糖尿病神经病理性疼痛大鼠脑异常活动的功能磁共振研究

doi:10.13418/j.issn.1001-165x.2016.04.011

Abstract

Abstract:

Objective　To construct the diabetic neuropathic pain rat model. To detect the functional activity changes of brain and explore the brain regions with abnormal function activities using functional magnetic resonance imaging. Methods Forty-eight male SD rats were chosen and randomly divided into two groups: a diabetes group (n=36) and a control group (n=12). Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg) with observation of the changes of fasting blood glucose level and body weight. Behavioral testing for paw 50% withdrawal threshold was performed at the same time. Tactile allodynia was defined as a 50% withdrawal threshold of less than or equal to 2.0 g. According to the tactile allodynia, the diabetes group was sorted into two groups: the pain group and painless group. Functional activity mapping of brain by manganese enhanced magnetic resonance imaging and voxel-wise t-test were then applied to statistically analyze the brain regions with abnormal function activities in diabetic neuropathic pain rat. Results Diabetic neuropathic pain rat was successfully induced. some brain regions, including the somatosensory cortex, ventromedial prefrontal cortex, anterior cingulate cortex, inferior olive, piriform cortex,and part of the cortexes of the amygdala and insula, were found with significantly enhanced activity in diabetic neuropathic pain rat. Conclusions The results suggest these brain regions with abnormal function activities probably take part in the regulation of diabetic neuropathic pain within central nervous system.

Key words: Diabetes, Pain, Functional magnetic resonance imaging, Abnormal function activity, Brain

WU Bo-lin, FA Zhi-qiang, LAI Chun-ren, GUO Sheng-wen, GUO Yan-wu. Abnormal activity of brain detected by functional magnetic resonance imaging in a rat model of diabetic neuropathic pain[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(4): 407-411.

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Abnormal activity of brain detected by functional magnetic resonance imaging in a rat model of diabetic neuropathic pain

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