丝线法制备豚鼠不完全性小肠梗阻模型及对c-kit表达的影响研究

doi:10.13418/j.issn.1001-165x.2016.04.014

Abstract

Abstract:

Objective　To investigatethe expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models in guinea pig with the silk method.　Methods　43 adult healthy guinea pigs were selected and randomly divided into the normal group A, sham-operated group B, model groups（C, D, E）. Model groups were established for incomplete small intestinal obstruction by the means of silk method for 3 days. After taking out of the silk and closing the abdominal cavity, the abdominal cavity was opened and the intestinal contractility was measured，respectively at 1st（C group）, 3rd（D group）,7th（E group）. Meanwhile, the contractility of intestinal tissue was measured, the degree of intestinal mucosal membrane injury was evaluated and the expression of c-kit was observed.　Results　The comparison of normal and model groups in every data’s measurement were significant on statistics (P<0.05),but the comparison of intestinal mucosal membrane injury between E and A group had no significant difference (P>0.05). The contractility of intestinal tissue of E group was higher than D group (P<0.05), and the degree of intestinal mucosal membrane injury was less serious than D group (P<0.01). Besides, the expression of c-kit in E group wass significantly higher than D group (P<0.01). Conclusion　Incomplete intestinal obstruction with down-regulated c-kit could be established by the silk method.

Key words: Interstitial cells of Cajal, Silk method, c-kit, Guinea pig, Incomplete small intestinal obstruction

YANG Zheng, LI Xin-rui, WANG Qiang, LIU Shuang, HUANG Qian, JI Zhen-hua. Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(4): 423-427.

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Observe the expression of c-kit gene in intestinal cells of Cajal by establishing incomplete small intestinal obstruction models of guinea pig with silk method

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