fluorescence stability between BMSCs infected by a lentiviral vector carrying GFP and the BMSCs in GFP rats in treatment of hepatic ischemia reperfusion injury. 　Method　The two kinds of BMSCs were cultured and the method of MTT was used to detect the differences of the two cell growth curves. Healthy SD rats were randomly divided into a sham operation group、a lentiviral transfection GFP group(LV-GFP group)、a GFP transgene group(GFP-BMSCs group) and a model group. After operation, the rats of the LV-GFP group and the GFP-BMSCs group were immediately injected with the corresponding cell suspension of 200 μl (the number of 1×106) by portal vein, and the model group was injected with the same volume of PBS. After 1, 2, 3 and 4 weeks，the levels of AST、ALT and ALB were tested by the blood sampling in different groups. From 1 to 5 days after operation, the hepatic tissue from the experience groups were transected to observe the migrating of the GFP positive cells in the liver; At  week 4, the hepatic tissue was transected from the experience groups to detect the fluorescence stability of the BMSCs and its expression of the keratin 18 (CK18) in the liver.　Results　The proliferation of the BMSCs in GFP rats was significantly stronger than that of BMSCs infected by a lentiviral vector carrying GFP in logarithmic phase. At weeks 1 and 2, the levels of the aspartate amino transferase (AST) and alanine amino transferase (ALT) in GFP-BMSCs group were lower than those in LV-GFP group (P<0.05), and the level of serum albumin (ALB) in GFP-BMSCs group was higher than that in LV-GFP group at  weeks 2 and 3 (P<0.05). BMSCs labeled GFP in hepatic lobules were successively found in the GFP-BMSCs group and the LV-GFP group on the third and 5th day, respectively. The hepatic cells from BMSCs cells in the liver were found in the GFP-BMSCs group and the LV-GFP group at week 4 after operation, but the fluorescence intensity in GFP-BMSCs group was better than that in LV-GFP group.　Conclusion　The BMSCs in GFP rats show the better time-validity of repairing and fluorescence stability than the BMSCs infected by a lentiviral vector carrying GFP in treatment of hepatic ischemia reperfusion injury.

Key words: Bone marrow mesenchymal stem cells, Green fluorescence protein, Hepatic ischemia reperfusion injury, Lentiviral